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Recombinant Human CCL1
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|Alternative Names||C-C motif chemokine 1, Small-inducible cytokine A1, I-309
Recombinant Human T lymphocyte-Secreted Protein I-309 (CCL1)
|Description||CCL1 cDNA encodes a 96 amino acid residue precursor protein which is cleaved to yield a 73 amino acid residue mature protein that binds to CCR8. A glycoprotein, CCL1 is secreted by activated T cells and is chemotactic for monocytes but not for neutrophils.|
|Molecular Weight||8.8 kDa|
|Endotoxin Level||<1.0 EU/μg of recombinant protein as determined by the LAL method.|
|Purity||>95% as determined by SDS-PAGE|
|Formulation||Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein|
|Reconstitution||Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin|
|Storage||The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.|
|Usage||For research use only. Not for diagnostic or therapeutic use.|
· Endotoxin is generally measured in Endotoxin Units per milliliter (EU/mL).
· For recombinant proteins: EU is reported per microgram of protein.
· One EU = 0.1-0.2 ng endotoxin/µg of protein.
· At abm, we do the LAL chromogenic assays that can detect down to 0.01 EU/ml.