CRISPR Bacterial Genome Editing Service
CRISPR has rapidly become the genome editing tool of choice in many eukaryote organisms, however its use in bacteria has been limited until recently. Many bacteria do not possess robust DNA repair systems as eukaryotes do, and therefore CRISPR-induced double-stranded DNA breaks are lethal. To improve recombination rates scientists have employed phage-derived (Lambda Red) recombinases to carry out enhanced homologous recombination – referred to as recombineering. When Lambda Red recombination is coupled with CRISPR it provides a powerful tool for selecting against non-edited cells, allowing for highly precise and efficient scarless genome editing.
Bacteria possess great potential to produce complex molecules with applications in therapeutics, agriculture, and industry. E. coli has long been a preferred choice for metabolic engineering and the production of such molecules due to its well-characterization and abundance of available genetic tools. Our custom bacterial gene knockout and knock-in services offer highly specific and efficient editing of the E. coli genome.
When the locus of your knock-in isn’t a concern, consider our E. coli Safe Harbor Gene Knock-In Service. Our expert scientists have verified this “safe harbor” or “hotspot” locus in the E. coli genome for safe guarded genomic expression. Use this service for reliable expression of your gene of interest. To order bacterial genome editing services, simply send us the information on your desired gene knock-in/knockout and E. coli strain, and we’ll do the rest!
- CRISPR-assisted gene knockout: Partial or whole gene deletion of your choice.
- CRISPR-assisted gene knock-in: Tag an existing gene or knock-in a gene of interest.
- Tried-and-true genome editing from expert scientists: see our Bacterial CRISPR Case Studies for more information.
- Validated Safe Harbor locus allows for stable, single copy genomic expression of your gene or construct of interest.
|E. coli Gene Knockout Service||Deliverables||Lead Time||Cat. No.||Price|
||Sequence verified engineered strain in glycerol stock (2 different clones)||8 weeks||C424||$3950.00|
|E. coli Gene Knock-in Service||Deliverables||Lead Time||Cat. No.||Price|
||Sequence verified engineered strain in glycerol stock (2 different clones)||9 weeks||C425||$5000.00|
|E. coli Safe Harbor Gene Knock-in Service||Deliverables||Lead Time||Cat. No.||Price|
||Sequence verified engineered strain at the safe harbor locus in a glycerol stock (2 different clones)||9 weeks||C520||$4495.00|
|Add‑On Services||Unit||Cat. No.||Price|
|Additional Clones||1 Clone||C143||$500.00|
|Off-Target Analysis by Whole Genome Sequencing
* Includes sequencing and analysis of edited vs. unedited strains
Please note that if the gene to be knocked-out may be essential to cell survival, it is up to the end-user to proceed with the services. abm is unable to guarantee cell survival in these cases and will only attempt to rescue the clones under these conditions. abm is not accountable for cell survival if rescuing the clones (instructions to be provided customers) is unsuccessful.
*** A deposit is required to initiate all CRISPR Bacterial Gene Editing projects
|How should I submit E. coli cells for this service?|
Using sterile technique, grow the specific strain overnight without selection, ensuring that it is turbid and dense the next day. In an appropriate cryotube vial, mix the overnight dense culture with glycerol to a final concentration of 15% (for a total of volume of at least 1ml). Freeze at -80°C, until ready to ship on dry ice to abm.