FAM115C Protein Vector

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FAM115C Protein Vector (Human) (pPM-N-D-C-His)
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Cat.No.: PV351281
Quantity: 500 ng
Stock: 1-3 Weeks
Price: $950.00
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Data Sheet
Print Version
Gene Insert FAM115C
Description The FAM115C protein expression will be driven by a CMV promoter, with a D Tag at the N terminal and His Tag at the C terminal. Inserts are flanked by and can be excised using NheI and XhoI as long as inserts do not contain any internal NheI or XhoI sites.
Accession Number BC113530
Vector pPM-N-D-C-His
ExpressionMammalian
Promoter CMV Promoter
Vector Size 4797bp
Insert Size 2538bp
Species Human
Tags D/His
Selection Marker Bacterial: Kanamycin. Mammalian: Neomycin.
Appearance Clear Liquid
Storage Buffer 10mM Tris-HCI, 1mM EDTA, pH8.0
Stability 1 year when stored at -20°C or lower in a non-frost free freezer.
Disclaimer 1. Disclaimer for transcript variants: The provided accession number refers to the transcript (mRNA) sequence for this product. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. All sales are final.
2. The provided accession number refers to the transcript (mRNA) sequence for this product. Please verify that this is the desired transcript sequence by cross-referencing. This is important because a single gene can have multiple different transcripts owing to naturally occurring variations. All sales are final.
3. Disclaimer for 'E. coli' protein vectors: The choice of utilizing abm's Escherichia coli based protein vectors for eukaryotic protein production is solely up to the discretion of the customer(s) with respect to expected outcomes for gene-expression, protein folding and post translational modifications.
4. Disclaimer for intended use: All of abm's protein vectors are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its protein vector(s) in therapeutic/diagnostic application(s).
5. Disclaimer for extra amino acids: Cloning may lead to the insertion of some amino acids at either and/or the N and C-terminus of the recombinant protein product which, in most cases, is innocuous to the stability/functionality of the recombinant protein.
6. Disclaimer for the stop codon: The stop codon for the inserted gene is located after the 3' cloning site in our protein vectors. Our protein vectors contain open ORF inserts (no stop codon) to allow for subsequent insertion into other vectors with C-terminal tags or C-terminal fusion proteins if desired. Please note that a stop codon will need to be added by PCR to the end of the gene insert if no tag or fusion protein is needed in subsequent vectors.
Guarantee abm guarantees that the correct ORF construct is provided and the mRNA expression is displayed upon successful transduction. If this is not the case, we will provide a one-time replacement. Customers must provide adequate data to show >80% transfection efficiency with a positive control, plus additional qPCR data to evaluate the level of gene expression. The replacement will not be covered by the same guarantee.

Please note that due to the large number of variables applicable, any further expression analysis (e.g. protein expression) is not covered by the guarantee, as such analysis is dependent on the end user's experimental conditions.
Product Documents
Product References
FAQs
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Are your pPB protein expression vectors high or low copy number plasmids?
Our protein expression vectors are medium copy number plasmids and can be amplified using any standard miniprep or midi/maxi prep kits.

There is no standard protocol that fits all proteins, therefore recombinant protein expression will need to be optimized and determined experimentally.
When the protein is expressed from this vector, which enzyme do I need to use to remove my tag?
The enzyme required to remove the tag (if possible) will depend upon the vector backbone corresponding to your product:

1) pPB-C-His: the His tag is NOT cleavable.

2) pPB-His-GST: the His-GST tag can be cleaved using TEV protease.

3) pPB-His-MBP: the His-MBP tag can be cleaved using TEV protease.

4) pPB-N-His: the His tag can be cleaved with Thrombin.

5) pPM-C-HA: the HA tag is NOT cleavable.

6) pPM-C-His: the His tag is NOT cleavable.

7) pPM-N-D-C-HA: the N-terminal D-tag can be cleaved using TEV protease. The C-terminal HA tag is NOT cleavable.

8) pPM-N-D-C-His: the N-terminal D-tag can be cleaved using TEV protease. The C-terminal His tag is NOT cleavable.

Please see the following page for further details:

https://www.abmgood.com/Protein-Vector.html