Custom Retrovirus Services
abm’s custom retroviruses are a highly-efficient method to achieve stable gene expression in a broad range of dividing mammalian cells. Simply send us your gene, or purchase an ORF vector from our extensive library of human, mouse, or rat genes, and we will do the rest! Our packaged retroviruses are available with your choice of no tag, HA tag, or GFP reporter.
KEY FEATURES OF abm's CUSTOM RETROVIRUS SERVICE | |
---|---|
Promoter | CMV promoter for strong gene expression in most cellular systems |
Tags Available | No tag HA tag for detection or protein purification GFP reporter |
Safety | Replication-incompetent retrovirus with minimal relation to the wild-type Moloney Murine Leukemia Virus (MoMuLV) |
Additional Resources:
"abm has always been very helpful with technical assistance in any of our questions and concerns. I also consider the price for the services provided as very competitive, as other companies charge a multiple of abm's price."
Dr. Cameron Moshfegh, ETH ZurichService Details
Custom Retroviral Vectors
Service Name | Description | Unit | CAT. NO. | PRICE |
---|---|---|---|---|
Custom Retroviral Vector using ORF Library with No Tag | Any human, mouse, or rat gene from our extensive ORF library, available in a retroviral vector with no tag. Corresponding ORF vector must be ordered with this service. Maximum insert size that can be packaged is 4.5 kb. |
1.0 μg | C362 | $150.00 |
Custom Retroviral Vector using ORF Library with HA Tag | Any human, mouse, or rat gene from our extensive ORF library, available in a retroviral vector with a C-term HA tag. Corresponding ORF vector must be ordered with this service. Maximum insert size that can be packaged is 4.5 kb. |
1.0 μg | C363 | $300.00 |
Custom Retroviral Vector using ORF Library with GFP Reporter | Any human, mouse, or rat gene from our extensive ORF library, available in a retroviral vector with a GFP reporter. Corresponding ORF vector must be ordered with this service. Maximum insert size that can be packaged is 3.2 kb. |
1.0 μg | C364 | $300.00 |
Custom Retroviral Vector | Custom retrovirus plasmid subcloning. Provide your DNA and we’ll subclone it into a retroviral vector. | 1 Service | C057 | $550.00 |
Gene Synthesis | For any sequence up to 4.5 kb in size. Genes are fully-sequenced. Must be ordered with Custom Retroviral Vector (C362, C363 or C364). * Minimum charge of $99.00 |
1.0 μg | C098 | $0.18/bp USD |
Custom Retroviruses
Service Name | Quant. DNA Provided | CAT. NO. | Quant | PRICE | |
---|---|---|---|---|---|
Custom Retrovirus | Enough DNA Provided | ≥50 μg | C510 | 1 ml at 107 IU/ml | $600 |
DNA Amplification Required | <50 μg | C517 | 1 ml at 107 IU/ml | $700 |
Retrovirus Controls
Product | Unit | CAT. NO. | PRICE |
---|---|---|---|
pRetroG-CMV-blank Vector | 1.0 μg | RV001 | $135.00 |
pRetroG-CMV-GFP-HA Vector | 1.0 μg | RV002 | $135.00 |
pRetroG-CMV-GFP-stop Vector | 1.0 μg | RV003 | $135.00 |
pRetroG-CMV-GFP-IRES-GFP Vector | 1.0 μg | RV004 | $135.00 |
pRetroG-CMV-RFP-stop Vector | 1.0 μg | RV005 | $135.00 |
Retro-CMV Blank Retrovirus | 1 ml of 106 IU/ml | RVP001 | $195.00 |
Retro-CMV-GFP-HA Retrovirus | 1 ml of 106 IU/ml | RVP002 | $195.00 |
Retro-CMV-GFP Retrovirus | 1 ml of 106 IU/ml | RVP003 | $195.00 |
Retro-CMV-GFP-IRES-GFP Retrovirus | 1 ml of 106 IU/ml | RVP004 | $195.00 |
Retro-CMV-RFP Retrovirus | 1 ml of 106 IU/ml | RVP005 | $195.00 |
Retrovirus Vectors (click for details):
Additional Info
Related Products
FAQs
How long does it take to produce virus? |
This will be dependent on a number of factors, including the plasmid you would like to package. Please send all inquiries to [email protected] for a prompt response on the estimated lead time.
|
How do you determine titer? |
Via qPCR
|
What is the packaging capacity for Retrovirus? |
The maximum insert size is <8 kb between the 5'LTR to 3'LTR.
|
What is the minimum amount of DNA needed for subcloning into a retroviral vector (C517)? |
The minimum amount of DNA we require is 10μg for subcloning into a retroviral vector.
|
Citations
01 | Thomas, A. M. et al. "Polysaccharide-modified scaffolds for controlled lentivirus delivery in vitro and after spinal cord injury." J Control Release. 3:421-429 (2013). DOI: 10.1016/j.jconrel.2013.06.013 PubMed: 23791981 |