Enterokinase Cleavage Enzyme
BackEnterokinase
Order Online
Order by Contact
| Email: | order@abmGood.com |
| Phone: | 604-247-2416 (Local), 1-866-757-2414 (Toll free) |
| Fax: | 604-247-2414 |
| Payment: |   or Purchase Order Number |
| More info: | Additional Information Regarding Orders |
| Cat. No. | Product Name | Qty. | Promo/% Off | Amount | ||
|---|---|---|---|---|---|---|
| Total: | $0.00 | |||||
| *Notes about Promo Codes: For NON-DISCOUNT promo codes (i.e. free items or shipping) please enter them during check out in the Notes/Promo Code field. To enter a DISCOUNT promo code enter it into the corresponding box in the 'Promo/% Off' column and then click 'Update' for the given product. If the code is valid for that product the discount percentage will display in place of the box and the amount will become the discounted total for that product(s). If the code is not valid it will remain in the box, please double check the expiry and spelling, and if you feel the code should still be accepted please contact us. | ||||||
Detailed Information
Description
The enterokinase included in this kit is the catalytic
subunit of the native holoenzyme, and is highly active and specific for cleaving fusion proteins with the recognition sequence, DDDDK, in the interdomain linker.
Because this product is produced from mammalian expression system, it is highly glycosylated and shows extremely specific cleavage activity compared to other E. coli produced enterokinases. The purified enterokinase behaves as a 47kD band under denaturing and reducing conditions as visualized on SDS-PAGE.
subunit of the native holoenzyme, and is highly active and specific for cleaving fusion proteins with the recognition sequence, DDDDK, in the interdomain linker.
Because this product is produced from mammalian expression system, it is highly glycosylated and shows extremely specific cleavage activity compared to other E. coli produced enterokinases. The purified enterokinase behaves as a 47kD band under denaturing and reducing conditions as visualized on SDS-PAGE.
Applications
Enterokinase is a site-specific protease that recognizes and cleaves after the C-terminal end of lysine residue in the recognition sequence, DDDDK. Unlike other site-specific proteases that cut within the recognition sequences leaving extra amino acids in the cleaved peptide products, the C-terminal peptide fragment produced from the enterokinase cleavage reaction doesn't inherent any residues from the DDDDK recognition sequence*. Therefore, the application can be extremely advantageous for producing a 100% native protein sequence and structure from recombinant fusion protein,
which has the desired product immediately after the enterokinase recognition sequence, DDDDK. [*Note: Enterokinase will not cleave at site when lysine is followed by proline.]
In addition, DDDDK is part of the octapeptide FLAG tag (DYKDDDDK), which has been used as a fusion tag for recognition by antibody and detection of fusion protein expression with Western blot analysis, and for purification of the fusion protein by Anti-FLAG affinity chromatography. This array of applications makes enterokinase an ideal tool in the research involving the study of protein structure and function, and protein production where native protein structures and sequences are desired.
which has the desired product immediately after the enterokinase recognition sequence, DDDDK. [*Note: Enterokinase will not cleave at site when lysine is followed by proline.]In addition, DDDDK is part of the octapeptide FLAG tag (DYKDDDDK), which has been used as a fusion tag for recognition by antibody and detection of fusion protein expression with Western blot analysis, and for purification of the fusion protein by Anti-FLAG affinity chromatography. This array of applications makes enterokinase an ideal tool in the research involving the study of protein structure and function, and protein production where native protein structures and sequences are desired.
Components
Special Features
Data Sheet 
References
FAQs