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Recombinant Human Fractalkine (CX3CL1)
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|Alternative Names||C-X3-C motif chemokine 1, CX3C membrane-anchored chemokine, Neurotactin, Small-inducible cytokine D1|
|Description||Fractalkine is a type I membrane protein that signals through the CX3CR1 receptor. The extracellular domain of Fractalkine is released by protease cleavage – producing soluble Fractalkine. Fractalkine has been found in many tissues and shown to be up-regulated in endothelial cells and microglia in response to inflammatory signals. Fractalkine is also involved in leukocyte adhesion as well as attracting monocytes, microglia cells and NK cells.|
|Molecular Weight||8.9 kDa|
|Endotoxin Level||<1.0 EU/μg of recombinant protein as determined by the LAL method.|
|Purity||>95% as determined by SDS-PAGE|
|Bioactivity||Measured by its ability to chemoattract BaF3 mouse pro-B cells transfected with mouse CX3CR1. The ED(50) for this effect is typically 20100 ng/mL|
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein|
|Reconstitution||Reconstitute at 25 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin|
|Storage||The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.|
|Usage||For research use only. Not for diagnostic or therapeutic use.|
· Endotoxin is generally measured in Endotoxin Units per milliliter (EU/mL).
· For recombinant proteins: EU is reported per microgram of protein.
· One EU = 0.1-0.2 ng endotoxin/µg of protein.
· At abm, we do the LAL chromogenic assays that can detect down to 0.01 EU/ml.