Ni-IDA Agarose Beads ($55.00) | Protein Purification

Protein Purification

Ni-IDA Agarose Beads

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Product Name	Size	Cat. No.	Price
Ni-IDA Agarose Resins for Protein Purification	5ml	G250	$55.00
Ni-IDA Agarose Resins for Protein Purification	25ml	G251	$195.00
Ni-IDA Agarose Resins for Protein Purification	100ml	G253	$545.00
Ni-IDA Agarose 1ml Column for Protein Purification	8x1ml	G239	$175.00
Ni-IDA Agarose 5ml Column for Protein Purification	5x5ml	G240	$475.00

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Detailed Information

Description

Affinity chromatography is a method of separating biochemical mixtures based on highly specific molecular interactions. The Ni-IDA agarose is developed for the purification of proteins with an affinity tag of six consecutive histidine residues. This histidine-metal ion interaction makes one-step purification possible for proteins from any expression system, under native or denaturing conditions. The His-tag sequence binds to Ni²⁺ cations, which are immobilized onto a solid support using iminodiacetic acid groups (IDA). Impurities are removed and the purified His-tagged proteins can be eluted by imidazole or a reduced pH solution.

Applications

Purification of His-tagged proteins.

Antibody purification.

Assays involving protein-protein and protein-DNA interactions.

Structural and functional investigation of proteins.

Special Features

One-step easy purification protocol from crude lysate to >95% pure protein.

Purification under native or denaturing conditions.

Ready-to-use for any scale of purification.

Protein binding capacity: approx. 20-40 μmol Ni²⁺/ml gel.

Economical value.

Can be used for successive cycles.

Resins and columns formats suitable for batch and gravity purification.