GeneCraft-R Express CRISPR sgRNA Synthesis and Screening Kit (S. pyogenes)

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GeneCraft-R Express CRISPR sgRNA Synthesis and Screening Kit (S. pyogenes)
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Cat.No.: G954
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Price: $395.00
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Description abm’s GeneCraft-R Express CRISPR sgRNA Synthesis and Screening Kit (S. pyogenes) is the most comprehensive tool for sgRNA generation and subsequent editing efficiency analysis.

The 2X Express sgRNA Synthesis SuperMix contains all the components required for the in vitro sgRNA transcription(except the sgRNA specific template). High quality sgRNA can be obtained in as little as 30 minutes.

Freshly synthesized sgRNA can be used directly in downstream in vitro screening without any purification. The target template for screening will be PCR-generated using the 2X PCR Bestaq™ MasterMix included in this kit. An optional DNase I treatment followed by spin column purification can be performed on the remaining successfully selected sgRNA(s) to remove DNA template, and leftover NTPs and dNTPs. This optional step could result in a more suitable sgRNA for downstream in vivo studies and applications.
Application
  • Synthesis of sgRNA
  • Screening of sgRNA
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Protocol Click To Open
Storage Store all components at -20°C.
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