Lentiviral Blank Control Vectors
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Selection Marker Qty Cat. No. Price
pLenti-III-Blank Vector Bacterial: Kanamycin
Mammalian: Puromycin
1.0 µg LV587 $135.00
pLenti-EF1a-Blank Vector Bacterial: Kanamycin
Mammalian: Puromycin
1.0 µg LV588 $135.00
pLenti-UbC-Blank Vector Bacterial: Kanamycin
Mammalian: Puromycin
1.0 µg LV589 $135.00
pLenti-CMV-GFP-2A-Puro-Blank Vector Bacterial: Kanamycin
Mammalian: Puromycin
1.0 µg LV590 $135.00
pLenti-CMV-RFP-2A-Puro-Blank Vector Bacterial: Kanamycin
Mammalian: Puromycin
1.0 µg LV591 $135.00
pLenti-PGK-Blank Vector Bacterial: Kanamycin
Mammalian: Puromycin
1.0 µg LV627 $135.00
pLenti-PGK-GFP Vector Bacterial: Kanamycin
Mammalian: Puromycin
1.0 µg LV628 $135.00
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Product Details
Description
  Empty versions of our most popular vectors to use as a control during your lentiviral experiments.
Data Sheets
Notes
NOT FOR RESALE without prior written consent of ABM. This product is distributed for laboratory research only. Caution: Not for diagnostic use.
Product Documents
Product References
FAQs
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What is the difference between Retro-, Lenti-, and Adeno- viruses?
Retrovirus: Classic, can integrate into the genome but with low transduction efficiency. They are useful for gene transfer and protein expression in cells that have low transfection efficiency with other transfection reagents.

Lentivirus: Can integrate into the genome with relatively high transduction efficiency and they are very useful for cells that have low transfection efficiency with other transfection reagents. No special competent cells required, as they are stable plasmids. Lentiviruses are a powerful tool for stable gene transfer to both dividing and non-dividing cells in vitro and in vivo.

Adenovirus: Only work transiently (about 7 days) but have almost 100% transduction efficiency. Adenoviruses can infect a broad range of cell types with the highest efficiency and infection is not dependent on active host cell division. A second key feature is that high virus titers and high-level gene expression can be obtained in most mammalian cells.
What are the correct concentration units for each recombinant viral particle?
For lentiviruses and retroviruses, they are measured in CFU/ml (colony-forming units per millilitre). Transduction with lentiviruses and retroviruses can cause the formation of colonies, which can be quantified for concentration. For AAV the titer is measured as genome copies per mL (GC/mL). Adenoviruses are measured as PFU/ml (plaque-forming units per millilitre). Transduction with adenoviruses will kill packaging cells, forming plaques in the process for quantification. The concentration for all three types of viruses can also be classified as IU/ml (Infectious Units/ml). Ultimately, the units refers to the viral particles and different units reflect the different assays involved.
How long after transduction can the infection efficiency be observed?
You can observe transduction efficiency from 48 hours up to 5 days after infection.
What generation is your lentivirus?
It is third generation.

