Custom Cloning Service

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To order custom services please contact us.
Service Description Cat. No. Quant. Price
Economy Gene Synthesis & Subcloning For non-complicated sequences
Synthesize up to 950bp
- Complimentary subcloning into pUC57, EcoRV site
- Sequence verification to confirm full length sequence is generated
- Sequence must be approved for economy synthesis by abm		 C078 1.0 μg $250.00
Gene Synthesis For any sequence up to 4kb in size
Genes are fully sequenced
Must be ordered with Subcloning Service I (C096)
*minimum charge of $99.00		 C098 1.0 μg $0.18/bp USD
Subcloning Service I Subclone any insert into an expression system of your choice. This service is intended for inserts with restriction sites that are compatible with the expression vector of choice.
End sequencing of the insert is included with this service to confirm proper insertion*.
*For full sequencing, please refer to C197.		 C096 1.0 μg $125.00
Subcloning Service II Subclone any insert into an expression system of your choice. This service is intended for inserts with restriction sites that are not compatible with the expression vector of choice.
Full length DNA sequencing is included for sequence verification.
*Genes over 3kb are subject to an additional charge of $100.00/kb (C100).		 C097 1.0 μg $225.00
Site-directed Mutagenesis Mutate up to 25bp (consecutive) within a <2kb fragment in any existing abm product or a provided vector.
Full length sequencing to confirm presence of mutation
*Fragments over 2kb are subject to an additional charge of $100.00/kb (C100).		 C047 1.0 μg $150.00*
Additional Charge
for Extra 1kb		 Additional charge for fragments over the stated size limit ($100.00/kb)
*Only applicable to C097 and C047.		 C100 N/A $100.00
Promoter Change Service Change the promoter in one of abm’s vectors or a vector of your choice to any of the following: CMV, EF1a, PGK, UbC C126 1.0 μg $350.00
Tag Addition/Removal Service (≤30bp) Addition/Removal of an N-terminal or C-terminal tag smaller than 30bp C127 1.0 μg $250.00
Tag Addition/Removal Service (>30bp) Addition/Removal of an N-terminal or C-terminal tag larger than 30bp
*Tags over 1kb are subject to an additional charge of $100.00/kb (C100).		 C128 1.0 μg $350.00
Reporter Addition/Removal Service Addition, removal or switching of a GFP or RFP reporter in one of abm’s vectors or a vector of your choice. C129 1.0 μg $350.00
Antibiotic Switching Service Switching of a Puromycin resistance gene to Neomycin, Hygromycin or Blasticidin resistance in one of abm’s vectors or a vector of your choice. C140 1.0 μg $350.00
DNA Sequencing Verify the sequence and position of your gene of interest or sequence your PCR products. C199 Single Reaction $7.50/rxn
DNA Sequencing with Custom Primer Synthesis Verify the sequence and position of your gene of interest or sequence your PCR products. C197 Single Reaction $13.50/rxn
Bacterial Glycerol Stock Bacterial culture of the custom plasmid. C139 1 Clone $25.00
Plasmid Amplification (10-20 μg) Amplification of the custom plasmid. C309 10-20 μg $75.00/rxn
Plasmid Amplification (80-100 μg) Amplification of the custom plasmid. C310 80-100 μg $150.00/rxn
Service Details
Description
  A one-stop source for research solutions: abm offers a comprehensive selection of life-sciences related custom services aimed to reduce the time, effort and money spent by researchers.

  abm's premium custom services include, but are not limited to:
  • subcloning
  • gene synthesis
  • mutagenesis
  • gene fusion
  • optimized expression vectors (i.e. adding reporters, tags or promoters)

abm's technological capability and expertise not only ensure 'peace of mind' in the quality of the final product, but also rapid turnaround times and the flexibility to service individual needs based on specific research projects.

Order Form for Custom Cloning Service:
Key Features
  • Convenient one-stop shopping that can be tailored to the researcher’s experimental needs.
  • Vectors are verified via restriction digest and all synthesized inserts are 100% sequence verified within our in house sequencing facility (C098, C097, C047).
  • Further downstream applications (i.e. virus production, stable cell line generation) are also conveniently available at abm.
abm Vectors Available for Use in the Above Services
You can choose pUC57 or pORF to be used free of charge, or any of the below vectors with a charge of $350.00 per vector (Cat.No. C318). The base vector will not be a deliverable. Only the final altered vector will be provided.
Lentiviral System  Adenoviral System  Protein Expression System
pLenti-III-HA  pShuttle (+)  pPM-His
pLenti-EF1a-GFP-2A-Puro  pShuttle (-)  pPM-HA
pLenti-CMV-GFP-2A-Puro  pShuttle-HA  pPB-His
pLenti-CMV-RFP-2A-Puro  pShuttle-His  pPB-His-MBP
pLenti-Bi-cistronic  pPromoter  pPB-His GST
pLenti-Tri-cistronic    pPM-N-D-C-HA
pLenti-III-EF1α    pPM-N-D-C-His
pLenti-III-PGK    
pLenti-III-UbC    
pLenti-Promoterless    
Additional lentiviral vectors available. Additional vectors available. * Vector maps and catalog numbers coming soon. Please contact customer service for more information regarding the above vectors.
Custom cloning involving the lentivectors can be packaged into lentiviruses. Custom cloning involving these vectors can be packaged into adenoviruses.
Deliverables
You will receive 1-2ug of DNA containing custom construct, sequence chromatogram trace files, gene report, QC restriction digestion files, an alignment file as well as vector information.
Product Documents
Product References
FAQs
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For the Antibiotic Switching Service, can I change the gene to a resistance marker other than Puromycin or Neomycin?
If you would like other resistance markers, we would need the customer to provide the plasmid containing the marker, as well as the full sequence, and we can then look into details of the marker and see if it would be possible. Please email technical@abmgood.com for further information.