What assay do you use to confirm the functionality of the produced antibody?
For mouse monoclonal antibody service, we use Western blotting to ensure the produced antibody have affinity and specificity towards the antigen. Immunohistochemistry and Immunoprecipition tests are also available, however are NOT included in the production service.
What is the shortest length peptide that you can produce an antibody against?
To produce an antibody that has specificity and affinity at acceptable levels, we require a minimum of 20 amino acids.
Can you produce an antibody against a native protein?
Yes, however to guarantee the antibody is effective against the native protein purified native protein must be used for immunization. Recombinant protein or peptide extracts can also be used to immunize, but we can not guarantee these will be effective against the native protein due to folding and other conformational issues. We only guarantee that the immunized protein (native or recombinant) or peptide will be recognized by the produced antibody.
What is the isotype of antibody your production produces?
Mainly our process results in IgG2a and IgG2b antibodies.
What concentration and amount of antigen is required for production if I am supplying the protein/peptide?
For peptide antigens we require at least 2mg, while if it is a protein 5mg with concentrations of 0.5-1.0mg/ml are acceptable.
Do you deliver the Hybridoma cells along with the custom antibody?
Yes, the customer will receive the custom hybridoma cells along with the custom antibody.
What information do I need to provide to obtain a quote?
1) The peptide antigen design
2) Mouse monoclonal or Polyclonal Antibody Production?
3) Are you looking for the standard service or complete service?
Please email this information to email@example.com
We work closely with you to design the process and provide regular updates on the project. You have the option to start or stop the project from any stages of the rabbit monoclonal antibody generation. Customers will be responsible for the material and labour cost incur if chose to stop the project from any stages of the development.