293 Cell Lysate
|L032||100 μg / 100 μl|
|Description||Human Embryonic Kidney Adenovirus Transformed Cells|
|Lysate Type||Cell Line Lysate|
|Unit quantity||100 μg / 100 μl|
|I tried the Tag antibody on the corresponding lysate but do not get any signal on a western blot. Why?|
Ensure the correct secondary antibody was used. Also, to get a good signal, try loading higher amounts of the lysate (such as 20ug) to strengthen the signal.
- Lloyd, T. E. et al. "Cytosolic 5′-Nucleotidase 1A As a Target of Circulating Autoantibodies in Autoimmune Diseases" Arthritis Care & Research 68(1): 66-71 (2015). DOI: 10.1002/acr.22600. Application: Cat. No. L032.