Search Growth Factors & Cytokines By Gene Symbol
Recombinant Human Follistatin (FST)
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|Alternative Names||FS, Activin-binding protein|
|Description||Expressed in the pituitary, ovaries, and in some other tissues, FST is a high affinity activin binding protein that can act as an activin antagonist. FST was originally discovered through its suppressive effect of the expression and secretion of the pituitary follicle stimulating hormone. Besides activin, FS has also been shown to bind with multiple BMPs, Myostatin, GDF-11, and TGF-beta-1. Human Follistatin contains three cysteine-rich domains (called Follistatin-like domains), 3 Kazal-like domains and one TGF-beta binding domain.|
|Molecular Weight||31.0 kDa|
|Endotoxin Level||<1.0 EU/μg of recombinant protein as determined by the LAL method.|
|Purity||>95% as determined by SDS-PAGE|
|Bioactivity||The ED(50) was determined by the ability to neutralize Activin A inhibitory effect of mouse MPC-11 cells and was determined to be 0.1-0.4 µg/mL in the presence of 7.5 ng/mL Activin A.|
|Formulation||Recombinant Human Follistatin was lyophilized at 1.0 mg/mL containing no additives.|
|Reconstitution||A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.|
|Storage||The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.|
|Usage||For research use only. Not for diagnostic or therapeutic use.|
· Endotoxin is generally measured in Endotoxin Units per milliliter (EU/mL).
· For recombinant proteins: EU is reported per microgram of protein.
· One EU = 0.1-0.2 ng endotoxin/µg of protein.
· At abm, we do the LAL chromogenic assays that can detect down to 0.01 EU/ml.