Cell Immortalization Kits

Primary cells have a finite life span of less than 10-50 passages in vitro depending on cell type. Immortalizing your cell line will eliminate the frequent need to re-establish fresh cultures from specimens. Immortalized cell lines are capable of extended proliferation and should possess similar or identical genotype and phenotype to the parental tissue.

Immortalization Strategies for Mammalian Cells
Several methods exist for immortalizing mammalian cells in culture. With years of experience, abm has developed the most comprehensive cell immortalization products comprising of ready-to-use retroviral, lentiviral and adenoviral vectors.

All Lentiviruses listed below are provided at 109 IU/ml titer, 2 x 100 μl volume, and adenoviruses listed below are provided at 106 pfu/ml, 1 ml volume.

TERT Overexpression Viruses

May cause cell death in some cell types (eg. epithelial cells)

Product Name Cat. No. Vector Map Promoter Marker Price
Lentivirus
Lenti-hTERT Virus LV615 View CMV Puro Inquire
Lenti-hTERT-Neo Virus LV622 View CMV Neo Inquire
Lenti-hTERT (No Selection Marker) virus LV674 View CMV   Inquire
Lenti-hTERT-2A-CDK4 Virus LV677 View CMV Puro Inquire
Lenti-EF1a-hTERT Virus LV616 View EF1a Puro Inquire
Lenti-EF1a-hTERT-Hygro Virus LV632 View EF1a Hygro Inquire
Adenovirus
Adeno-hTERT Virus G205 View CMV   Inquire
Adeno-hTERT Antisense Virus G208 View CMV   Inquire

SV40T Antigen Overexpression Viruses

Often used to immortalize epithelial cells

Product Name Cat. No. Vector Map Promoter Marker Price
Lentivirus
Lenti-SV40T (Puro) Lentivirus LV613 View CMV Puro Inquire
Lenti-SV40T (Neo) Lentivirus LV660 View CMV Neo Inquire
Lenti-SV40T (No Selection Marker) Lentivirus LV665 View CMV   Inquire
Lenti-SV40Tt Lentivirus LV614 View CMV Puro Inquire
Lenti-SV40 Lentivirus LV612 View CMV   Inquire
Lenti-SV40T (tsA58 temp sensitive) Lentivirus LV630 View CMV Puro Inquire
Lenti-SV40T (tsA58 temp sensitive) (No Selection Marker) Lentivirus LV680 View
CMV Inquire
Adenovirus
Adeno-SV40 Adenovirus G210 View CMV Puro Inquire

Other Overexpression Viruses

Product Name Cat. No. Vector Map Application Price
HPV E6/E7
Lenti-HPV-16 E6/E7 (Puro) Virus LV617 View Immortalize keratinocytes Inquire
Lenti-HPV-16 E6/E7 (No Selection Marker) Virus LV667 View Inquire
EBV genes (Epstein-Barr Virus)
EBV Virus (Wild-Type) G229 N/A Immortalize B lymphocytes, and sometimes T lymphocytes Inquire
Adenoviral E1A-E1B
E1A-E1B Lentivirus LV652 View Immortalize a wide range of primary rodent cells, including amniotic fluid cells, epithelial cells, fibroblasts, and kidney cells Inquire
siRNA
Lenti-Rb siRNA Virus LV621 View Co-express with hTERT for better immortalization of epithelial cells Inquire
Lenti-p53 siRNA Virus LV619 View Inquire
Bmi1
Lenti-Bmi1 Virus LV608 View Immortalize epithelial cells Inquire
CDK4
Lenti-hTERT-2A-CDK4 Virus LV677 View Co-express with hTERT for a more authentic cell model Inquire
Lenti-CDK4 Virus LV609 View Inquire
Myc T58A (c-Myc), Ras V12 (H-Ras)
Lenti-Myc T58A Virus LV618 View Immortalizes a wide variety of cells, including neural stem cells, primary kidney cells, erythroblasts, and a variety of epithelial cells Inquire
Lenti-Ras V12 Virus LV620 View Inquire
HOXB8, HOXA9, HOXA10
Lenti-HOXB8-GFP Lentivirus LV637 View Immortalize hematopoietic cells, including macrophages, hematopoetic progenitor cells, and myeloid progenitor cells Inquire
Lenti-HOXB8-RFP Lentivirus LV638 View Inquire
Lenti-HOXB8-C-term-HA Lentivirus LV636 View Inquire
Lenti-HOXA9-GFP Lentivirus LV643 View Inquire
Lenti-HOXA9-RFP Lentivirus LV644 View Inquire
Lenti-HOXA9-C-term-HA Lentivirus LV642 View Inquire
Lenti-HOXA10-GFP Lentivirus LV649 View Inquire
Lenti-HOXA10-RFP Lentivirus LV650 View Inquire
Lenti-HOXA10-C-term-HA Lentivirus LV648 View Inquire
Other reagents for cell immortalization
ViralEntry™ Transduction Enhancer (100X) G515 Boosts lentiviral transduction Inquire

Tools & Resources

  • Cell Immortalization Handbook
    Cell Immortalization Handbook
    This Handbook is designed to give an overview of Cell Immortalization, Including methods, protocols, and reagents.
  • Cell Immortalization Workflow
    Cell Immortalization Workflow
    Overview of abm's Immortalization service workflow. For more information, visit our Cell Immortalization Knowledge base.
  • Custom Cell Immortalization Service
    Custom Cell Immortalization Service
    Can't find what you are looking for? We can help!