Custom Adeno-Associated Virus (AAV) Service
As a leading expert in AAV technology, abm offers a Custom AAV Service where we can help design, clone and/or viral package your custom AAV construct. We offer a range of AAV serotypes (1-11) as well as purifications including crude (ideal for in vitro experiments) and purified (optimal for in vivo experiments). Inquire with us below if you're looking for Custom Cloned AAV Vectors or Custom AAV Packaging.
abm also offers several ready-to-use AAV vector/virus collections:
- AAV Gene Expression
- AAV CRISPR Genome-wide Knockout
- AAV miRNA Expression
- AAV siRNA Genome-wide Knockdown
Advantages of the AAV System:
- Used as a promising candidate for gene therapy
- Does not elicit significant immune responses in vivo
- Broad tropism - tissue specificity with different AAV serotypes
- No integration into the host genome
- Ability to transduce both proliferating and quiescent cells
- Long-term expression in non-dividing cells
Our AAV infectivity Data Speaks for Itself
Left: EGFP expression (Green) in lumbar neuronal cells 4 weeks after intrathecal injection of AAV-EGFP Serotype 9 (Cat. # iAAV01509) into mice. Right: Overlay with β-tubulin (red) and DAPI (blue). Image courtesy of Dr. Douglas Lopes, King’s College London.
Additional Resources:
"If I have any special needs or concerns, they are always dealt with by the Technical Support Team. I have always been pleased with the product and will continue to use this service for my research."
John Teem, Florida Department of Agriculture & Consumer ServicesService Details
Expand for DetailsService Selection Assistant & Services List
How are you going to provide your insert?
What titer would you like to produce?
What serotype would you like to use?
Gene Synthesis
| Service Name | Size | CAT. NO. | PRICE |
|---|---|---|---|
| Gene Synthesis | Per bp | C098 | Starting at USD$0.18/bp |
*minimum charge $99; maximum size 3kb for AAV.
Custom AAV Plasmid Cloning
| Service Name | Size | CAT. NO. | PRICE |
|---|---|---|---|
| Custom AAV plasmid cloning with compatible insert | 1 Service | C234 | $350.00 |
| Custom AAV plasmid cloning without compatible insert | 1 Service | C235 | $450.00 |
*For these services, the customer must provide a gene insert under 3kb. Please contact [email protected] for more information on available cloning vectors and restriction enzyme sites to qualify for the compatible insert service.
Custom AAV Production without DNA Amplification
| Service Name | Size | CAT. NO. | PRICE |
|---|---|---|---|
| Custom AAV without DNA amplification (10^11 GC/ml) (AAV1) | 3 x 100 μl | C239 | $325.00 |
| Custom AAV without DNA amplification (10^11 GC/ml) (AAV2) | 3 x 100 μl | C240 | $325.00 |
| Custom AAV without DNA amplification (10^11 GC/ml) (AAV3) | 3 x 100 μl | C290 | $325.00 |
| Custom AAV without DNA amplification (10^11 GC/ml) (AAV4) | 3 x 100 μl | C291 | $325.00 |
| Custom AAV without DNA amplification (10^11 GC/ml) (AAV5) | 3 x 100 μl | C241 | $325.00 |
| Custom AAV without DNA amplification (10^11 GC/ml) (AAV6) | 3 x 100 μl | C242 | $325.00 |
