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Recombinant Human FLT3 Ligand (FLT3LG)
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|Alternative Names||FLT-3 ligand, FLT3L
Recombinant Human Fms-Related Tyrosine Kinase 3 Ligand (FLT3LG)
|Description||Known to bind to cells that express the tyrosine kinase receptor, FLT3LG promotes long-term expansion and differentiation of human pro-B cells. Human FLT3LG also stimulates the proliferation of cells expressing murine FLT-3 receptors. In combination with SCF and IL3, FLT3LG can cause expansion of cells with the marker spectrum CD34(+)/CD38(-). Alone, FLT3LG supports the survival of precursors in the lineage of blood-forming cells such as CFU-GM, CFU-GEMM, and the very primitive high proliferative potential colony-forming cells, HPP-CFC. FLT3LG has only marginal effects on progenitors for erythroid cells and megakaryocytes. Recombinant Human FLT3LG is a glycosylated, monomeric protein (with intra-chain disulfide bonds) that runs at approximately 26 kDa on a reducing SDS-PAGE.|
|Molecular Weight||17.0 kDa|
|Endotoxin Level||<1.0 EU/μg of recombinant protein as determined by the LAL method|
|Purity||>95% as determined by SDS-PAGE|
|Bioactivity||The ED(50) as determined by the dose-dependent stimulation of the proliferation of Human AML5 cells was found to be in the range of 1.0 ng/mL.|
|Formulation||Lyophilized from a 0.2 μm filtered PBS solution pH 7.0|
|Reconstitution||A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.|
|Storage||The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.|
|Usage||For research use only. Not for diagnostic or therapeutic use.|
· Endotoxin is generally measured in Endotoxin Units per milliliter (EU/mL).
· For recombinant proteins: EU is reported per microgram of protein.
· One EU = 0.1-0.2 ng endotoxin/µg of protein.
· At abm, we do the LAL chromogenic assays that can detect down to 0.01 EU/ml.