At ABM we have a breadth of experience in purifying plasmid DNA. As a result, we offer high quality custom nucleic acid and plasmid DNA purification services of Transfection Grade for mammalian cells or Ready-to-Inject for transgenic applications. Our plasmid purification services use the E. coli
DH5α strain to produce optimal quality for downstream cell culture applications. Please contact firstname.lastname@example.org
if you wish to use other strains for your project demands and we can create a custom quotation.
All customer information is held in strict confidence. All materials and information sent to us and the products produced by us for the order are the property of the customer and will be returned to the customer or discarded in an confidential manner. We only archive customer materials when instructed to.
I want to learn more about your ready to inject BAC purification services. I have 2 BAC clones that need to be isolated, digested (linearized), and purified for pronuclear injection in mice.
Though the BAC is much larger than regular plasmid, we can still offer this service as long as the BAC is under 15kb. For BAC service, a special quotation has to be offered, depending on the nature of a particular plasmid.
The digestion will cost another $100~200/100ug depending which enzymes to be used as some enzymes are more expensive than others.
How much plasmid do I need to send you? How do you want it to be sent?
Please send in at least 1 μg of plasmid DNA or your transformed bacteria. Information for the host bacteria is required if you are sending transformed bacteria with your plasmids. Also, please provide the antibiotic selection marker if available.
What sort of information do I need to provide for the service?
1. Number of plasmid.
2. Amount of DNA for each plasmid.
3. Plasmid map to check for transformation strain compatibility.
4. Antibiotic for proper plasmid selection.
5. Insert name so it can be confirmed to not be toxic or pathogenic.
6. High or low copy plasmid.
Can I send my plasmid as bacterial stocks?
Typically, we request to receive the plasmids in liquid format as purified DNA, since this is most stable. However, bacterial stocks would also be suitable, provided that they are shipped to us within 2-3 days of preparation to ensure that the bacteria retain the plasmid.