| Custom AAV without DNA amplification (10^11 GC/ml) (AAV7) | 3 x 100 μl | C243 | $325.00 |
| Custom AAV without DNA amplification (10^11 GC/ml) (AAV8) | 3 x 100 μl | C244 | $325.00 |
| Custom AAV without DNA amplification (10^11 GC/ml) (AAV9) | 3 x 100 μl | C245 | $325.00 |
| Custom AAV without DNA amplification (10^11 GC/ml) (AAV10) | 3 x 100 μl | C246 | $325.00 |
| Custom AAV without DNA amplification (10^11 GC/ml) (AAV11) | 3 x 100 μl | C344 | $325.00 |
| Custom AAV without DNA amplification (10^12 GC/ml) (AAV1) | 5 x 200 μl | C247 | $895.00 |
| Custom AAV without DNA amplification (10^12 GC/ml) (AAV2) | 5 x 200 μl | C248 | $895.00 |
| Custom AAV without DNA amplification (10^12 GC/ml) (AAV3) | 5 x 200 μl | C292 | $895.00 |
| Custom AAV without DNA amplification (10^12 GC/ml) (AAV4) | 5 x 200 μl | C293 | $895.00 |
| Custom AAV without DNA amplification (10^12 GC/ml) (AAV5) | 5 x 200 μl | C249 | $895.00 |
| Custom AAV without DNA amplification (10^12 GC/ml) (AAV6) | 5 x 200 μl | C250 | $895.00 |
| Custom AAV without DNA amplification (10^12 GC/ml) (AAV7) | 5 x 200 μl | C251 | $895.00 |
| Custom AAV without DNA amplification (10^12 GC/ml) (AAV8) | 5 x 200 μl | C252 | $895.00 |
| Custom AAV without DNA amplification (10^12 GC/ml) (AAV9) | 5 x 200 μl | C253 | $895.00 |
| Custom AAV without DNA amplification (10^12 GC/ml) (AAV10) | 5 x 200 μl | C254 | $895.00 |
| Custom AAV without DNA amplification (10^12 GC/ml) (AAV11) | 5 x 200 μl | C488 | $895.00 |
| Custom AAV without DNA amplification (10^13 GC/ml) (AAV1) | 10 x 50 μl | C255 | $1695.00 |
| Custom AAV without DNA amplification (10^13 GC/ml) (AAV2) | 10 x 50 μl | C256 | $1695.00 |
| Custom AAV without DNA amplification (10^13 GC/ml) (AAV3) | 10 x 50 μl | C294 | $1695.00 |
| Custom AAV without DNA amplification (10^13 GC/ml) (AAV4) | 10 x 50 μl | C295 | $1695.00 |
| Custom AAV without DNA amplification (10^13 GC/ml) (AAV5) | 10 x 50 μl | C257 | $1695.00 |
| Custom AAV without DNA amplification (10^13 GC/ml) (AAV6) | 10 x 50 μl | C258 | $1695.00 |
| Custom AAV without DNA amplification (10^13 GC/ml) (AAV7) | 10 x 50 μl | C259 | $1695.00 |
| Custom AAV without DNA amplification (10^13 GC/ml) (AAV8) | 10 x 50 μl | C260 | $1695.00 |
| Custom AAV without DNA amplification (10^13 GC/ml) (AAV9) | 10 x 50 μl | C261 | $1695.00 |
| Custom AAV without DNA amplification (10^13 GC/ml) (AAV10) | 10 x 50 μl | C262 | $1695.00 |
| Custom AAV without DNA amplification (10^13 GC/ml) (AAV11) | 10 x 50 μl | C491 | $1695.00 |
To qualify for Custom AAV Production without DNA amplification, the following amounts of the AAV vector containing the gene of interest must be supplied at >0.5μg/μl:
- 10^11 GC/ml: 75 μg
- 10^12 GC/ml: 400 μg
- 10^13 GC/ml: 800 μg
Custom AAV Production with DNA Amplification
| Service Name | Size | CAT. NO. | PRICE |
|---|---|---|---|
| Custom AAV with DNA amplification (10^11 GC/ml) (AAV1) | 3 x 100 μl | C263 | $425.00 |
| Custom AAV with DNA amplification (10^11 GC/ml) (AAV2) | 3 x 100 μl | C264 | $425.00 |
