DNA and RNA Ligases
abm offers variety of ligases that enable the preparation of RNA and DNA for subsequent application(s) in next generation whole genome sequencing and library construction.
- Sku: E007
Unit: 1000 U (100 μl)E.coli DNA Ligase is an NAD -dependent enzyme that catalyzes the formation of a phosphodiester bond between cohesive 3′-hydroxyl and 5′-phosphoryl termini of double-stranded DNA (dsDNA). This enzyme is also active on nicked DNA but is not...
- Sku: G467
Unit: 200 U (200 μl)T4 DNA Ligase catalyzes the formation of a phosphodiester bond between the proximal 3’- hydroxyl and 5’- phosphate ends of adjacent, nicked nucleotides of dsDNA, dsRNA or DNA-RNA hybrids. Known to proceed via an adenylated intermediate, this...
- Sku: E010
Unit: 100 μlT4 RNA Ligase 1 catalyzes the ATP-dependent formation of a 3'→5' phosphodiester bond on a broad range of substrates including single-stranded RNA and DNA, oligoribonucleotides, oligodeoxynucleotides, as well as numerous nucleotide derivatives. The...
- Sku: E011
Unit: 100 μlT4 RNA Ligase 2 (dsRNA Ligase) catalyzes the ATP-dependent formation of a 3'→5' phosphodiester bond catalyzing intramolecular and intermolecular RNA strand joining. Unlike T4 RNA Ligase 1 (Cat. No. E010) which ligates single-stranded RNA, T4 RNA...
- Sku: E012
Unit: 100 μlT4 RNA Ligase 2 (Truncated) catalyzes the formation of a phosphodiester bond between a pre-adenylated 5’ phosphate (DNA or RNA) and the 3’ hydroxyl of RNA. This truncated enzyme contains the first 249 amino acids and does not require ATP for...
- Sku: E090
Unit: 10000 U (250 µl)Taq DNA Ligase is a thermostable ligase that catalyzes the formation of a phosphodiester bond between adjacent 5’-phosphate and 3’-hydroxyl groups of duplex DNA. This enzyme is a NAD dependent DNA ligase.