Custom Cloning Service
A one-stop source for research solutions, abm offers gene synthesis, cloning, and virus packaging services aimed to reduce the time, effort and money spent by researchers.
abm's top-notch custom services include, but are not limited to:
- subcloning
- gene synthesis
- codon optimization (upon request)
- mutagenesis
- expression vector customization:
tissue-specific promoters Examples of tissue-specific promoters:; fluorescent reporters; tags and lox sites; drug resistance markers
hSyn
TBG
GFAP promoter
CD14 promoter
B29 promoter
Endoglin promoter
Desmin promoter
CamKIIα
Albumin - sequence verification of inserts in final vector; vector verification by restriction digest
Our experienced scientists will assemble your clone accurately, rapidly, economically, and ready to use for your experiment.
"Of all the companies I have reached out to, abm has been the best in terms of explaining the technology and how it fits my application."
Tim Erickson, Ingenovax, Custom Cloning ServiceAdditional Resources:
Service Details
Core Services
Service Name | Description | Unit | Cat. No. | Price |
---|---|---|---|---|
Economy Gene Synthesis & Subcloning |
|
1.0 μg | C078 | $250.00 |
Gene Synthesis |
|
1.0 μg | C098 | Starting at USD$0.18/bp* |
Subcloning Service I |
|
1.0 μg | C096 | $125.00 |
Subcloning Service II |
|
1.0 μg | C097 | $225.00 |
Site-directed Mutagenesis |
|
1.0 μg | C047 | $150.00 |
Additional Charge for Extra 1 kbp |
|
N/A | C100 | $100.00 |
Promoter Change Service |
|
1.0 μg | C126 | $350.00 |
Tag Addition/Removal Service (≤ 30 bp) |
|
1.0 μg | C127 | $250.00 |
Tag Addition/Removal Service (> 30 bp) * |
|
1.0 μg | C128 | $350.00 |
Reporter Addition/Removal Service |
|
1.0 μg | C129 | $350.00 |
Antibiotic Switching Service |
|
1.0 μg | C140 | $350.00 |
ADD-ON-SERVICES
Service Name | Description | Unit | Cat. No. | Price |
---|---|---|---|---|
DNA Sequencing |
|
Single Reaction | C199 | $7.50 |
Bacterial Agar Stab |
|
1 clone | C315 | $40.00 |
Plasmid Amplification (10-20 μg) |
|
10-20 μg | C309 | $75.00/prep |
Plasmid Amplification (80-100 μg) |
|
80-100 μg | C310 | $150.00/prep |
Additional Info
abm Vectors Available for Use in the Above ServicesCloning into pUC57 or pORF is free of charge; for use of other abm vector backbones the charge is $350 (Cat. No. C318). Here is a selected list of expression vectors. Cloning into retrovirus, HSV, baculovirus, and much more is also offered at abm.
Lentiviral System | AAV System | Adenoviral System | Protein Expression System |
---|---|---|---|
pLenti-III-HA | pAAV-G-CMV-amp | pShuttle (+) | pPB-C-His |
pLenti-EF1a-GFP-2A-Puro | pAAV-G-EF1a-hGH-amp | pShuttle (-) | pPB-His-MBP |
pLenti-CMV-GFP-2A-Puro | pAAV-G-PGK-hGH-amp | pShuttle-HA | pPB-His GST |
pLenti-CMV-RFP-2A-Puro | pAAV-G-CAGGS-hGH-amp | pShuttle-His | pPB-N-His |
pLenti-Bi-cistronic | pPromoter | pPM-C-His | |
pLenti-Tri-cistronic | pPM-C-HA | ||
pLenti-III-EF1α | pPM-N-D-C-HA | ||
pLenti-III-PGK | pPM-N-D-C-His | ||
pLenti-III-UbC | |||
pLenti-Promoterless | |||
Additional lentiviral vectors available. | Additional vectors available. | Additional vectors available. |
- Viral packaging services available upon request.
- We can also insert your gene in an inverse orientation, flanked by double lox sites, so that it will only flip for expression in cells that express Cre recombinase.
- Try tissue-specific promoters for controlled gene expression.
Related Products
FAQs
For the Antibiotic Switching Service, can I change the gene to a resistance marker other than Puromycin or Neomycin? |
Yes, we can certainly look into this for you. Please email [email protected] for further information.
|
Citations
01 | Wen, Z et al. "MicroRNA-377 Regulates Mesenchymal Stem Cell-Induced Angiogenesis in Ischemic Hearts by Targeting V." PLoS One 9(9):e104666 (2014). DOI: 10.1371/journal.pone.0104666 PubMed: 25251394 Application: Inhibitor miRNA lentivector. |
02 | Laryea, G et al. "Site-specific modulation of brain glucocorticoid receptor and corticotropin-releasing hormone expression using lentiviral vectors." Mol Cell Endocrinol 371(1-2):160-5 (2013). DOI: 23261985 PubMed: 10.1016/j.mce.2012.12.005 Application: EF1a lentivector. |
03 | Das, A et al. "Engulfment of apoptotic cells by macrophages: a role of microRNA-21 in the resolution of wound inflammation.." J Immunol 192(3):1120-9 (2014). DOI: 10.4049/jimmunol.1300613 PubMed: 24391209 Application: Transduction. |
04 | Eagleman, DE et al." Unbiased proteomic screening identifies a novel role for the E3 ubiquitin ligase Nedd4-2 in translational suppression during ER stress." J Neurochem 157(6):1809-1820 (2021). DOI: 10.1111/jnc.15219 PubMed: 33064840 Application: Site Directed Mutagenesis. |