| Custom AAV with DNA amplification (10^11 GC/ml) (AAV3) | 3 x 100 μl | C296 | $425.00 |
| Custom AAV with DNA amplification (10^11 GC/ml) (AAV4) | 3 x 100 μl | C297 | $425.00 |
| Custom AAV with DNA amplification (10^11 GC/ml) (AAV5) | 3 x 100 μl | C265 | $425.00 |
| Custom AAV with DNA amplification (10^11 GC/ml) (AAV6) | 3 x 100 μl | C266 | $425.00 |
| Custom AAV with DNA amplification (10^11 GC/ml) (AAV7) | 3 x 100 μl | C267 | $425.00 |
| Custom AAV with DNA amplification (10^11 GC/ml) (AAV8) | 3 x 100 μl | C268 | $425.00 |
| Custom AAV with DNA amplification (10^11 GC/ml) (AAV9) | 3 x 100 μl | C269 | $425.00 |
| Custom AAV with DNA amplification (10^11 GC/ml) (AAV10) | 3 x 100 μl | C270 | $425.00 |
| Custom AAV with DNA amplification (10^11 GC/ml) (AAV11) | 3 x 100 μl | C347 | $425.00 |
| Custom AAV with DNA amplification (10^12 GC/ml) (AAV1) | 5 x 200 μl | C271 | $995.00 |
| Custom AAV with DNA amplification (10^12 GC/ml) (AAV2) | 5 x 200 μl | C272 | $995.00 |
| Custom AAV with DNA amplification (10^12 GC/ml) (AAV3) | 5 x 200 μl | C298 | $995.00 |
| Custom AAV with DNA amplification (10^12 GC/ml) (AAV4) | 5 x 200 μl | C299 | $995.00 |
| Custom AAV with DNA amplification (10^12 GC/ml) (AAV5) | 5 x 200 μl | C273 | $995.00 |
| Custom AAV with DNA amplification (10^12 GC/ml) (AAV6) | 5 x 200 μl | C274 | $995.00 |
| Custom AAV with DNA amplification (10^12 GC/ml) (AAV7) | 5 x 200 μl | C275 | $995.00 |
| Custom AAV with DNA amplification (10^12 GC/ml) (AAV8) | 5 x 200 μl | C276 | $995.00 |
| Custom AAV with DNA amplification (10^12 GC/ml) (AAV9) | 5 x 200 μl | C277 | $995.00 |
| Custom AAV with DNA amplification (10^12 GC/ml) (AAV10) | 5 x 200 μl | C278 | $995.00 |
| Custom AAV with DNA amplification (10^12 GC/ml) (AAV11) | 5 x 200 μl | C494 | $995.00 |
| Custom AAV with DNA amplification (10^13 GC/ml) (AAV1) | 10 x 50 μl | C279 | $1795.00 |
| Custom AAV with DNA amplification (10^13 GC/ml) (AAV2) | 10 x 50 μl | C280 | $1795.00 |
| Custom AAV with DNA amplification (10^13 GC/ml) (AAV3) | 10 x 50 μl | C300 | $1795.00 |
| Custom AAV with DNA amplification (10^13 GC/ml) (AAV4) | 10 x 50 μl | C301 | $1795.00 |
| Custom AAV with DNA amplification (10^13 GC/ml) (AAV5) | 10 x 50 μl | C281 | $1795.00 |
| Custom AAV with DNA amplification (10^13 GC/ml) (AAV6) | 10 x 50 μl | C282 | $1795.00 |
| Custom AAV with DNA amplification (10^13 GC/ml) (AAV7) | 10 x 50 μl | C283 | $1795.00 |
| Custom AAV with DNA amplification (10^13 GC/ml) (AAV8) | 10 x 50 μl | C284 | $1795.00 |
| Custom AAV with DNA amplification (10^13 GC/ml) (AAV9) | 10 x 50 μl | C285 | $1795.00 |
| Custom AAV with DNA amplification (10^13 GC/ml) (AAV10) | 10 x 50 μl | C286 | $1795.00 |
| Custom AAV with DNA amplification (10^13 GC/ml) (AAV11) | 10 x 50 μl | C497 | $1795.00 |
Custom siRNA AAV Production
| Service Name | Size | CAT. NO. | PRICE |
|---|---|---|---|
| AAV siRNA Pooled Virus (AAV1)(10^11 GC/ml) | 3 x 100 μl | iAAV04401 | $670.00 |
| AAV siRNA Pooled Virus (AAV2)(10^11 GC/ml) | 3 x 100 μl | iAAV04402 | $670.00 |
