CRISPR Gene Editing Tools

Cas9 Expressing Immortalized Chicken Embryonic Fibroblast (DF1) Cell Line
Cas9-Expressing Cell Lines

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Sku: T3453
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Unit: 1x10⁶ cells / 1.0 ml
To generate the Cas9 Expressing Immortalized Chicken Embryonic Fibroblast (DF1) Cell Line, Cas9-2A-PuroR was cloned into pT2KCAGGS using Gibson Assembly (NEB). The DF1 cells were then transfected, and selected for using puromycin. The constitutive...

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dCas9-TET1CD Lentiviral Vector
Cas9 Expression Vectors and Viruses

Sku: K096
Price: $395.00
Unit: 10 µg
This construct consists of the catalytic domain of Ten-Eleven Translocation dioxygenase 1 (TET1CD) fused to the C-terminus of Cas9 Double Mutant (dCas9). The Cas9 Double Mutant has changes at amino acid positions D10A and H840A which completely...
dCas9-DNMT3A Lentiviral Vector
Cas9 Expression Vectors and Viruses

Sku: K091
Price: $395.00
Unit: 10 µg
This construct consists of the catalytic domain of DNA methyltransferase (DNMT3A) fused to the C-terminus of Cas9 Double Mutant (dCas9). The Cas9 Double Mutant has changes at amino acid positions D10A and H840A which completely inactivate both...
dCas9-DNMT3A Lentivirus
Cas9 Expression Vectors and Viruses

Sku: K092
Price: $695.00
Unit: 300 µl
This construct consists of the catalytic domain of DNA methyltransferase (DNMT3A) fused to the C-terminus of Cas9 Double Mutant (dCas9). The Cas9 Double Mutant has changes at amino acid positions D10A and H840A which completely inactivate both...
dCas9-TET1CD Lentivirus
Cas9 Expression Vectors and Viruses

Sku: K090
Price: $695.00
Unit: 300 µl
This construct consists of the catalytic domain of Ten-Eleven Translocation dioxygenase 1 (TET1CD) fused to the C-terminus of Cas9 Double Mutant (dCas9). The Cas9 Double Mutant has changes at amino acid positions D10A and H840A which completely...
saCas9 Nuclease NLS Protein
Cas9 Proteins

Sku: K089
Price: $50.00
Unit: 6.5µg (50pmol/ 50µL)
The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 system is the latest RNA-guided endonuclease tool in genome editing which allows for very specific genomic disruption and replacement. The Cas9 nuclease serves to unwind the...
saCas9 Nuclease NLS Protein (High Concentration)
Cas9 Proteins

Sku: K189
Price: $145.00
Unit: 32.5µg (250pmol/ 50µL)
The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 system is the latest RNA-guided endonuclease tool in genome editing which allows for very specific genomic disruption and replacement. The Cas9 nuclease serves to unwind the...
GeneCraft-R asCpf1 crRNA Screening Kit
GeneCraft-R CRISPR Kits

Sku: G966
Price: $250.00
Unit: 25 Reactions
The use of Cpf1 (a.k.a. Cas12a) in CRISPR is a relatively recent discovery, and the science of crRNA design is still developing. As such, crRNA efficiency should always be verified before use in further experiments such as cell transfection or...
GeneCraft-R fnCpf1 crRNA Screening Kit
GeneCraft-R CRISPR Kits

Sku: G965
Price: $250.00
Unit: 25 Reactions
The use of Cpf1 (a.k.a. Cas12a) in CRISPR is a relatively recent discovery, and the science of crRNA design is still developing. As such, crRNA efficiency should always be verified before use in further experiments such as cell transfection or...
asCpf1 Nuclease NLS Protein (2.5 nmol)
Cas9 Proteins

Sku: K188
Price: $350.00
Unit: 380 μg (2.5 nmol/ 250µL)
Using Cpf1 (a.k.a. Cas12a) in your CRISPR experiment offers several advantages over other CRISPR-associated nucleases. Due to the T-rich PAM sequence (TTTN), Cpf1 enables editing in regions unable to be targeted by Cas9. Cpf1 can be used with a...