IHC Optimization Service
If you have a newly developed antibody that has not been tested for IHC staining yet, abm will work with you to provide the best IHC staining conditions for your antibody.
Before and After IHC Optimization
For any antibody used for immunohistochemistry staining, optimization of the pretreatment and working concentration is crucial to obtain the best staining results. After fixation of the tissue, proteins are cross linked to one another which prevent some epitopes from binding to the complementary antibody. To improve binding and subsequent staining of the marker of interest, different pretreatment methods are utilized to break the cross linkage of proteins. Antibody concentration will also greatly affect antibody-antigen binding and staining intensity. To obtain the best staining with minimum amount of background, the working concentration for each antibody must be determined experimentally.
"We are happy about the CD4 and CD8 IHC staining your lab did. The quality of the IHC staining is very good."Fugang Zhu, Idera Pharnacueticals Inc
|Service Name||Description||CAT. NO.||PRICE|
|IHC Optimization Service||Optimization of a specific antibody to obtain the best staining results with minimal background. To obtain the best results, 3 methods of pretreatment, and 4 different dilutions will be tested for each case.
Customers to provide:
(1) minimum of 100 µg of the primary antibody, and , (2) one positive control tissue block for the marker of interest
Turnaround time: 2-3 weeks.
Deliverables: Images of all 12 slides will be given to the customer. For delivery of actual slides, please inquire.
|Add‑On Services*||CAT. NO.||PRICE|
|Cell Pellet Processing and Embedding#||HC009||$8.00/block|
|Serial Sectioning||HC007||Inquire - starting at $6.00/slide|
|Step Sectioning||HC008||Inquire - starting at $6.00/slide|
Additional InfoSample Submission Guidelines and Shipping Instructions
Please fill out our Histology Service Request Form, and email it to [email protected] to initiate an inquiry. Before shipping, please fill out our Histology Services Sample Submission Formwith your order number and follow the shipping instructions for your samples.
|Specimen type||Shipping instructions|
|Unstained slides||Place slides in microscope slide boxes. Make sure to seal and wrap the box well.|
|Formalin-Fixed wet tissues||Transfer formalin-fixed sample to 70% alcohol. Seal and wrap the container well to prevent leaks.|
|Fixed cell pellet||Transfer the samples to 70% alcohol. Seal and wrap the container well to prevent leaks.|
|FFPE tissue blocks||Ship tissue blocks in a cassette case or bag. During the month of May to August, ship blocks with gel ice pack to prevent the block from melting due to higher temperatures.|