5X Protein Loading Buffer

Cat. No.
G031
Unit
3.0 ml
Price
$52.50
Cat. No. G031
Name 5X Protein Loading Buffer
Unit 3.0 ml
Category Gels, Blots & ELISAs
Description

Simplify and standardize your sample loading with abm’s 5X Protein Loading Buffer. This pre-mixed and ready-to-use reducing sample buffer is provided in a convenient 5X formulation. Mix one part sample buffer with four parts protein sample, heat denature and then load the sample in the gel wells. Visualize progress of the electrophoresis run by monitoring the location of the blue dye front. Protein Loading Buffer may be used in denaturing gels of all kinds and are compatible with coomassie dye and silver staining techniques, as well as Western blotting procedures.

Composition:  Tris-HCl pH 6.8, 10% w/v SDS, 250 mM DTT, 0.03% w/v bromophenol blue, and 50% v/v glycerol

Storage Condition

Store at -20°C for long-term storage or at 4°C for short-term storage.

Material Citation If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. G031
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  • Sharma, V et al. "Registered report: COT drives resistance to RAF inhibition through MAP kinase pathway reactivation" eLIFE 5:e11414 (2016). DOI: 10.7554/eLife.11414. Application: Western Blot.
  • Kim, S., Lee, M. S., Jung, S., Son, H. Y., Park, S., Kang, B., ... & Kim, Y. "Ginger Extract Ameliorates Obesity and Inflammation via Regulating MicroRNA-21/132 Expression and AMPK Activation in White Adipose Tissue"  Nutrients  10(11):1567 (2018).
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