IHC Optimization Service

If you have a newly developed antibody that has not been tested for IHC staining yet, abm will work with you to provide the best IHC staining conditions for your antibody.


Before and After IHC Optimization
For any antibody used for immunohistochemistry staining, optimization of the pretreatment and working concentration is crucial to obtain the best staining results. After fixation of the tissue, proteins are cross linked to one another which prevent some epitopes from binding to the complementary antibody. To improve binding and subsequent staining of the marker of interest, different pretreatment methods are utilized to break the cross linkage of proteins. Antibody concentration will also greatly affect antibody-antigen binding and staining intensity. To obtain the best staining with minimum amount of background, the working concentration for each antibody must be determined experimentally.




"We are happy about the CD4 and CD8 IHC staining your lab did. The quality of the IHC staining is very good."

Fugang Zhu, Idera Pharnacueticals Inc

Additional Resources:

Service Details

Core Services
Service NameDescriptionCAT. NO.PRICE
IHC Optimization Service Optimization of a specific antibody to obtain the best staining results with minimal background. To obtain the best results, 3 methods of pretreatment, and 4 different dilutions will be tested for each case.

Customers to provide:
(1) minimum of 100 µg of the primary antibody, and , (2) one positive control tissue block for the marker of interest
Turnaround time: 2-3 weeks.

Deliverables: Images of all 12 slides will be given to the customer. For delivery of actual slides, please inquire.
HC015 $350.00/case
Add-On Services*
Have the tissue, but can’t embed it or cut it? Don’t worry, send us the tissue sample/block, and we can do the embedding and/or cutting for you!
Add‑On Services*CAT. NO.PRICE
HC004 $5.00/tissue
Processing of Formalin-Fixed wet tissue suitable for paraffin embedding.

Deliverables: Processed wet tissue suitable for paraffin embedding.
HC005 $4.00/tissue
Embedding processed tissue into FFPE block suitable for sectioning and subsequent staining.

Deliverables: FFPE tissue block
HC009 $8.00/block
Processing of wet cell pellet into FFPE block suitable for sectioning and subsequent staining.

Deliverables: Paraffin embedded cell pellet block
HC006 $4.00/Slide
Sectioning of FFPE blocks to produce unstained slides. Unstained slides can be used for H&E, IHC or dye based staining. All slides are cut at 4 μM unless otherwise specified. Please inquire for Tissue Microarray sectioning.

Deliverables: Unstained FFPE tissue section slides.
HC007 Inquire - starting at $6.00/slide
Multiple sections from the same tissue block cut at set distances from one another by the Microtome

Deliverables: Multiple slides cut in sequence by the Microtome from the same specimen.
HC008 Inquire - starting at $6.00/slide
Multiple sections from the same tissue block cut at set distances from one another by the Microtome

Deliverables: Multiple slides within a sample at set distance from one another
HC016 Inquire
HC017 Inquire
HC018 Inquire
Digital scans of stained slides at 10X, 20X or 40X magnification. This service is offered in conjunction with IHC, H&E or special staining services.

Deliverables: Digital scan will be digitally provided to the customer.
HC011 Inquire
Image analysis of stained slides using professional software.

Deliverables: Analysis results will be sent digitally to the client.
#Minimum order of 10 tissue samples is required.

Additional Info

Sample Submission Guidelines and Shipping Instructions
Please fill out our Histology Service Request Form, and email it to [email protected] to initiate an inquiry. Before shipping, please fill out our Histology Services Sample Submission Formwith your order number and follow the shipping instructions for your samples.
Specimen typeShipping instructions
Unstained slides Place slides in microscope slide boxes. Make sure to seal and wrap the box well.
Formalin-Fixed wet tissues Transfer formalin-fixed sample to 70% alcohol. Seal and wrap the container well to prevent leaks.
Fixed cell pellet Transfer the samples to 70% alcohol. Seal and wrap the container well to prevent leaks.
FFPE tissue blocks Ship tissue blocks in a cassette case or bag. During the month of May to August, ship blocks with gel ice pack to prevent the block from melting due to higher temperatures.

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