149RM Cells

CAT.NOUNITPRICE
T83301x106 cells / 1.0 ml
$0.00

Specifications


Description

149RM cells were derived from a male human with penile squamous cell carcinoma. The cell line is tuminorgic in mice and has a very high proliferation rate and metastasis potential. The 149RM cells are sensitive to cisplatin however they are resistant to anti-EGFR therapy and unresponsive to androgen. The 149RM cell line may be used for further investigation of penile squamous cell carcinoma and development of relevent therapeutic treatment. 

SKUT8330
SpeciesHuman (H. sapiens)
Donor GenderMale
Donor DiseasePenile squamous cell carcinoma
Growth PropertiesAdherent
Cell MorphologyEpithelial-like
Seeding Density30,000 - 40,000 cells/cm2; Recommended split ratio is 1:3 or 1:8
Applications

For Research Use Only

Unit quantity1x106 cells / 1.0 ml
Cell TypeTumor Cells
Propagation Requirements

Use of PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. PriGrow III (TM003) + 10% FBS + 1% Penicillin/Streptomycin Solution (G255), 37.0°C, 5% CO₂

Disclaimer

1. For for-profit organizations and corporations, please contact [email protected] for pricing of this item.

2. Sale of this item is subjected to the completion of a Material Transfer Agreement (MTA) by the purchasing individual/institution for each order. If you have any questions regarding this, please contact us at [email protected].

3. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 μg, Cat.# C207, $450.00) or cell lysate (100 μg, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final.

4. We recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping).

5. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information.

6. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate.

7. abm warrants that cell lines shall be viable upon initiation of culture for a period of thirty (30) days after shipment and that they shall meet the specifications on the applicable abm Material Product Information sheet, certificate of analysis, and/or catalog description. Such thirty (30) day period is referred to herein as the "Warranty Period."

DepositorSun Yat-sen University Cancer Center (Hui Han)
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      References


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      • Chen, J., Yao, K., Li, Z., Deng, C., Wang, L., Yu, X., Liang, P., Xie, Q., Chen, P., Qin, Z., Ye, Y., Liu, Z., Zhou, F., Zhang, Z., & Han, H. (2016). Establishment and characterization of a penile cancer cell line, penl1, with a deleterious TP53 mutation as a paradigm of HPV-negative penile carcinogenesis. Oncotarget, 7(32), 51687–51698. https://doi.org/10.18632/oncotarget.10098
      • Zhou, Q. H., Deng, C. Z., Li, Z. S., Chen, J. P., Yao, K., Huang, K. B., Liu, T. Y., Liu, Z. W., Qin, Z. K., Zhou, F. J., Huang, W., Han, H., & Liu, R. Y. (2018). Molecular characterization and integrative genomic analysis of a panel of newly established penile cancer cell lines. Cell death & disease, 9(6), 684. https://doi.org/10.1038/s41419-018-0736-1
      • Hitora, T., Yamamoto, T., Akisue, T., Marui, T., Nakatani, T., Kawamoto, T., ... & Kurosaka, M. (2005). Establishment and characterization of a KIT‐positive and stem cell factor‐producing cell line, KTHOS, derived from human osteosarcoma. Pathology international, 55(2), 41-47.
      • Oshimo, Y., Munetomo, A., Magata, F., Suetomi, Y., Sonoda, S., Takeuchi, Y., Tsukamura, H., Ohkura, S., & Matsuda, F. (2021). Estrogen increases KISS1 expression in newly generated immortalized KISS1-expressing cell line derived from goat preoptic area. The Journal of reproduction and development, 67(1), 15–23. https://doi.org/10.1262/jrd.2020-053