Custom Cloning Service

A one-stop source for research solutions, abm offers gene synthesis, cloning, and virus packaging services aimed to reduce the time, effort and money spent by researchers.

abm's top-notch custom services include, but are not limited to:

  • subcloning
  • gene synthesis
  • codon optimization (upon request)
  • mutagenesis
  • expression vector customization:
    tissue-specific promotersExamples of tissue-specific promoters:
    hSyn
    TBG
    GFAP promoter
    CD14 promoter
    B29 promoter
    Endoglin promoter
    Desmin promoter
    CamKIIα
    Albumin
    ; fluorescent reporters; tags and lox sites; drug resistance markers
  • sequence verification of inserts in final vector; vector verification by restriction digest

Our experienced scientists will assemble your clone accurately, rapidly, economically, and ready to use for your experiment.



"Of all the companies I have reached out to, abm has been the best in terms of explaining the technology and how it fits my application."

Tim Erickson, Ingenovax, Custom Cloning Service

Additional Resources:

Service Details

Core Services
Service NameDescriptionUnitCat. No.Price
Economy Gene Synthesis & Subcloning
  • For non-complicated sequences
  • Synthesize up to 950 bp
  • Complimentary subcloning into pUC57, EcoRV site
  • Sequence verification to confirm full length sequence is generated
  • Sequence must be approved for economy synthesis by abm
1.0 μg C078 $250.00
Gene Synthesis
  • For any sequence up to 4 kbp in size
  • Genes are fully sequenced
  • Must be ordered with Subcloning Service I (C096)
  • Minimum charge of USD$99.00
1.0 μg C098 Starting at USD$0.18/bp*
Subcloning Service I
  • Subclone any insert into an expression system of your choice. This service is intended for inserts with restriction sites that are compatible with the expression vector of choice.
  • End sequencing of the insert is performed with this service to confirm proper insertion.
  • For full sequencing, please refer to C197.
1.0 μg C096 $125.00
Subcloning Service II
  • Subclone any insert into an expression system of your choice. This service is intended for inserts that are not compatible with restriction sites of the expression vector of choice.
  • Full length DNA sequencing is performed for sequence verification.
  • Genes over 3 kbp are subject to an additional charge of $100.00/kbp (C100).
1.0 μg C097 $225.00
Site-directed Mutagenesis
  • Mutate up to 25 bp (consecutive) within a < 2 kbp fragment in any existing abm product or a provided vector.
  • Full length sequencing to confirm presence of mutation
  • Fragments over 2 kbp are subject to an additional charge of $100.00/kbp (C100).
1.0 μg C047 $150.00
Additional Charge for Extra 1 kbp
  • Additional charge for fragments over the stated size limit ($100.00/kbp)
  • Only applicable to C097, C047 and C128.
N/A C100 $70.00
Promoter Change Service
  • Change the promoter in one of abm’s vectors or a vector of your choice to any of the following: CMV, EF1α, PGK, UbC
1.0 μg C126 $350.00
Tag Addition/Removal Service (≤ 30 bp)
  • Addition/Removal of an N-terminal or C-terminal tag smaller than 30 bp
1.0 μg C127 $250.00
Tag Addition/Removal Service (> 30 bp) *
  • Addition/Removal of an N-terminal or C-terminal tag larger than 30 bp
  • Tags over 1 kbp are subject to an additional charge of $100.00/kbp (C100).
1.0 μg C128 $350.00
Reporter Addition/Removal Service
  • Addition, removal or switching of a reporter (e.g. GFP, RFP, Luc) in one of abm’s vectors or a vector of your choice.
1.0 μg C129 $350.00
Antibiotic Switching Service
  • Switching of a Puromycin resistance gene to Neomycin, Hygromycin or Blasticidin resistance in one of abm’s vectors or a vector of your choice.
1.0 μg C140 $350.00
ADD-ON-SERVICES
Service NameDescriptionUnitCat. No.Price
DNA Sequencing
  • Verify the sequence and position of your gene of interest or sequence your PCR products.
Single Reaction C199 $7.50
Bacterial Agar Stab
  • Bacterial stab of the custom plasmid.
1 clone C315 $40.00
Plasmid Amplification (10-20 μg)
  • Amplification of the custom plasmid.
10-20 μg C309 $75.00/prep
Plasmid Amplification (80-100 μg)
  • Amplification of the custom plasmid.
80-100 μg C310 $150.00/prep
***Bulk amplification services available here

Additional Info

abm Vectors Available for Use in the Above Services
Cloning into pUC57 or pORF is free of charge; for use of other abm vector backbones the charge is $350 (Cat. No. C318). Here is a selected list of expression vectors. Cloning into retrovirus, HSV, baculovirus, and much more is also offered at abm.

Lentiviral SystemAAV SystemAdenoviral SystemProtein Expression System
pLenti-III-HA pAAV-G-CMV pShuttle (+) pPB-C-His
pLenti-EF1a-GFP-2A-Puro pAAV-G-EF1a pShuttle (-) pPB-His-MBP
pLenti-CMV-GFP-2A-Puro pAAV-G-PGK pShuttle-HA pPB-His GST
pLenti-CMV-RFP-2A-Puro pAAV-G-CAGGS pShuttle-His pPB-N-His
pLenti-Bi-cistronic pAAV-G-MSCV pPromoter pPM-C-His
pLenti-Tri-cistronic pPM-C-HA
pLenti-III-EF1α pPM-N-D-C-HA
pLenti-III-PGK pPM-N-D-C-His
pLenti-III-UbC
pLenti-Promoterless
Additional lentiviral vectors available. Additional vectors available. Additional vectors available.
  • Viral packaging services available upon request.
  • We can also insert your gene in an inverse orientation, flanked by double lox sites, so that it will only flip for expression in cells that express Cre recombinase.
  • Try tissue-specific promoters for controlled gene expression.

FAQs

For the Antibiotic Switching Service, can I change the gene to a resistance marker other than Puromycin or Neomycin?
Yes, we can certainly look into this for you. Please email [email protected] for further information.

Citations

Coming Soon