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Recombinant Human EGFL7

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Product Source Size Cat. No. Price
Recombinant Human EGFL7 Insect cells25 µg Z101535 $660.00
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Data Sheet
Print Version
Alternative Names EGF-like protein 7, Multiple epidermal growth factor-like domains protein 7, MEGF7, EGF-L7, NOTCH4-like protein, Vascular endothelial statin, VE-statin, Zneu1
Recombinant Human Epidermal Growth Factor-Like Protein 7 (EGFL7)
Description EGFL7 is an 31 kDa secreted protein that contains an Emilin-like (EMI) domain, and two epidermal growth factor (EGF) domains - one of which binds calcium. Based on these domains, it has been hypothesized that EGFL7 may self-assemble, similar to extracellular matrix (ECM) proteins and, thus, incorporate into ECM. EGFL7 has been reported to stimulate cell adhesion as well as motility in a manner similar to ECM proteins. EGFL7 has been shown to be primarily expressed by developing ECs but also by primordial germ cells and some central nervous system neurons. Interestingly, EGFL7 expression markedly decreases in ECs in postnatal life, but can be strongly up-regulated after various tissue injuries that lead to increased angiogenic responses.
Gene Symbol EGFL7
Gene ID 51162
Accession No. Q9UHF1
Source Insect cells
Appearance Lyophilized Powder
Molecular Weight 31.0 kDa
Endotoxin Level <1.0 EU/μg of recombinant protein as determined by the LAL method
Purity >95% as determined by SDS-PAGE
Bioactivity The activity was determined by the ability to bind human NOTCH1 in functional ELISA.
Formulation Recombinant Human EGFL7 is lyophilized from a 0.2 μm filtered solution (pH 7.0) in 100 mM glycine, 10 mM NaCl, 10% glycerol and 5% trehalose.
Reconstitution A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Storage This protein can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage For research use only. Not for diagnostic or therapeutic use.
Product Documents
Product References
FAQs
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How are endotoxin levels measured?
1. For estimating the endotoxin levels; we use the LAL (Limulus Amebocyte Lysate) method: The lysate from horseshoe crab amebocytes clots in the presence of very low endotoxin. This reaction is the basis of the Limulus amebocyte lysate (LAL) assay which was approved by the FDA in 1970.

· Endotoxin is generally measured in Endotoxin Units per milliliter (EU/mL).

· For recombinant proteins: EU is reported per microgram of protein.

· One EU = 0.1-0.2 ng endotoxin/µg of protein.

· At abm, we do the LAL chromogenic assays that can detect down to 0.01 EU/ml.
With regard to the BSA levels in some Growth Factors and Cytokines, can you please provide an explanation as to why they are so high?
The amount of BSA, as part of the formulation of a protein, can vary considerably depending on how much BSA was deemed optimum/necessary for protein stability in combination with /in-lieu of - other possible additives. The aforementioned formulations are somewhat analogous to the “carrier” versions of many formulations from “R and D systems” that have as high as 50 µg of BSA per µg of the recombinant protein product. If, needed or desired, abm scientists can substitute BSA for other stabilizing additives for most formulations.
Are your Escherichia coli sourced growth factors: 1) Human derived materials free? 2) Recombinant proteins free?
Yes, all of abm's growth factors made in Escherichia coli using recombinant technology contain no human derived-products or other recombinant proteins. In the rare cases of BSA presence, this will be mentioned in the product's formulation.