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Recombinant Human CCL21
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|Alternative Names||C-C motif chemokine 21, 6Ckine, Beta-chemokine exodus-2, SLC, Small-inducible cytokine A21, SCYA21
Recombinant Human Secondary Lymphoid-Tissue Chemokine (CCL21)
|Description||CCL21, which is a member of the family of CC-Chemokines, has a unique 36 (murine) or 37 (human) amino acid carboxyl-terminal extension not seen in other chemokines. CCL21 selectively stimulates the chemotaxis of adult T lymphocytes and is expressed preferentially in lymph node tissue. CCL21 is critical for adult T cell adhesion to high endothelial venules in lymph nodes, a rate-limiting step for T cell trafficking through nodal tissue.|
|Molecular Weight||12.0 kDa|
|Endotoxin Level||<1.0 EU/μg of recombinant protein as determined by the LAL method.|
|Purity||>95% as determined by SDS-PAGE|
|Bioactivity||Determined by its ability to chemoattract total lymphocyte population using a concentration range of 10-100 ng/mL.|
|Formulation||Recombinant CCL21 was lyophilized from a 0.2 μm filtered solution in 2.5% glycine, 0.5% sucrose, 0.01% Tween80, 5 mM Glutamic acid, pH 4.5.|
|Reconstitution||A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.|
|Storage||The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.|
|Usage||For research use only. Not for diagnostic or therapeutic use.|
· Endotoxin is generally measured in Endotoxin Units per milliliter (EU/mL).
· For recombinant proteins: EU is reported per microgram of protein.
· One EU = 0.1-0.2 ng endotoxin/µg of protein.
· At abm, we do the LAL chromogenic assays that can detect down to 0.01 EU/ml.