Custom Cloning Service

A one-stop source for research solutions, abm offers gene synthesis, cloning, and virus packaging services aimed to reduce the time, effort and money spent by researchers.

abm's top-notch custom services include, but are not limited to:

subcloning
gene synthesis
codon optimization (upon request)
mutagenesis
expression vector customization:
tissue-specific promoters Examples of tissue-specific promoters:
hSyn
TBG
GFAP promoter
CD14 promoter
B29 promoter
Endoglin promoter
Desmin promoter
CamKIIα
Albumin
; fluorescent reporters; tags and lox sites; drug resistance markers
sequence verification of inserts in final vector; vector verification by restriction digest

Our experienced scientists will assemble your clone accurately, rapidly, economically, and ready to use for your experiment.

"Of all the companies I have reached out to, abm has been the best in terms of explaining the technology and how it fits my application."

Tim Erickson, Ingenovax, Custom Cloning Service

Additional Resources:

Service Details

Core Services

Service Name	Description	Unit	Cat. No.	Price
Economy Gene Synthesis & Subcloning	For non-complicated sequences Synthesize up to 950 bp Complimentary subcloning into pUC57, EcoRV site Sequence verification to confirm full length sequence is generated Sequence must be approved for economy synthesis by abm	1.0 μg	C078	$250.00
Gene Synthesis	For any sequence up to 4 kbp in size Genes are fully sequenced Must be ordered with Subcloning Service I (C096) Minimum charge of USD$99.00	1.0 μg	C098	Starting at USD$0.18/bp*
Subcloning Service I	Subclone any insert into an expression system of your choice. This service is intended for inserts with restriction sites that are compatible with the expression vector of choice. End sequencing of the insert is performed with this service to confirm proper insertion. For full sequencing, please refer to C197.	1.0 μg	C096	$125.00
Subcloning Service II	Subclone any insert into an expression system of your choice. This service is intended for inserts that are not compatible with restriction sites of the expression vector of choice. Full length DNA sequencing is performed for sequence verification. Genes over 3 kbp are subject to an additional charge of $100.00/kbp (C100).	1.0 μg	C097	$225.00
Site-directed Mutagenesis	Mutate up to 25 bp (consecutive) within a < 2 kbp fragment in any existing abm product or a provided vector. Full length sequencing to confirm presence of mutation Fragments over 2 kbp are subject to an additional charge of $100.00/kbp (C100).	1.0 μg	C047	$150.00
Additional Charge for Extra 1 kbp	Additional charge for fragments over the stated size limit ($100.00/kbp) Only applicable to C097, C047 and C128.	N/A	C100	$100.00
Promoter Change Service	Change the promoter in one of abm’s vectors or a vector of your choice to any of the following: CMV, EF1α, PGK, UbC	1.0 μg	C126	$350.00
Tag Addition/Removal Service (≤ 30 bp)	Addition/Removal of an N-terminal or C-terminal tag smaller than 30 bp	1.0 μg	C127	$250.00
Tag Addition/Removal Service (> 30 bp) *	Addition/Removal of an N-terminal or C-terminal tag larger than 30 bp Tags over 1 kbp are subject to an additional charge of $100.00/kbp (C100).	1.0 μg	C128	$350.00
Reporter Addition/Removal Service	Addition, removal or switching of a reporter (e.g. GFP, RFP, Luc) in one of abm’s vectors or a vector of your choice.	1.0 μg	C129	$350.00
Antibiotic Switching Service	Switching of a Puromycin resistance gene to Neomycin, Hygromycin or Blasticidin resistance in one of abm’s vectors or a vector of your choice.	1.0 μg	C140	$350.00

ADD-ON-SERVICES

Service Name	Description	Unit	Cat. No.	Price
DNA Sequencing	Verify the sequence and position of your gene of interest or sequence your PCR products.	Single Reaction	C199	$7.50
Bacterial Agar Stab	Bacterial stab of the custom plasmid.	1 clone	C315	$40.00
Plasmid Amplification (10-20 μg)	Amplification of the custom plasmid.	10-20 μg	C309	$75.00/prep
Plasmid Amplification (80-100 μg)	Amplification of the custom plasmid.	80-100 μg	C310	$150.00/prep

***Bulk amplification services available here

Additional Info

abm Vectors Available for Use in the Above Services
Cloning into pUC57 or pORF is free of charge; for use of other abm vector backbones the charge is $350 (Cat. No. C318). Here is a selected list of expression vectors. Cloning into retrovirus, HSV, baculovirus, and much more is also offered at abm.

Lentiviral System	AAV System	Adenoviral System	Protein Expression System
pLenti-III-HA	pAAV-G-CMV-amp	pShuttle (+)	pPB-C-His
pLenti-EF1a-GFP-2A-Puro	pAAV-G-EF1a-hGH-amp	pShuttle (-)	pPB-His-MBP
pLenti-CMV-GFP-2A-Puro	pAAV-G-PGK-hGH-amp	pShuttle-HA	pPB-His GST
pLenti-CMV-RFP-2A-Puro	pAAV-G-CAGGS-hGH-amp	pShuttle-His	pPB-N-His
pLenti-Bi-cistronic		pPromoter	pPM-C-His
pLenti-Tri-cistronic		pPM-C-HA
pLenti-III-EF1α		pPM-N-D-C-HA
pLenti-III-PGK		pPM-N-D-C-His
pLenti-III-UbC
pLenti-Promoterless
Additional lentiviral vectors available.	Additional vectors available.	Additional vectors available.

Viral packaging services available upon request.
We can also insert your gene in an inverse orientation, flanked by double lox sites, so that it will only flip for expression in cells that express Cre recombinase.
Try tissue-specific promoters for controlled gene expression.

Documents

FAQs

For the Antibiotic Switching Service, can I change the gene to a resistance marker other than Puromycin or Neomycin?

Yes, we can certainly look into this for you. Please email [email protected] for further information.

Citations

01	Wen, Z et al. "MicroRNA-377 Regulates Mesenchymal Stem Cell-Induced Angiogenesis in Ischemic Hearts by Targeting V." PLoS One 9(9):e104666 (2014). DOI: 10.1371/journal.pone.0104666 PubMed: 25251394 Application: Inhibitor miRNA lentivector.
02	Laryea, G et al. "Site-specific modulation of brain glucocorticoid receptor and corticotropin-releasing hormone expression using lentiviral vectors." Mol Cell Endocrinol 371(1-2):160-5 (2013). DOI: 23261985 PubMed: 10.1016/j.mce.2012.12.005 Application: EF1a lentivector.
03	Das, A et al. "Engulfment of apoptotic cells by macrophages: a role of microRNA-21 in the resolution of wound inflammation.." J Immunol 192(3):1120-9 (2014). DOI: 10.4049/jimmunol.1300613 PubMed: 24391209 Application: Transduction.
04	Eagleman, DE et al." Unbiased proteomic screening identifies a novel role for the E3 ubiquitin ligase Nedd4-2 in translational suppression during ER stress." J Neurochem 157(6):1809-1820 (2021). DOI: 10.1111/jnc.15219 PubMed: 33064840 Application: Site Directed Mutagenesis.