293 Cell Lysate
| CAT.NO | UNIT | PRICE |
|---|---|---|
| L032 | 100 μg / 100 μl | $180.00 |
| Description | Human Embryonic Kidney Adenovirus Transformed Cells |
|---|---|
| SKU | L032 |
| Lysate Type | Cell Line Lysate |
| Note | The ready-to-use, whole-cell lysate is available as a positive control for Western blot analysis. Recommended loading volume per lane is 5-10ul depending on the size format of your gel. All lysates should be boiled once prior to use. No additional loading dye is needed prior to loading. |
| Shipping Conditions | Blue Ice |
| Storage Condition | Buffer: SDS, Tris HCl, glycerol, bromophenol blue, DTT. Store at -20°C. Avoid freeze-thaw cycles. |
| Unit quantity | 100 μg / 100 μl |
Supporting Protocol
MSDS
QC
Other
| I tried the Tag antibody on the corresponding lysate but do not get any signal on a western blot. Why? | |
Ensure the correct secondary antibody was used. Also, to get a good signal, try loading higher amounts of the lysate (such as 20ug) to strengthen the signal. | |
1
- Lloyd, T. E. et al. "Cytosolic 5′-Nucleotidase 1A As a Target of Circulating Autoantibodies in Autoimmune Diseases" Arthritis Care & Research 68(1): 66-71 (2015). DOI: 10.1002/acr.22600. Application: Cat. No. L032.