What is our lentivirus based on?
Our system is based on inactivated HIV. All our packaging plasmids cannot be integrated into the host and they are transiently expressed only.
What is the function of 3’ acceptor sites in lentiviral vectors?
It's for biosafety reason. The 5' splice donor and 3' acceptors sites enhance the biosafety of the vector by facilitating removal of the packaging sequence and RRE such that expression of the gene of interest in the transduced host cell is no longer Rev-dependent (Dull et al., 1998). It will inhibit viral production in stable cell lines.
Is your lentiviral system Tat dependent or independent?
Tat-Independent.
How do you ensure that your lentivirus is replication-defective?
Lentiviral vector stocks are tested for the presence of replication-competent lentivirus (RCL) by monitoring p24 antigen expression in the culture medium of transduced 293T cells for 30 days. Since the vectors are replication-defective, no amplification of the p24 expression would normally be expected. However, if there were an increase in P24 signal over time, it would be indicative of RCL contamination. The p24 expression is assayed by qPCR.
How efficient is the lentivirus transduction?
This depends on the cell line or type as each one is different. In general, the lentivirus is very efficient in most cell lines with 100% for 293 and other commonly used cells. The lowest efficiency observed is 10% and customers have to do evaluation for a specific cell line using EGFP lentivirus. For customers that just need to generate a stable cell line, 1% trandsuction efficiency is enough as every transduced cell is permanently integrated with the vector. Therefore, 10ml of 10^6 cfu/ml would contain more than enough transduced cells.
How do I increase viral titre in my target cells?
Usually, there is no need to increase viral titres for in-vitro experiments. In our company, we usually produce the virus in 293T cells and then infect the virus to the target cells. To increase the titre in the viral production, cell density should be around 80%. Packaging mix is also very important for viral production. Sometimes, lipid-based packaging mix does not work very well. You can try calcium phosphate transfection. If that also does not work, you can try our Lentifectin, which works very well in producing recombinant lentivirus. We use Lentifectin for our own custom services.
What is the difference between the promoters?
EF1a promoter offers highest percentage of cells expressing transgene, follow by PGK and UBC. However, PGK promoter offers higher gene expression level in positive cells.
CMV: Immediate-early Cytomegalovirus virus promoter for high-level expression in a wide variety of mammalian cell lines
EF1-1a: Human elongation factor 1a-subunit promoter for high-level expression
UbC: Human ubiquitin C promoter for high-level expression that is equivalent across a broad range of species and tissue types
SV40: Simian virus 40 promoter for high-level expression. Permits replication in cell lines expressing the large T antigen
PGK: Murine Phosphoglycerate Kinase-1 promoter for long-term persistent expression in cells that are susceptible to promoter silencing from methylation or histone deacetylation , such as undifferentiated embryonic stem (ES) cells.
Does your system work with our packaging mix?
This will depend on your system. It may or may not work. Ours has been tested to be compatible with OpenBiosystems and Invitrogen but we have not tested with others suppliers.
How come after infection, the target cells’ life spans are shorter than the negative control (target cells without the lentivirus)?
For the infection process, the supernatant is used to infect the target cells. The supernatant was collected from the viral production stage and based on DMEM media that have been depleted of nutrients at the point of harvest. These characteristics of the supernatant may create a stressful environment for the target cells. DMEM may not be appropriate for the target cells and/or the depleted media is not enough to maintain the target cells. Furthermore, the lentiviral components make the cells more sensitive to cell death.
Does MOI affect antibiotic resistance?
Higher MOI will provide more copies of the antibiotic resistance gene per cell. Cells containing multiple copies of the resistance gene can withstand higher antibiotic concentrations than those at lower MOIs. The concentration of antibiotic should be adjusted to a level that will select for the population of transduced cells you wish to select for, without going below the minimum antibiotic concentration you have established in your killing curve.
For a lentiviral vector with a kanamycin resistance gene, what concentration of kanamycin should be used?
We recommend 50ug/ml.
Is our system compatible with psPAX2 and pDM2.G?
Yes, it is.
What is the vector design for LV011-a Lenti-GFP?
The GFP gene is cloned via the KpnI and XhoI cut sites into the LV009 - Lenti-Easy-His Tag Vector without the His Tag.
Which strain of competent E.coli do you recommend for amplifying this vector?
We recommend amplifying all of our pLenti vectors in DH5α competent cells
Are the vectors Lenti Promoterless GFP (LV060) and Lenti GFP Control vector (LV011a) high copy no. or low copy no. plasmids?
All of our pLenti vectors are high copy no. plasmids
Does Cat. No. LV588 pLenti-EF1a-Blank Vector contain a start and stop codon downstream of the EF1a promoter?
There are no in frame start and stop codons after the EF1a promoter in this vector. This is a blank control vector and it is designed to not express anything from the EF1a promoter.