Bst DNA Polymerase, Large Fragment

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Bst DNA Polymerase, Large Fragment
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Cat.No.: E039
Quantity: 1600 U (200 μl)
Price: $55.00
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Data Sheet
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Description Bst DNA Polymerase, Large Fragment is a DNA polymerase from Bacillus stearothermophilus that is modified so that it retains its 5’→ 3’ DNA polymerase activity while lacking the intrinsic 5’→ 3’ exonuclease domain. This polymerase also has strand displacement capabilities making it an ideal candidate for isothermal amplification.
Application • Isothermal amplification
• DNA sequencing of high GC DNA
• Sequencing from very low amounts of template DNA
• DNA strand displacement amplification
Form Enzyme supplied with 2X Reaction Buffer
Image
Concentration 8 U/μl
Storage Store all components at -20°C.
Storage Buffer 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA, 1 mM DTT, 50 mM KCl, 0.1% Triton® X-100 and 50% (v/v) Glycerol.
Notes One unit is defined as the amount of Bst DNA Polymerase, Large Fragment that that catalyzes the incorporation of 10 nmol of dNTP into acid insoluble material in 30 minutes at 65°C.

This product is distributed for laboratory research only
Caution: Not for diagnostic use.
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