| AAV siRNA Pooled Virus (AAV3)(10^11 GC/ml) | 3 x 100 μl | iAAV04403 | $670.00 |
| AAV siRNA Pooled Virus (AAV4)(10^11 GC/ml) | 3 x 100 μl | iAAV04404 | $670.00 |
| AAV siRNA Pooled Virus (AAV5)(10^11 GC/ml) | 3 x 100 μl | iAAV04405 | $670.00 |
| AAV siRNA Pooled Virus (AAV6)(10^11 GC/ml) | 3 x 100 μl | iAAV04406 | $670.00 |
| AAV siRNA Pooled Virus (AAV7)(10^11 GC/ml) | 3 x 100 μl | iAAV04407 | $670.00 |
| AAV siRNA Pooled Virus (AAV8)(10^11 GC/ml) | 3 x 100 μl | iAAV04408 | $670.00 |
| AAV siRNA Pooled Virus (AAV9)(10^11 GC/ml) | 3 x 100 μl | iAAV04409 | $670.00 |
| AAV siRNA Pooled Virus (AAV10)(10^11 GC/ml) | 3 x 100 μl | iAAV04410 | $670.00 |
| AAV siRNA Pooled Virus (AAV11)(10^11 GC/ml) | 3 x 100 μl | C500 | $670.00 |
| Custom AAV siRNA Vector (Set of 4) | 4 x 1.0 μg | iAAV04400 | $395.00 |
Custom AAV Titration
| Service Name | Size | CAT. NO. | PRICE |
|---|---|---|---|
| Custom AAV Titration using qPCR Assay | 1 Service | C238 | $85.00 |
AAV Virus Custom Aliquoting
| Service Name | Size | CAT. NO. | PRICE |
|---|---|---|---|
| AAV Virus Custom Aliquoting* | Up to 10 Vials | C506 | $50.00 |
Additional Info
Don't Know Which Serotype To Use?
Related Products
Documents
FAQs
| Is your recombinant AAV replication-deficient? |
|
Yes, the replication and capsid genes are provided in trans when the AAV is produced, therefore the packaged virion only has the ITR sequences and the gene of interest. Furthermore, the cis plasmid and rep/cap plasmid do not share any regions of homology, preventing the production of wild-type AAV through recombination system.
|
| What is the packaging capacity for AAV? |
|
The maximum insert size is < 4.4 kb.
|
| Is AAV stable? What is the recommended storage temperature? |
|
It is recommended to store AAV at -80˚C for long-term storage. For short-term, AAV is stable at 4˚C for up to three weeks without significant loss of activity.
|
| How much AAV virus should be submitted for the Custom AAV Titration service, Cat# C238? |
|
Please send at least 50μl of virus if it is low titer. If it is expected to be high titer AAV virus, please send at least 20μl of sample.
|
Citations
| 01 | Zhang, Y. et al. "Hypothalamic stem cells control ageing speed partly through exosomal miRNAs." Nature. 548(7665):52-57 (2017). DOI: 10.1038/nature23282. PubMed: 28746310. Application: In vivo infection. |
| 02 | Papes, F. et al. "Transcription Factor 4 loss-of-function is associated with deficits in progenitor proliferation and cortical neuron content." Nature Communications. 13:2387. (2022). DOI: 10.1038/s41467-022-29942-w. PubMed: 35501322. |
| 03 | Li, J. et al. "Activation of the chemokine receptor CCR1 and preferential recruitment of Gαi suppress RSV replication: implications for developing novel RSV treatment strategies." Journal of Virology. 96(22):e0130922. (2022). DOI: 10.1128/jvi.01309-22. PubMed: 36317881 |
| 04 | Scano, M. et al. "CFTR corrector C17 is effective in muscular dystrophy, in vivo proof of concept in LGMDR3." Human Molecular Genetics. 31(4):499-509. (2022). DOI: 10.1093/hmg/ddab260. PubMed: 34505136 |