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    Unit: 1x106 cells / 1.0 ml
    To generate the Cas9 Expressing Immortalized Chicken Embryonic Fibroblast (DF1) Cell Line, Cas9-2A-PuroR was cloned into pT2KCAGGS using Gibson Assembly (NEB). The DF1 cells were then transfected, and selected for using puromycin. The constitutive...


  2. Price: $395.00
    Unit: 10 µg
    This construct consists of the catalytic domain of Ten-Eleven Translocation dioxygenase 1 (TET1CD) fused to the C-terminus of Cas9 Double Mutant (dCas9). The Cas9 Double Mutant has changes at amino acid positions D10A and H840A which completely...


  3. Price: $395.00
    Unit: 10 µg
    This construct consists of the catalytic domain of DNA methyltransferase (DNMT3A) fused to the C-terminus of Cas9 Double Mutant (dCas9). The Cas9 Double Mutant has changes at amino acid positions D10A and H840A which completely inactivate both...


  4. Price: $695.00
    Unit: 300 µl
    This construct consists of the catalytic domain of DNA methyltransferase (DNMT3A) fused to the C-terminus of Cas9 Double Mutant (dCas9). The Cas9 Double Mutant has changes at amino acid positions D10A and H840A which completely inactivate both...


  5. Price: $695.00
    Unit: 300 µl
    This construct consists of the catalytic domain of Ten-Eleven Translocation dioxygenase 1 (TET1CD) fused to the C-terminus of Cas9 Double Mutant (dCas9). The Cas9 Double Mutant has changes at amino acid positions D10A and H840A which completely...


  6. Price: $50.00
    Unit: 6.5µg (50pmol/ 50µL)
    The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 system is the latest RNA-guided endonuclease tool in genome editing which allows for very specific genomic disruption and replacement. The Cas9 nuclease serves to unwind the...


  7. Price: $145.00
    Unit: 32.5µg (250pmol/ 50µL)
    The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 system is the latest RNA-guided endonuclease tool in genome editing which allows for very specific genomic disruption and replacement. The Cas9 nuclease serves to unwind the...


  8. Price: $250.00
    Unit: 25 Reactions
    The use of Cpf1 (a.k.a. Cas12a) in CRISPR is a relatively recent discovery, and the science of crRNA design is still developing. As such, crRNA efficiency should always be verified before use in further experiments such as cell transfection or...


  9. Price: $250.00
    Unit: 25 Reactions
    The use of Cpf1 (a.k.a. Cas12a) in CRISPR is a relatively recent discovery, and the science of crRNA design is still developing. As such, crRNA efficiency should always be verified before use in further experiments such as cell transfection or...


  10. Price: $350.00
    Unit: 380 μg (2.5 nmol/ 250µL)
    Using Cpf1 (a.k.a. Cas12a) in your CRISPR experiment offers several advantages over other CRISPR-associated nucleases. Due to the T-rich PAM sequence (TTTN), Cpf1 enables editing in regions unable to be targeted by Cas9. Cpf1 can be used with a...

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