CRISPR Bacterial Genome Editing Service

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Service Deliverables Lead Time Cat. No. Price
E. coli Gene Knockout Service Sequence verified engineered strain in glycerol stock (2 different clones) 8 weeks C424 $3950
E. coli Gene Knock-in Service Sequence verified engineered strain in glycerol stock (2 different clones) 9 weeks C425 $5000
 
Service Details

CRISPR has rapidly become the genome editing tool of choice in many eukaryote organisms, however its use in bacteria has been limited until recently. Many bacteria do not possess robust DNA repair systems as eukaryotes do, and therefore CRISPR-induced double-stranded DNA breaks are lethal. To improve recombination rates scientists have employed phage-derived (Lambda Red) recombinases to carry out enhanced homologous recombination – referred to as recombineering. When Lambda Red recombination is coupled with CRISPR it provides a powerful tool for selecting against non-edited cells, allowing for highly precise and efficient scarless genome editing.

Bacteria possess great potential to produce complex molecules with applications in therapeutics, agriculture, and industry. E. coli has long been a preferred choice for metabolic engineering and the production of such molecules due to its well-characterization and abundance of available genetic tools. Our custom bacterial gene knockout and knock-in services offer highly specific and efficient editing of the E. coli genome. Simply send us the information on your desired gene knock-in/knockout and E. coli strain, and we’ll do the rest!

E. coli Gene Knockout Service Deliverables Lead Time Cat. No. Price
1. Select E. coli strain
2. sgRNA vector design/construction (3+ sgRNAs)
3. Gene Knockout
4. Clonal screening and selection
5. Validation of genomic edit by diagnostic PCR and Sanger sequencing
Sequence verified engineered strain in glycerol stock (2 different clones) 8 weeks C424 $3950

E. coli Gene Knock-in Service Deliverables Lead Time Cat. No. Price
1. Select E. coli strain
2. sgRNA vector design/construction (3+ sgRNAs) and donor DNA construction
3. Gene Knock-in
4. Clonal screening and selection
5. Validation of genomic edit by diagnostic PCR and Sanger sequencing
Sequence verified engineered strain in glycerol stock (2 different clones) 9 weeks C425 $5000

Add-on Services^ Quantity Cat. No. Price
Off Target Analysis by Whole Genome Sequencing* 1 Service C426 $1695.00
Additional Clones 1 Clone C143 $500.00

* Includes sequencing and analysis of edited vs. unedited strains
*** A deposit is required to initiate all CRISPR Bacterial Gene Editing projects

^All “add on” services need to be indicated prior to order placement as the project design will need to be finalized prior to start of the service.

Please note that if the gene to be knocked-out may be essential to cell survival, it is up to the end-user to proceed with the services. abm is unable to guarantee cell survival in these cases and will only attempt to rescue the clones under these conditions. abm is not accountable for cell survival if rescuing the clones (instructions to be provided customers) is unsuccessful.

CRISPR Bacterial Genome Editing Workflow
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