Search Growth Factors & Cytokines By Gene Symbol
Recombinant Human FGF8
|Cat. No.||Product Name||Qty.||Promo/% Off||Amount|
|*Notes about Promo Codes: For NON-DISCOUNT promo codes (i.e. free items or shipping) please enter them during check out in the Notes/Promo Code field. To enter a DISCOUNT promo code enter it into the corresponding box in the 'Promo/% Off' column and then click 'Update' for the given product. If the code is valid for that product the discount percentage will display in place of the box and the amount will become the discounted total for that product(s). If the code is not valid it will remain in the box, please double check the expiry and spelling, and if you feel the code should still be accepted please contact us.|
|Alternative Names||FGF-8, Androgen-induced growth factor, AIGF, Heparin-binding growth factor 8, HBGF-8
Recombinant Human Fibroblast Growth Factor 8 (FGF8)
|Description||FGF-8 is a heparin binding protein of the FGF family which works to control cell division, differentiation, and function. These proteins are especially prominent during prenatal development and postnatal growth. FGF-8 can be spliced in four different ways. The products of the FGF-8 splicing events are as follows: FGF8A, 8B, 8E and 8F. This recombinant product represents isoform 8B.|
|Molecular Weight||22.5 kDa|
|Endotoxin Level||<1.0 EU/μg of recombinant protein as determined by the LAL method.|
|Purity||>95% as determined by SDS-PAGE|
|Bioactivity||The bioactivity was determined in a NIH/3T3 cell proliferation assay. The ED50 was in the range of 0.05 - 0.5 ng/ml.|
|Formulation||Lyophilized from a 0.2 μm filtered solution in 20mM phosphate buffer, 0.2 mM EDTA and 1M NaCl (pH 8.0)|
|Reconstitution||A quick spin of the vial followed by reconstitution at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.|
|Storage||The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.|
|Usage||For research use only. Not for diagnostic or therapeutic use.|
· Endotoxin is generally measured in Endotoxin Units per milliliter (EU/mL).
· For recombinant proteins: EU is reported per microgram of protein.
· One EU = 0.1-0.2 ng endotoxin/µg of protein.
· At abm, we do the LAL chromogenic assays that can detect down to 0.01 EU/ml.