Klenow Fragment (3’→5’ Exo-)

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Klenow Fragment (3’→5’ Exo-)
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Cat.No.: E038
Quantity: 500 U (100 μl)
Price: $45.00
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Data Sheet
Print Version
Description Klenow Fragment (3’→5’ Exo-) is the large fragment of E. coli DNA Polymerase I which retains polymerase activity but lacks 5’→3’ exonuclease activity and has mutations that effectively abolish the inherent 3’→5’ exonuclease activity.
Application
  • Dideoxy DNA sequencing of single- or double-stranded DNA templates
  • cDNA second-strand synthesis
  • Generate single-stranded DNA probes using random primers
  • Site-directed DNA mutagenesis using synthetic oligonucleotides
  • Form Enzyme supplied with 10X Reaction Buffer
    Image
    Concentration 5 U/μl
    Storage Store all components at -20°C.
    Storage Buffer 25 mM Tris-HCl (pH 7.5), 0.1 mM EDTA, 1 mM DTT, and 50% (v/v) Glycerol.
    Notes One unit is defined as the amount of Klenow Fragment (3’→5’ Exo-) that catalyzes the incorporation of 10 nmol of dNTP into acid insoluble material in 30 minutes at 37°C using poly(dA-dT):poly(dA-dT) as a template:primer.

    This product is distributed for laboratory research only.
    Caution: Not for diagnostic use.
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