DNA and RNA Ligases

abm offers variety of ligases that enable the preparation of RNA and DNA for subsequent application(s) in next generation whole genome sequencing and library construction.

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  1. Price: $60.00
    T4 DNA Ligase catalyzes the formation of a phosphodiester bond between the proximal 3’- hydroxyl and 5’- phosphate ends of adjacent, nicked nucleotides of dsDNA, dsRNA or DNA-RNA hybrids. Known to proceed via an adenylated intermediate, this...


  2. Price: $125.00~$125.00
    Taq DNA Ligase is a thermostable ligase that catalyzes the formation of a phosphodiester bond between adjacent 5’-phosphate and 3’-hydroxyl groups of duplex DNA. This enzyme is a NAD dependent DNA ligase.


  3. Price: $125.00~$125.00
    T4 RNA Ligase 2 (Truncated) catalyzes the formation of a phosphodiester bond between a pre-adenylated 5’ phosphate (DNA or RNA) and the 3’ hydroxyl of RNA. This truncated enzyme contains the first 249 amino acids and does not require ATP for...


  4. Price: $60.00~$60.00
    T4 RNA Ligase 2 (dsRNA Ligase) catalyzes the ATP-dependent formation of a 3'→5' phosphodiester bond catalyzing intramolecular and intermolecular RNA strand joining. Unlike T4 RNA Ligase 1 (Cat. No. E010) which ligates single-stranded RNA, T4 RNA...


  5. Price: $25.00~$25.00
    T4 RNA Ligase 1 catalyzes the ATP-dependent formation of a 3'→5' phosphodiester bond on a broad range of substrates including single-stranded RNA and DNA, oligoribonucleotides, oligodeoxynucleotides, as well as numerous nucleotide derivatives. The...


  6. Price: $75.00~$75.00
    E.coli DNA Ligase is an NAD -dependent enzyme that catalyzes the formation of a phosphodiester bond between cohesive 3′-hydroxyl and 5′-phosphoryl termini of double-stranded DNA (dsDNA). This enzyme is also active on nicked DNA but is not...

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