Nucleases

abm offers a range of nucleases that enable the preparation of RNA and DNA for subsequent application(s) in next generation whole genome sequencing and library construction.

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  1. Sku: G028
    Price: $85.00
    Unit: 2000 U (1.0 ml)
    Deoxyribonuclease I (DNase I; EC 3.1.21.1) is a non-specific endonuclease that catalyzes the cleavage of phosphodiester bonds in single/double-stranded DNA, chromatin, and RNA-DNA hybrids. DNase I cleaves DNA to release di-and/or oligonucleotide...


  2. Sku: E091
    Price: $95.00
    Unit: 200 U (100 μl)
    Deoxyribonuclease I (DNase I; EC 3.1.21.1) is a non-specific endonuclease that catalyzes the cleavage of phosphodiester bonds in single/double-stranded DNA, chromatin, and RNA-DNA hybrids. DNase I cleaves DNA to release di-and/or oligonucleotide...


  3. Sku: E015
    Price: $15.00
    Unit: 2000 U (100 μl)
    Exonuclease I, E.coli catalyzes the removal of nucleotides from single-stranded DNA in the 3’→5’ direction, releasing deoxyribonucleoside 5'-monophosphates and leaving 5'-terminal dinucleotides intact. Hydrolysis cannot proceed if the...


  4. Sku: E016
    Price: $35.00
    Unit: 10 000 U (100 μl)
    Exonuclease III, E.coli digests duplex DNA in the 3′→5′ direction from nicked DNA, blunt end DNA, 3′-recessed ends, or 3′-overhangs of less than four bases, and yields nucleoside 5′-phosphates. The DNA degradation proceeds at a uniform...


  5. Sku: E042
    Price: $50.00
    Unit: 1000 U (100 μl)
    Nt.BstNBI (Bst Nickase) is a nicking endonuclease from Bacillus stearothermophilus that cleaves one DNA strand of a double-stranded DNA substrate. This site-specific endonuclease cleaves a single strand of DNA four base pairs away and 3’ of its...


  6. Sku: E093
    Price: $65.00
    Unit: 100 U (100 μl)
    RecBCD (Exonuclease V) is a Mg2 and ATP-dependent enzyme possessing exonuclease activity on linear double stranded (ds) DNA. The exonuclease digests DNA from both the 5’ and 3’ ends to produce oligonucleotides. RecBCD (Exonuclease V) does not...


  7. Sku: G117
    Price: $50.00
    Unit: 25 mg
    RNase A is pancreatic bovine endoribonuclease that cleaves single stranded RNA by hydrolysis at cytosine and uracil residues. RNase A is very useful for the removal of RNA from plasmid DNA preparations.


  8. Sku: E018
    Price: $25.00
    Unit: 500 U (100 μl)
    RNase H (Ribonuclease H), E. coli is an endoribonuclease that specifically hydrolyzes the phosphodiester bonds of RNA strands in RNA-DNA hybrids. This enzyme cleaves the 3'-O-P-bond of RNA and generates 3' hydroxyl and 5' phosphate products. RNase...


  9. Sku: E020
    Price: $85.00
    Unit: 100 U (100 μl)
    RNase III, E.coli is an endoribonuclease that specifically digests dsRNA into dsRNA fragments of 12-15 base pairs that have two or three nucleotide 3′-overhangs with 5' phosphate and 3' hydroxyl termini.


  10. Sku: E049
    Price: $220.00
    Unit: 500 U (50 μl)
    RNase R is an E. coli exoribonuclease which exhibits 3'-to-5' exonuclease activity, efficiently digesting nearly all linear RNA species. This enzyme does not digest circular, lariat, or double stranded RNA with short 3’ overhangs (less than seven...

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