5X All-In-One RT MasterMix

G48525 x 10 µl reactions

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5X All-In-One RT MasterMix
5X All-In-One RT MasterMix

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    DescriptionFrom RNA template to cDNA in just 15 minutes completely hassle-free. With abm’s 5X All-In-One RT MasterMix, just add RNA and this optimized system will provide sensitive and reliable cDNA synthesis over a dynamic range of input RNA. The use of this MasterMix eliminates multiple component additions providing exceptional reproducibility and precision, in addition to offering the end-user ultimate convenience. Key Features • Reduction in handling errors with only 1 liquid transfer step • Streamlined protocol suitable for high-throughput applications • Simple set-up for any RNA template • High reproducibility and excellent yield Note - In case of no RT control, it is suggested to take a small aliquot of the 5X All-In-One RT MasterMix, and perform deactivation of the RT by incubating the small aliquot on heat block at 85°C for 5 -10 minutes prior to the reaction setup.
    Applications• Generation of templates for use in RT-PCR and qRT-PCR • cDNA synthesis from ssRNA • cDNA library construction • Generation of probes for hybridization • DNA primer extension
    Note This product is ISO-13485 certified.
    Storage ConditionStore all components at -20°C.
    Unit quantity25 x 10 µl reactions

    Can gene specific primers be used?
    Our 5X All-In-One RT MasterMix already contains oligo dT and random primers; gene specific primers cannot be used for this kit.
    What's the difference between the products of 15 and 50 min. cDNA synthesis, can I use the product of either of them to perform both PCR and qPCR?
    qPCR amplicons are usually very short a(e.g. 200bp) whereas PCR products are usually much larger and the unit is usually in terms of Kb. Therefore a longer time is required for PCR. Using 50mins for qPCR products is not recommended as you may get random products that skew your results (the reaction will reach a plateau after all your templates have been reverse transcribed and random products will be generated afterwards).
    What is the maximum length of cDNA synthesized by the 5X All-In-One RT MasterMix?
    G485 is able to synthesize cDNA up to around 10kb in length.
    Can G485 be used with miRNA without the PolyA tail?
    The PolyA tail must first be added to the miRNA, and thus we do not recommend using the 5X All-In-One RT MasterMix with your miRNA in its current state. Additionally, G485 contains random primers, therefore it is not recommended for this application.

    • Li, R et al. "Ultrafine particles from diesel vehicle emissions at different driving cycles induce differential vascular pro-inflammatory responses: implication of chemical components and NF-kappaB signaling" Part Fibre Toxicol 7:6 (2010). DOI: 10.1186/1743-8977-7-6. PubMed: 20307321. Application: Reverse Transcription.
    • Austin, K et al. "Insulin-like Growth Factor Binding Protein-4 is Required for the Growth Effects of Glucagon-like Peptide-2 In Murine Intestine." Endocrinology 156(2):429-436 (2014). PubMed: 25514089. Application: Reverse Transcription.
    • Zheng, H et al. "The quorum sensing regulator CinR hierarchically regulates two other quorum sensing pathways in ligand-dependent and -independent fashions in Rhizobium etli" J Bacteriol 197(9):1573-81 (2015). DOI: 10.1128/JB.00003-15. PubMed: 25691531. Application: Reverse Transcription.
    • Gagnon, J et al. "Ghrelin Is a Novel Regulator of GLP-1 Secretion" Diabetes 64(5):1513-21 (2015). DOI: 10.2337/db14-1176. PubMed: 25412624. Application: Reverse Transcription.
    • Gagnon, j et al. "Chronic Exposure to TNFα Impairs Secretion of Glucagon-Like Peptide-1" Endocrinology 156(11):3950-60 (2015). DOI: 10.1210/en.2015-1361. PubMed: 26270730. Application: Reverse Transcription.
    • Bian, W. P., Chen, Y. L., Luo, J. J., Wang, C., Xie, S. L., & Pei, D. S. "Knock-In Strategy for Editing Human and Zebrafish Mitochondrial DNA Using Mito-CRISPR/Cas9 System" ACS synthetic biology 8(4):621-632 (2019).
