Description

  With years of experiences with cell line cell immortalization, scientists at ABM have been able to successfully immortalize essentially any human and mouse cell in last 10 years using different immortalizing agents or genes. If you have a primary cell culture that needs to be immortalized, our custom services is the quick and reliable service for your project. It saves you time and money over trying different genes yourself. Our custom service package already includes all different genes and you will not pay anything unless your cells are successully immortalized. So it is risk-free and you pay only when your cell line is immortalized.

We not only provide cell immortalization custom service, but also sell cell immortalization products.

Ordering

Contact us for quotation of our cell immortalization services.
Tel: 1-866-757-2414 (Toll Free) , 604-247-2416 (Local)
Email: order@abmgood.com

How do I send my cells and place an order? and What will I be receiving?

For frozen cells, you must ship in ample dry ice. Another method is to send us two T25 flask of live cells at 90% confluency. The flask should be filled with complete medium without any air bubble and at room temperature. The flask can be filled with medium using a 50ml culture tube. Make sure that sterilization procedure is strictly observed. For North American customers, the cells should be sent on Monday, Tuesday or Wednesday. For non-North American customers, the cells should be sent on Friday or Monday to avoid transit delay over the weekend.


If your cells require medium other than DMEM and RPMI, we will need 1 L of medium and any applicable growth factors from you for the project. Also, please provide coated 6-well plates and T25 flasks if the cells require specially treated culture vessels.

Once you have shipped your cells, please forward us the tracking number for custom clearance.

Once we receive the cells, we will performed custom cell immortalization in 6-well plates with all our cell immortalization reagents, including SV40 T antigen, hTERT, Myc, E6/E7.

We will make sure the cells are immortalized and have the ability to grow forever. The final product to be shipped to you with be two to three different clones that have been successfully immortalized.

Can I purchase immortalized human neurons? I don't have primary neurons, but rather you can provide both the primary cell and the immortalization service?

Yes, we will be able to try to immortalize those cells for you. Please let us know your specific requirement and we will send you an official quotation.

How many cells do I need to send in order to immortalize one cell line?

Please send enough cells to fill 3xT25 flasks at 70% density, for each cell line that you wish to immortalize.

What should I do if my cells do not grow using standard media?

If your cells do not grow using DMEM + 10% FBS or RPMI + 10% FBS, then we need at least 1L of your growth media plus additional amounts of associated growth factors.