Precision™ DNA Polymerase (PCR)

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Precision™ DNA Polymerase (PCR)
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Cat.No.: G078
Quantity: 500 U (100 µl)
Stock: In Stock
Price: $65.00
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Email: order@abmGood.com
Phone: 604-247-2416 (Local), 1-866-757-2414 (Toll free)
Fax: 604-247-2414
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Data Sheet
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Description Frustrated with amplification errors? Let us introduce you to abm’s Precision™ DNA Polymerase. Significant time and effort can be saved by using high fidelity polymerases that eliminate the need for downstream error-correction steps. The use of Precision™ DNA Polymerase for rare DNA templates is imperative as small amounts of DNA are especially prone to high mutant frequencies. abm’s Precision™ DNA Polymerase is the ideal choice for applications demanding high fidelity PCR products.

With abm’s Precision™ DNA Polymerase, you can expect
• Outstanding accuracy and efficiency
• Error-free PCR products for downstream processes
Literature Click To Open
Application • Whole genome sequencing
• Site-directed mutagenesis
• Blunt-end cloning
• Specific amplification of difficult templates (i.e. GC-Rich)
• Low copy PCR assays
Form Enzyme supplied with 5X buffer, MgSO4 and 5X GC Enhancer
Picture 1
Protocol Click To Open
Concentration 5U/µl
Storage Store all components at -20 °C.
Notes This product is ISO-13485 certified.
Product Documents
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FAQs
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What is the Mg2+ concentration in the buffer?
Final concentration Mg2+ in reaction is 1.5mM.
How can I optimize the reaction for multiplexing?
The polymerase and Mg amounts very often need to be optimized in multiplexing and this will need to be evaluated per case by the end user. Primer concentrations for each amplicon in multiplexing would also need to be optimized. The general rule would be lowering the primer concentrations of the strongly amplified targets and increasing the primer concentrations of the weakly amplified targets.