abm offers a range of nucleases that enable the preparation of RNA and DNA for subsequent application(s) in next generation whole genome sequencing and library construction.
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- Sku: E091
Unit: 200 U (100 μl)Deoxyribonuclease I (DNase I; EC 184.108.40.206) is a non-specific endonuclease that catalyzes the cleavage of phosphodiester bonds in single/double-stranded DNA, chromatin, and RNA-DNA hybrids. DNase I cleaves DNA to release di-and/or oligonucleotide...
- Sku: G028
Unit: 2000 U (1.0 ml)Deoxyribonuclease I (DNase I; EC 220.127.116.11) is a non-specific endonuclease that catalyzes the cleavage of phosphodiester bonds in single/double-stranded DNA, chromatin, and RNA-DNA hybrids. DNase I cleaves DNA to release di-and/or oligonucleotide...
- Sku: E089
Unit: 1000 U (100 µl)T5 exonuclease catalyzes the degradation of nucleotides either from the 5’ termini or at nicks of linear or circular dsDNA in a 5’ to 3’ direction. This exonuclease also exhibits ssDNA endonuclease activity in the presence of magnesium ions,...
- Sku: E049
Unit: 500 U (50 μl)RNase R is an E. coli exoribonuclease which exhibits 3'-to-5' exonuclease activity, efficiently digesting nearly all linear RNA species. This enzyme does not digest circular, lariat, or double stranded RNA with short 3’ overhangs (less than seven...
- Sku: E042
Unit: 1000 U (100 μl)Nt.BstNBI (Bst Nickase) is a nicking endonuclease from Bacillus stearothermophilus that cleaves one DNA strand of a double-stranded DNA substrate. This site-specific endonuclease cleaves a single strand of DNA four base pairs away and 3’ of its...
- Sku: E028
Unit: 1000 U (100 μl)T7 Endonuclease I recognizes and cleaves mismatched DNA, heteroduplex DNA, cruciform DNA, Holliday structures and/or junctions, as well as nicked double-stranded DNA. This enzyme has a preference for single stranded over double stranded DNA with...
- Sku: E020
Unit: 100 U (100 μl)RNase III, E.coli is an endoribonuclease that specifically digests dsRNA into dsRNA fragments of 12-15 base pairs that have two or three nucleotide 3′-overhangs with 5' phosphate and 3' hydroxyl termini.
- Sku: E018
Unit: 500 U (100 μl)RNase H (Ribonuclease H), E. coli is an endoribonuclease that specifically hydrolyzes the phosphodiester bonds of RNA strands in RNA-DNA hybrids. This enzyme cleaves the 3'-O-P-bond of RNA and generates 3' hydroxyl and 5' phosphate products. RNase...
- Sku: E016
Unit: 10 000 U (100 μl)Exonuclease III, E.coli digests duplex DNA in the 3′→5′ direction from nicked DNA, blunt end DNA, 3′-recessed ends, or 3′-overhangs of less than four bases, and yields nucleoside 5′-phosphates. The DNA degradation proceeds at a uniform...
- Sku: E015
Unit: 2000 U (100 μl)Exonuclease I, E.coli catalyzes the removal of nucleotides from single-stranded DNA in the 3’→5’ direction, releasing deoxyribonucleoside 5'-monophosphates and leaving 5'-terminal dinucleotides intact. Hydrolysis cannot proceed if the...