    • Biancolin, A. D., Martchenko, A., Mitova, E., Gurges, P., Michalchyshyn, E., Chalmers, J. A., ... & Gribble, F. M. "The core clock gene, Bmal1, and its downstream target, the SNARE regulatory protein secretagogin, are necessary for circadian secretion of glucagon-like peptide-1" Molecular Metabolism. : (2019).
    • Camunas-Soler, J., Dai, X., Hang, Y., Bautista, A., Lyon, J., Suzuki, K., … MacDonald, P. E. "Pancreas patch-seq links physiologic dysfunction in diabetes to single-cell transcriptomic phenotypes" : (2019). DOI: 10.1101/555110.
    • Chen, H., Yang, Y., Wang, Y., Li, Y., He, Y., Duan, J., … Li, Q. "Phospholipase C inhibits apoptosis of porcine primary granulosa cells cultured in vitro" Journal of Ovarian Research 12(1): (2019). DOI: 10.1186/s13048-019-0567-4.
    • Chen, M., Ye, K., Zhang, B., Xin, Q., Li, P., Kong, A.-N., … Yang, J. "Paris Saponin II inhibits colorectal carcinogenesis by regulating mitochondrial fission and NF-κB pathway" Pharmacological Research 139:273–285 (2019). DOI: 10.1016/j.phrs.2018.11.029.
    • Cui, C., Cheng, X., Yan, L., Ding, H., Guan, X., Zhang, W., … Hao, C. "

      Downregulation of TfR1 promotes progression of colorectal cancer via the JAK/STAT pathway

      " Cancer Management and Research Volume 11:6323–6341 (2019). DOI: 10.2147/cmar.s198911.
    • Deng, H., Gao, R., Chen, J., Liao, X., & Cai, Y. "An efficient polyethylene glycol-mediated transformation system of lentiviral vector in Shiraia bambusicola"  Process Biochemistry  51(10):1357-1362 (2016).
    • Dong, C., Wang, X., Li, N., Zhang, K., Wang, X., Zhang, H., ... & Ma, B. . "microRNA‑mediated GAS1 downregulation promotes the proliferation of synovial fibroblasts by PI3K‑Akt signaling in osteoarthritis" Experimental and Therapeutic Medicine : ().
    • Dong, D., Fan, T., Ji, Y., Yu, J., Wu, S., Zhang, L. "Spironolactone alleviates diabetic nephropathy through promoting autophagy in podocytes" International Urology and Nephrology 51:755-794 (2019). DOI: 10.1007/s11255-019-02074-9.
    • Dong., Pengzhen and Li., Huimin and Fu., Hao and Zhang., Shihui and Zhang., Xin and Chen., Yage and Li., Tao and Wu., Dongdong and Ji., Xinying.. "The PCNP/β-Catenin Axis Promotes Ovarian Cancer Progression" : (2019).
    • Duan, Q., Li, D., Xiong, L., Chang, Z., & Xu, G. "SILAC Quantitative Proteomics and Biochemical Analyses Reveal a Novel Molecular Mechanism by Which ADAM12S Promotes the Proliferation, Migration, and Invasion of Small Cell Lung Cancer Cells through Upregulating Hexokinase 1" Journal of Proteome Research 18(7):2903–2914 (2019). DOI: 10.1021/acs.jproteome.9b00208.
    • Feng, F., Wang, Z., Li, R., Wu, Q., Gu, C., Xu, Y., … He, H. "Citrus alkaline extracts prevent fibroblast senescence to ameliorate pulmonary fibrosis via activation of COX-2" Biomedicine & Pharmacotherapy 112:108669 (2019). DOI: 10.1016/j.biopha.2019.108669.
    • Grigorov, B., Reungoat, E., Gentil dit Maurin, A., Varbanov, M., Blaising, J., Michelet, M., … Pécheur, E.-I. "Hepatitis C virus infection propagates through interactions between Syndecan-1 and CD81 and impacts the hepatocyte glycocalyx" Cellular Microbiology 19(5):e12711 (2016). DOI: 10.1111/cmi.12711.
