Glutathione Agarose Beads

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Product Name Size Cat. No. Price
Glutathione Agarose Resins 10 ml G241 $210.00
Glutathione Agarose Resins 25 ml G289 $395.00
*Pricing in USD
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Detailed Information
Description
Affinity chromatography is a method of separating biochemical mixtures based on highly specific biological interactions. The Glutathione Agarose is developed for purification of fusion-proteins expressing a Glutathione S-transferase (GST) moiety. This GST-glutathione interaction makes one-step purification possible for proteins under native conditions. GST has a high affinity for its substrate, glutathione, which has been covalently bound to a solid support. Impurities are removed and the bound GST-fusion proteins can be eluted by using buffers containing reduced glutathione.
Applications
  • Purification of Glutathione-S-transferase (GST) and GST-tagged fusion proteins.
  • Antibody purification.
  • Assays involving protein-protein and protein-DNA interactions.
  • Structural and functional investigation of proteins.
  • Special Features
  • One-step easy purification protocol from crude lysate.
  • Purification under native conditions.
  • Can be used for proteins with low expression.
  • Can be used for successive cycles.
  • Ready-to-use for any scale of purification.
  • Protein binding capacity: approx. > 8mg recombinant GST/ml gel.
  • Resins and cartridges formats suitable for MPLC, FPLC, AKTA design, batch, gravity, peristaltic pump and syringe.
  • Beads are supplied as 75% (V/V) aqueous suspension (in 20% ethanol).
  • Related Products
    Purified protein fractions can be detected using Anti-GST Tag Antibody
    Data Sheet
    Product Documents
    Product References
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