293T Cell Lysate
|L033||100 μg / 100 μl|
|Description||SV40 Large T Antigen 293 Cells|
|Lysate Type||Cell Line Lysate|
|Note||The ready-to-use, whole-cell lysate is available as a positive control for Western blot analysis. Recommended loading volume per lane is 5-10ul depending on the size format of your gel. All lysates should be boiled once prior to use. No additional loading dye is needed prior to loading.|
|Shipping Conditions||Blue Ice|
|Storage Condition||Buffer: SDS, Tris HCl, glycerol, bromophenol blue, DTT.|
Store at -20°C. Avoid freeze-thaw cycles.
|Unit quantity||100 μg / 100 μl|
|I tried the Tag antibody on the corresponding lysate but do not get any signal on a western blot. Why?|
Ensure the correct secondary antibody was used. Also, to get a good signal, try loading higher amounts of the lysate (such as 20ug) to strengthen the signal.
- Lloyd, T. E. et al. "Cytosolic 5′-Nucleotidase 1A As a Target of Circulating Autoantibodies in Autoimmune Diseases" Arthritis Care & Research 68(1): 66-71 (2015). DOI: 10.1002/acr.22600. Application: Cat. No. L032.