    • Heng, Y., Jiang, Y., Zhao, X., Zhou, H., Wang, X., Deng, X. W., & Xu, D. "BBX4, a phyB-interacting and modulated regulator, directly interacts with PIF3 to fine tune red light-mediated photomorphogenesis" Proceedings of the National Academy of Sciences. : (2019).
    • Heng, Y., Lin, F., Jiang, Y., Ding, M., Yan, T., Lan, H., … Deng, X. W. "B-Box Containing Proteins BBX30 and BBX31, Acting Downstream of HY5, Negatively Regulate Photomorphogenesis in Arabidopsis" Plant Physiology 180(1):497–508 (2019). DOI: 10.1104/pp.18.01244.
    • Hu, N., Wang, C., Dai, X., Zhou, M., Gong, L., Yu, L., … Li, Y. "Phillygenin inhibits LPS-induced activation and inflammation of LX2 cells by TLR4/MyD88/NF-κB signaling pathway" Journal of Ethnopharmacology 112361: (2019). DOI: 10.1016/j.jep.2019.112361.
    • Hu, T., Chong, Y., Cai, B., Liu, Y., Lu, S., & Cowell, J. K. "DNA methyltransferase 1-mediated CpG methylation of the miR-150-5p promoter contributes to fibroblast growth factor receptor 1-driven leukemogenesis" Journal of Biological Chemistry jbc-RA119: (2019).
    • Jing, Y., Liu, J., Liu, P., Ming, D., & Sun, J. "Overexpression of TaJAZ1 increases powdery mildew resistance through promoting reactive oxygen species accumulation in bread wheat" Scientific reports 9(1):5691 (2019).
    • Li, E., Zhang, T., Sun, X., Li, Y., Geng, H., Yu, D., & Zhong, C. "Sonic hedgehog pathway mediates genistein inhibition of renal cancer stem cells" Oncology Letters. : (2019). DOI: 10.3892/ol.2019.10657.
    • Li, P., Liu, A., Liu, C., Qu, Z., Xiao, W., Huang, J., ... & Zhang, S. "Role and mechanism of catechin in skeletal muscle cell differentiation" The Journal of nutritional biochemistry 74:108225 (2019).
    • Li, W., Zhang, Y., Han, B., Li, L., Li, M., Lu, X., … Zhang, B. "One-step efficient generation of dual-function conditional knockout and geno-tagging alleles in zebrafish" eLife 8: (2019). DOI: 10.7554/elife.48081.
    • Li, Z., Shen, J., & Liang, J. "Genome-Wide Identification, Expression Profile, and Alternative Splicing Analysis of the Brassinosteroid-Signaling Kinase (BSK) Family Genes in Arabidopsis"  International journal of molecular sciences  20(5):1138 (2019).
    • Lian, G., Li, X., Zhang, L., Zhang, Y., Sun, L., Zhang, X., … Jiang, C. "Macrophage metabolic reprogramming aggravates aortic dissection through the HIF1α-ADAM17 pathway" EBioMedicine. : (2019). DOI: 10.1016/j.ebiom.2019.09.041.
    • Liu, J., Luo, S., Yang, J., Ren, F., Zhao, Y., Luo, H., … Zhang, H. "The Protective Effect of Sheep Placental Extract on Concanavalin A-induced Liver Injury in Mice" Molecules 24(1):28 (2018). DOI: 10.3390/molecules24010028.
    • Ma, B., Zhang, J., Zhu, Z., Zhao, A., Zhou, Y., Ying, H., & Zhang, Q. "Luteolin Ameliorates Testis Injury and Blood–Testis Barrier Disruption through the Nrf2 Signaling Pathway and by Upregulating Cx43" Molecular nutrition & food research 1800843: (2019).
    • Millen, S., Gross, C., Donhauser, N., Mann, M. C., Jr., J.-M. P., & Thoma-Kress, A. K. "Collagen IV (COL4A1, COL4A2), a Component of the Viral Biofilm, Is Induced by the HTLV-1 Oncoprotein Tax and Impacts Virus Transmission" Frontiers in Microbiology 10: (2019). DOI: 10.3389/fmicb.2019.02439.
