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MGST3 Lentivirus (Mouse) (CMV)


MGST3 Lentivirus (Mouse) (CMV) (pLenti-GIII-CMV-C-term-HA)

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Cat.No.: LVP436048
Quantity: 2 x 200 µl
Price: $1,045.00
Titer: >107 IU/ml
Stock: Out of Stock
Phone: 604-247-2416 (Local), 1-866-757-2414 (Toll free)
Fax: 604-247-2414
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Please refer to Custom Lentivirus Subcloning Services for available virus titers and pricing.
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Data Sheet
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Description This ready-to-use lentivirus is part of abm’s Lentiviral Expression System and can be used to overexpress your gene of interest in a wide range of host cells. Lentiviruses have the ability to integrate into the host genome and generate a stable cell line expressing your gene of interest.
Procedure Overview
Gene Insert MGST3
Accession Number NM_0255691,2,3,4,5
Vector pLenti-GIII-CMV-C-term-HA
Vector Size 8071bp
Insert Size 462 bp
Species Mouse
Tags HA
Promoter CMV
Selection Marker Puromycin
Format Virus
Appearance Liquid
Storage Buffer Serum-free DMEM with 5% PEG6000
Stability Lentiviruses are shipped with dry ice. For long term storage, it is recommended to store the viruses at -80°C in small aliquots to avoid repeated freeze-thaw cycles.
Disclaimer 1. Disclaimer for transcript variants: The provided accession number refers to the transcript (mRNA) sequence for this product. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. All sales are final.
2. Disclaimer for gene sequence: The provided accession number refers to the transcript (mRNA) sequence for this product. Please verify that this is the desired transcript sequence by cross-referencing. This is important because a single gene can have multiple different transcripts owing to naturally occurring variations. All sales are final.
3. Disclaimer for intended use: All of abm's vectors and viral particles are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its vector(s) in therapeutic/diagnostic application(s).
4. Disclaimer for extra nucleotides: Cloning may lead to the insertion of extra nucleotides at the 5' or 3' end of the gene of interest which, in most cases, is innocuous to the stability/functionality and the expression of that gene.
5. Disclaimer for the stop codon: The stop codon for the inserted gene is located after the 3' cloning site in our GIII vectors. Our GIII vectors contain open ORF inserts (no stop codon) to allow for subsequent insertion into other vectors with C-terminal tags or C-terminal fusion proteins if desired. Please note that a stop codon will need to be added by PCR to the end of the gene insert if no tag or fusion protein is needed in subsequent vectors.
Guarantee abm guarantees that the correct ORF construct is provided and the mRNA expression is displayed upon successful transduction. If this is not the case, we will provide a one-time replacement. Customers must provide adequate data to show >80% transfection efficiency with a positive control, plus additional qPCR data to evaluate the level of gene expression. The replacement will not be covered by the same guarantee.

Please note that due to the large number of variables applicable, any further expression analysis (e.g. protein expression) is not covered by the guarantee, as such analysis is dependent on the end user's experimental conditions.
Product Documents
Product References
Submit a new question:
How do I calculate MOI?
MOI refers to the number of viral particles per cell used in your infection, e.g. an MOI of 5 indicates that there are five infectious units (IU) or transducing units (TU) for every cell in the well. To determine the multiplicity of infection (MOI), calculate the number of viral particles added per well then divide this number by the number of cells you have seeded into the well.

We would also recommend transducing with a range of MOIs as different cell types may require different MOIs for successful transduction.

MOI = Virus titer (IU/ml) x Virus Volume (ml) / Total cell number