293A Cell Lysate

CAT.NOUNITPRICE
L165100 μg / 100 μl
$180.00

Specifications


DescriptionHuman Embryonic Kidney Adenovirus Transformed Subclone Cells
SKUL165
Lysate TypeCell Line Lysate
NoteThe ready-to-use, whole-cell lysate is available as a positive control for Western blot analysis. Recommended loading volume per lane is 5-10ul depending on the size format of your gel. All lysates should be boiled once prior to use. No additional loading dye is needed prior to loading.
Shipping ConditionsBlue Ice
Storage ConditionBuffer: SDS, Tris HCl, glycerol, bromophenol blue, DTT.
Store at -20°C. Avoid freeze-thaw cycles.
Unit quantity100 μg / 100 μl
Documents


Supporting Protocol

    MSDS

      QC

        Other

          FAQs


          I tried the Tag antibody on the corresponding lysate but do not get any signal on a western blot. Why?
          Ensure the correct secondary antibody was used. Also, to get a good signal, try loading higher amounts of the lysate (such as 20ug) to strengthen the signal.
          References


          1
          • Lloyd, T. E. et al. "Cytosolic 5′-Nucleotidase 1A As a Target of Circulating Autoantibodies in Autoimmune Diseases" Arthritis Care & Research 68(1): 66-71 (2015). DOI: 10.1002/acr.22600. Application: Cat. No. L032.