    • Qian, Y., Lynch, J. H., Guo, L., Rhodes, D., Morgan, J. A., & Dudareva, N. "Completion of the cytosolic post-chorismate phenylalanine biosynthetic pathway in plants" Nature Communications 10(1): (2019). DOI: 10.1038/s41467-018-07969-2.
    • Sha, J.-chuan, Jia, Z.-hang, Zhang, X., Wu, X.-xian, Ge, S.-feng, & Jiang, Y.-mao. "Effects of exogenous ABA on translocation of photosynthate to fruit of Fuji apple during late stage of fruit rapid-swelling" Yingyong Shengtai Xuebao 30(6):1854–1860 (2019).
    • Shan, W., Li, J., Xu, W., Li, H., & Zuo, Z. "Critical role of UQCRC1 in embryo survival, brain ischemic tolerance and normal cognition in mice" Cellular and Molecular Life Sciences 76(7):1381–1396 (2019). DOI: 10.1007/s00018-019-03007-6.
    • Shi, J., Fei, X., Hu, Y., Liu, Y., & Wei, A. "Identification of Key Genes in the Synthesis Pathway of Volatile Terpenoids in Fruit of Zanthoxylum bungeanum Maxim" Forests 10(4):328 (2019). DOI: 10.3390/f10040328.
    • Slim, G.M., Lansing, M., Wizzard, P., Nation, P.N., Wheeler, S.E., Brubaker, P.L., Jeppesen, P.B., Wales, P.W., Turner, J.M. "Novel long-acting GLP-2 analogue, FE 203799 (Apraglutide), Enhances Adaptation and Linear Intestinal Growth in a Neonatal Piglet Model of Short Bowel Syndrome with Total Resection of the Ileum" J. Parenteral and Enteral Nutrition 43(7):891-898 (2019). DOI: 10.1002/jpen.1500.
    • Tian, X., Yan, L., Zhang, D., Guan, X., Dong, B., Zhao, M., & Hao, C. "PTK7 overexpression in colorectal tumors: Clinicopathological correlation and prognosis relevance"  Oncology reports  36(4):1829-1836 (2016).
    • Wang, H., Li, P., Du, T., Pu, G., Fan, L., Gao, C., ... & Huang, R. "Effects of Increasing Levels of Defatted Rice Bran on Intestinal Physical Barrier and Bacteria in Finishing Pigs" Animals 9(12):1039 (2019).
    • Wang, H., Xu, L., Yu, M., Wang, Y., Jiang, T., Yang, S., & Lv, Z. "Glycosaminoglycan from Apostichopus japonicus induces immunomodulatory activity in cyclophosphamide-treated mice and in macrophages" International Journal of Biological Macromolecules 130:229–237 (2019). DOI: 10.1016/j.ijbiomac.2019.02.093.
    • Wang, J., Liu, S., Liu, H., Chen, K., & Zhang, P. "PnSAG1, an E3 ubiquitin ligase of the Antarctic moss Pohlia nutans, enhanced sensitivity to salt stress and ABA" Plant Physiology and Biochemistry 141:343–352 (2019). DOI: 10.1016/j.plaphy.2019.06.002.
    • Wang, W., Gao, T., Chen, J., Yang, J., Huang, H., & Yu, Y. "The late embryogenesis abundant gene family in tea plant (Camellia sinensis): Genome-wide characterization and expression analysis in response to cold and dehydration stress" Plant Physiology and Biochemistry 135:277–286 (2019). DOI: 10.1016/j.plaphy.2018.12.009.
    • Wang, X., Huo, H., Luo, Y., Liu, D., Zhao, L., Zong, L., … Wei, G. "Type III secretion systems impact Mesorhizobium amorphae CCNWGS0123 compatibility with Robinia pseudoacacia" Tree Physiology 39(9):1533–1550 (2019). DOI: 10.1093/treephys/tpz077.
    • Wei, F., Jing, Y.-Z., He, Y., Tang, Y.-Y., Yang, L.-T., Wu, Y.-F., … Xiong, F. "Cloning and characterization of the putative AFAP1-AS1 promoter region" Journal of Cancer 10(5):1145–1153 (2019). DOI: 10.7150/jca.29049.
