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Recombinant Human FGF8

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Product Source Size Cat. No. Price
Recombinant Human FGF8 E. coli50 µg Z101705 $70.00
1.0 mg Z101709 $1,200.00
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Data Sheet
Print Version
Alternative Names FGF-8, Androgen-induced growth factor, AIGF, Heparin-binding growth factor 8, HBGF-8
Recombinant Human Fibroblast Growth Factor 8 (FGF8)
Description FGF-8 is a heparin binding protein of the FGF family which works to control cell division, differentiation, and function. These proteins are especially prominent during prenatal development and postnatal growth. FGF-8 can be spliced in four different ways. The products of the FGF-8 splicing events are as follows: FGF8A, 8B, 8E and 8F. This recombinant product represents isoform 8B.
Gene Symbol FGF8
Gene ID 2253
Accession No. P55075
Source E. coli
Appearance Lyophilized Powder
Molecular Weight 22.5 kDa
Endotoxin Level <1.0 EU/μg of recombinant protein as determined by the LAL method.
Purity >95% as determined by SDS-PAGE
Gel Image Click To Enlarge
Bioactivity The bioactivity was determined in a NIH/3T3 cell proliferation assay. The ED50 was in the range of 0.05 - 0.5 ng/ml.
Bioactivity Data Click To Enlarge
Formulation Lyophilized from a 0.2 μm filtered solution in 20mM phosphate buffer, 0.2 mM EDTA and 1M NaCl (pH 8.0)
Reconstitution A quick spin of the vial followed by reconstitution at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Storage The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage For research use only. Not for diagnostic or therapeutic use.
Product Documents
Product References
FAQs
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How are endotoxin levels measured?
1. For estimating the endotoxin levels; we use the LAL (Limulus Amebocyte Lysate) method: The lysate from horseshoe crab amebocytes clots in the presence of very low endotoxin. This reaction is the basis of the Limulus amebocyte lysate (LAL) assay which was approved by the FDA in 1970.

· Endotoxin is generally measured in Endotoxin Units per milliliter (EU/mL).

· For recombinant proteins: EU is reported per microgram of protein.

· One EU = 0.1-0.2 ng endotoxin/µg of protein.

· At abm, we do the LAL chromogenic assays that can detect down to 0.01 EU/ml.
With regard to the BSA levels in some Growth Factors and Cytokines, can you please provide an explanation as to why they are so high?
The amount of BSA, as part of the formulation of a protein, can vary considerably depending on how much BSA was deemed optimum/necessary for protein stability in combination with /in-lieu of - other possible additives. The aforementioned formulations are somewhat analogous to the “carrier” versions of many formulations from “R and D systems” that have as high as 50 µg of BSA per µg of the recombinant protein product. If, needed or desired, abm scientists can substitute BSA for other stabilizing additives for most formulations.
Are your Escherichia coli sourced growth factors: 1) Human derived materials free? 2) Recombinant proteins free?
Yes, all of abm's growth factors made in Escherichia coli using recombinant technology contain no human derived-products or other recombinant proteins. In the rare cases of BSA presence, this will be mentioned in the product's formulation.