Custom Retrovirus Services

abm’s custom retroviruses are a highly-efficient method to achieve stable gene expression in a broad range of dividing mammalian cells. Simply send us your gene, or purchase an ORF vector from our extensive library of human, mouse, or rat genes, and we will do the rest! Our packaged retroviruses are available with your choice of no tag, HA tag, or GFP reporter.

KEY FEATURES OF ABM'S CUSTOM RETROVIRUS SERVICE
Promoter CMV promoter for strong gene expression in most cellular systems
Tags Available No tag
HA tag for detection or protein purification
GFP reporter
Retovirus TIters Up to 109 IU/ml
Safety Replication-incompetent retrovirus with minimal relation to the wild-type Moloney Murine Leukemia Virus (MoMuLV)




"abm has always been very helpful with technical assistance in any of our questions and concerns. I also consider the price for the services provided as very competitive, as other companies charge a multiple of abm's price."

Dr. Cameron Moshfegh, ETH Zurich

Additional Resources:

Service Details

Custom Retroviral Vectors
Service NameDescriptionUnitCAT. NO.PRICE
Custom Retroviral Vector using ORF Library with No Tag Any human, mouse, or rat gene from our extensive ORF library, available in a retroviral vector with no tag.
Corresponding ORF vector must be ordered with this service. Maximum insert size that can be packaged is 4.5 kb.
1.0 μg C362 $150.00
Custom Retroviral Vector using ORF Library with HA Tag Any human, mouse, or rat gene from our extensive ORF library, available in a retroviral vector with a C-term HA tag.
Corresponding ORF vector must be ordered with this service. Maximum insert size that can be packaged is 4.5 kb.
1.0 μg C363 $300.00
Custom Retroviral Vector using ORF Library with GFP Reporter Any human, mouse, or rat gene from our extensive ORF library, available in a retroviral vector with a GFP reporter.
Corresponding ORF vector must be ordered with this service. Maximum insert size that can be packaged is 3.2 kb.
1.0 μg C364 $300.00
Custom Retroviral Vector Custom retrovirus plasmid subcloning. Provide your DNA and we’ll subclone it into a retroviral vector. 1 Service C057 $550.00
Gene Synthesis For any sequence up to 4.5 kb in size. Genes are fully-sequenced. Must be ordered with Custom Retroviral Vector (C362, C363 or C364).
* Minimum charge of $99.00
1.0 μg C098 $0.18/bp USD
Additional cloning services are available.
Custom Retroviruses
Service NameQuant. DNA ProvidedCAT. NO.QuantPRICE
Custom Retrovirus Enough DNA Provided ≥50 μg C058 10 ml of 105 IU/ml $400.00
≥50 μg C059 10 ml of 106 IU/ml $450.00
≥350 μg C060 2x200 μl of 107 IU/ml $700.00
≥350 μg C061 2x200 μl of 108 IU/ml $950.00
DNA Amplification Required <50 μg C064 10 ml of 105 IU/ml $600.00
<50 μg C065 10 ml of 106 IU/ml $650.00
<350 μg C066 2x200 μl of 107 IU/ml $900.00
<350 μg C067 2x200 μl of 108 IU/ml $1150.00
Please Note: For customer-supplied vectors, the customer must indicate prior to the service if any reporter expression will be expected or not. The expected reporter expression, e.g. GFP, should not require induction of expression. Due to differences in excitation/emission wavelengths for different fluorophores, we may not be able to provide infection images for fluorescent reporter expression other than standard GFP/RFP/mCherry.
Titer Upgrades for Existing Retroviruses
Service NameUnitCAT. NO.PRICE
Upgrade from 106 IU/ml Retrovirus to 107 IU/ml Retrovirus 2x200 μl of 107 IU/ml C365 $250.00
Upgrade from 106 IU/ml Retrovirus to 107 IU/ml Retrovirus 2x200 μl of 107 IU/ml C366 $500.00
Retrovirus Controls
ProductUnitCAT. NO.PRICE
pRetroG-CMV-blank Vector 1.0 μg RV001 $135.00
pRetroG-CMV-GFP-HA Vector 1.0 μg RV002 $135.00
pRetroG-CMV-GFP-stop Vector 1.0 μg RV003 $135.00
pRetroG-CMV-GFP-IRES-GFP Vector 1.0 μg RV004 $135.00
pRetroG-CMV-RFP-stop Vector 1.0 μg RV005 $135.00
Retro-CMV Blank Retrovirus 1 ml of 106 IU/ml RVP001 $195.00
Retro-CMV-GFP-HA Retrovirus 1 ml of 106 IU/ml RVP002 $195.00
Retro-CMV-GFP Retrovirus 1 ml of 106 IU/ml RVP003 $195.00
Retro-CMV-GFP-IRES-GFP Retrovirus 1 ml of 106 IU/ml RVP004 $195.00
Retro-CMV-RFP Retrovirus 1 ml of 106 IU/ml RVP005 $195.00
Retrovirus Vectors (click for details):



Additional Info

Workflow

Workflow

FAQs

How long does it take to produce virus?
This will be dependent on a number of factors, including the plasmid you would like to package. Please send all inquiries to [email protected] for a prompt response on the estimated lead time.
What the concentration of transfer vector should I submit?
The volume of DNA needed to produce recombinant virus has been listed alongside each titre available. (see above)
How do you determine titer?
Via RT-PCR
What is the packaging capacity for Retrovirus?
The maximum insert size is <8 kb between the 5'LTR to 3'LTR.
What is the minimum amount of DNA needed for subcloning into a retroviral vector (C057)?
The minimum amount of DNA we require is 10μg for subcloning into a retroviral vector.

Citations

01 Thomas, A. M. et al. "Polysaccharide-modified scaffolds for controlled lentivirus delivery in vitro and after spinal cord injury." J Control Release. 3:421-429 (2013). DOI: 10.1016/j.jconrel.2013.06.013 PubMed: 23791981