    • Wen, M. H., Xie, X., Tub, J., Lee, D. F., & Chen, T. Y. "Generation of a genetically modified human embryonic stem cells expressing fluorescence tagged ATOX1" Stem Cell Research 101631: (2019).
    • Wen, M.-H., Xie, X., Tub, J., Lee, D.-F., & Chen, T.-Y. "Generation of a genetically modified human embryonic stem cells expressing fluorescence tagged ATOX1" Stem Cell Research 101631: (2019). DOI: 10.1016/j.scr.2019.101631.
    • Wu, Z., Zhang, W., Wu, Y., Wang, T., Wu, S., Wang, M., … Cheng, A. "Binding of the Duck Tembusu Virus Protease to STING Is Mediated by NS2B and Is Crucial for STING Cleavage and for Impaired Induction of IFN-β" The Journal of Immunology. : (2019). DOI: 10.4049/jimmunol.1900956.
    • Xiao, G., Jin, L. L., Liu, C. Q., Wang, Y. C., Meng, Y. M., Zhou, Z. G., ... & Zheng, L. "EZH2 negatively regulates PD-L1 expression in hepatocellular carcinoma" Journal for immunotherapy of cancer 7(1):1-15 (2019).
    • Xie, W., Xiao, J., Wang, T., Zhang, D., & Li, Z. "MicroRNA‐876‐5p inhibits cell proliferation, migration and invasion by targeting c‐Met in osteosarcoma" Journal of Cellular and Molecular Medicine 23(5):3293–3301 (2019). DOI: 10.1111/jcmm.14217.
    • Xu, W., Xu, Z., Huang, L., Qin, E.-Q., Zhang, J., Zhao, P., … Chen, W.-W. "Transcriptome Sequencing Identifies Novel Immune Response Genes Highly Related to the Severity of Human Adenovirus Type 55 Infection" Frontiers in Microbiology 10: (2019). DOI: 10.3389/fmicb.2019.00130.
    • Yang, F.W., Fang, B., Pang, G.F., Ren, F.Z. "Organophosphorus pesticide triazophos: A new endocrine disruptor chemical of hypothalamus-pituitary-adrenal axis" Pesticide Biochemistry and Physiology 159:91-97 (2019). DOI: 10.1016/j.pestbp.2019.05.021.
    • Yang, J., Dang, G., Lü, S., Liu, H., Ma, X., Han, L., ... & Jiang, C. "T-cell–derived extracellular vesicles regulate B-cell IgG production via pyruvate kinase muscle isozyme 2" The FASEB Journal 33(11):12780-12799 (2019).
    • Yang, S., Deng, Q., Sun, L., Dong, K., Li, Y., Wu, S., & Huang, R. "Salmonella effector SpvB interferes with intracellular iron homeostasis via regulation of transcription factor NRF2" The FASEB Journal fj-201900883RR: (2019).
    • Yao, X., Yu, T., Xi, F., Xu, Y., Ma, L., Pan, X., … Xie, L. "BAMBI shuttling between cytosol and membrane is required for skeletal muscle development and regeneration" Biochemical and Biophysical Research Communications 509(1):125–132 (2019). DOI: 10.1016/j.bbrc.2018.12.082.
    • Zhang, J., Bao, X., Zhang, M., Zhu, Z., Zhou, L., Chen, Q., … Ma, B. "MitoQ ameliorates testis injury from oxidative attack by repairing mitochondria and promoting the Keap1-Nrf2 pathway" Toxicology and Applied Pharmacology 370:78–92 (2019). DOI: 10.1016/j.taap.2019.03.001.
    • Zhao, L., Wang, L., Zhang, C. "E6-induced selective translation of WNT4 and JIP2 promotes the progression of cervical cancer via a noncanonical WNT signaling pathway" Sig Transduct Target Ther 4(32) : (2019). DOI: 10.1038/s41392-019-0060-y.
    • Zhao, Q., Wang, Q., Wang, J., Tang, M., Huang, S., Peng, K., … You, Z. "Maternal immune activation-induced PPARγ-dependent dysfunction of microglia associated with neurogenic impairment and aberrant postnatal behaviors in offspring" Neurobiology of Disease 125:1–13 (2019). DOI: 10.1016/j.nbd.2019.01.005.