Human Primary Hepatic Non-Parenchymal Cells

Human Primary Hepatic Non-Parenchymal Cells

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Cat.No.: T4061
Quantity: 1x106 cells/1.0 ml
Price: $625.00
Stock: Out of Stock
Phone: 604-247-2416 (Local), 1-866-757-2414 (Toll free)
Fax: 604-247-2414
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Data Sheet
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Procedure Overview
BioSafety Level II
Organism Homo sapiens
Source Organ Liver
Growth Properties Adherent
Morphology Elongated
Applications For Research Use Only
Propagation Use of PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells in ECM-coated culture vessels with the following conditions. The base medium for this cell line is Prigrow X medium available in ABM, Cat. No. TM100. This cell line needs to be cultured in collagen coated flasks. Atmosphere: air, 95%; Carbon dioxide (CO2), 5%. These cells do not proliferate in vitro .
Disclaimer 1. The CoA for this product (provided upon request) verifies the cell-type specific gene expression via RT-PCR only. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers. All sales are final.
2. We strongly recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for functional freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping).
3. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information.
4. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate.
Product Documents
Product References
Submit a new question:
What is the supply procedure?
The frozen vial of cells will be shipped in Dry ice while T25 flasks by themselves are shipped at room temperature.
Does ''Normal" mean undiseased tissue/organ?
How often do I need to change the media?
The media should be renewed every 2-3 days
How should I store frozen vials of primary cells? Can a frozen vial be put back into liquid nitrogen after delivery?
In most cases, a vial of primary cells shipped in dry ice (-70°C) can be placed back into liquid nitrogen and recovered at a later date by rapid thawing. However it is important to note that the viability of some sensitive cell types may be reduced by the temperature shift of such treatment, making recovery more difficult.

For this reason, we recommend that cells be thawed and placed into culture as soon after receipt as possible. It is best to minimize storage time at -70°C; as this is only used for shipping the cells.

ABM does not warrant the viability of cells stored at -70C after shipments have been received.
How much Pen/Strep should I add to the culture media? (Only where stated in the culturing protocol provided).
We suggest 1% P/S (G255). It should not be required if your lab does not use P/S routinely however.
G255 Penicillin-Streptomycin contains 10,000 units of penicillin (base), 10,000µg of streptomycin (base) per mL in WFI water. It is supplied as a 0.22 µm-filtered, 100X frozen liquid.
What is the recommended storage temperature?
In general, if you received:

Live cells: acclimatize for 3-4 hrs at 37C, 5% CO2 and then change media afterwards.
Frozen cells: Immediately place cells in liquid nitrogen or dry ice; -180C.
Is it possible to freeze the cells again after thawing?
Yes, primary cell lines may be re-freezed after thawing. However, primary cells are usually not good for multiple passaging and they usually last only for up to 10-12 population doublings. Our primary cells are usually provided at passage 2, therefore they can be passaged for an additional 1-2 passages. For long term use, immortalized cells are preferred.
Are these cells plateable?
These are plateable and viable but do not proliferate in culture.