SafeView™
BackSafeView™
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Data Sheet
FAQs
- Can SafeView be a replacement for ethidium bromide? Can I do gel extraction with it?
- How does SafeView work and how come it is not carcinogenic?
- How do I use SafeView products?
- Does the SafeView differentiate double stranded nucleic acid and single stranded nucleic acid? Does the Safe-(Red, Green and White) work the same way?
- At what temperature do I store the SafeView products?
- Do I need a special filter for photography of DNA gels stained with SafeView?
- How long does the SafeView Classic stain last in a gel?
- Why is SafeView (G-108) stain not working on my samples?
- How long does it take to the stain to dissociate from Nucleic Acids?
- Is it degradable, if so how fast and under what circumstances?
- What is the sensitivity of the dyes?
- Can SafeView products be used post-stain?
- Why is the EtBr signal stronger in the pictures when I compare SafeView with EtBr?
Can SafeView be a replacement for ethidium bromide? Can I do gel extraction with it?
SafeView can be used as a replacement for ethidium bromide as they both work on general agarose. We have clients who buy our SafeView products and use it for cloning purposes, and they have no problem with it.
How does SafeView work and how come it is not carcinogenic?
There are fluorescent compounds in SafeView and these fluorescent compounds have the capability to bind to DNA. There may be some unknown effects of SafeView that have not been documented but that applies to the SYBR set as well; however, SafeView products are definitely not as carcinogenic as ethidium bromide.
How do I use SafeView products?
The Safe-(Red, Green and White) loading dyes work the same as 6x loading dye, loaded with the sample. SafeView Classic is used directly in the gel and the running buffer.
Does the SafeView differentiate double stranded nucleic acid and single stranded nucleic acid? Does the Safe-(Red, Green and White) work the same way?
Yes, SafeView Classic can differentiate double stranded (green fluorescence) and single stranded (red fluorescence). The Safe-(Red, Green and White) cannot do that.
At what temperature do I store the SafeView products?
All the SafeView products should be stored at four degrees Celsius.
Do I need a special filter for photography of DNA gels stained with SafeView?
If you want to take a black and white picture of the gel, there is no need for the filter. If you want the original colours of the bands, then you would need a filter for the photo.
How long does the SafeView Classic stain last in a gel?
We have not tested the duration of the SafeView Classic stain on the gel but we can say it is comparable to ethidium bromide. As for the buffer, it is recommended to add more SafeView Classic stain every 5 gels to maintain a strong signal.
Why is SafeView (G-108) stain not working on my samples?
Make sure you are following the protocol carefully and adding 5ul of SafeView for every 100ml of running buffer and gel. It is critical that both buffer and gel have SafeView dye in them otherwise it will not work. This is different from ethidium bromide.
How long does it take to the stain to dissociate from Nucleic Acids?
It will take one hour of washing with water.
Is it degradable, if so how fast and under what circumstances?
2 hours over 100C
What is the sensitivity of the dyes?
Safe-Green has Excitation Wavelength of 290nm and Emission Wavelength of 490nm, and its sensitivity is range between 0.2-0.6ng
Safe-Red has Excitation Wavelength of 490nm and Emission Wavelength of 630nm, and its sensitivity is range between 0.3-0.8ng
Safe-White has Excitation Wavelength of 320nm and Emission Wavelength of 480nm, and its sensitivity is range between 0.2-0.5ng
SafeView Classic has Excitation Wavelength of 290nm and Emission Wavelength of 605nm and the its sensitivity is range between 0.1-0.3ng
SafeView Plus has Excitation Wavelength of 290nm and Emission Wavelength of 520nm and the its sensitivity is range between 0.05-0.1ng
Safe-Red has Excitation Wavelength of 490nm and Emission Wavelength of 630nm, and its sensitivity is range between 0.3-0.8ng
Safe-White has Excitation Wavelength of 320nm and Emission Wavelength of 480nm, and its sensitivity is range between 0.2-0.5ng
SafeView Classic has Excitation Wavelength of 290nm and Emission Wavelength of 605nm and the its sensitivity is range between 0.1-0.3ng
SafeView Plus has Excitation Wavelength of 290nm and Emission Wavelength of 520nm and the its sensitivity is range between 0.05-0.1ng
Can SafeView products be used post-stain?
No, SafeView products are not designed for post-stain. SafeView Classic must be added to the gel and the running buffer prior to the loading of the samples. Safe-(Red, Green and White) dyes must be added to the sample before loading it to the gel.
Why is the EtBr signal stronger in the pictures when I compare SafeView with EtBr?
A reason for this is that most gel doc systems have been optimized for EtBr so that is why the EtBr signal is so strong in the pictures.
How do I dispose Agarose Gels stained with Safeview and how do I dispose buffers and gloves?
Dispose SafeView™ Classic as you would any other non-carcinogenic fluorescent dye (eg. Acridine orange; Propidium iodide).
All gels and contaminated “non-sharp” lab debris (e.g., gloves, pads, towels, tubes, etc.) that are processed using this dye can be discarded in the trash.
Spent running buffer solutions and destaining solutions that contain the dyes can either be collected and disposed of through the HWMU or collected and run through an approved filter device.
The buffer solutions that have been run through the approved filter should be checked under the appropriate light source for complete removal of the dyes, and if it passes (does not fluoresce), the liquid can be disposed of down the drain with a copious amount of water as long as no other materials are present that would cause the material to be a Hazardous Waste.
The filters that have been used up and are no longer effective must be disposed of through the HWMU.
All gels and contaminated “non-sharp” lab debris (e.g., gloves, pads, towels, tubes, etc.) that are processed using this dye can be discarded in the trash.
Spent running buffer solutions and destaining solutions that contain the dyes can either be collected and disposed of through the HWMU or collected and run through an approved filter device.
The buffer solutions that have been run through the approved filter should be checked under the appropriate light source for complete removal of the dyes, and if it passes (does not fluoresce), the liquid can be disposed of down the drain with a copious amount of water as long as no other materials are present that would cause the material to be a Hazardous Waste.
The filters that have been used up and are no longer effective must be disposed of through the HWMU.
Do SafeView products give problems in the process of cloning?
No, SafeView products are fine for cloning. We use them routinely in-house for our cloning work.