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Recombinant Human IFNB1
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|Alternative Names||IFN-beta, Immune interferon, Fibroblast interferon, Leukocyte interferon, B cell interferon, Type I interferon
Recombinant Human Interferon-Beta 1A (IFNB1)
|Description||IFN-beta is produced mainly by fibroblasts and some epithelial cell types. The synthesis of IFN-beta can be induced by common inducers of interferons including viruses, double-stranded RNA, and micro-organisms. It is induced also by some cytokines such as TNF and IL-1. IFN-beta binds to the same receptor as IFN-alpha. IFN-beta is involved in the regulation of unspecific humoral immune responses and immune responses against viral infections. IFN-beta increases the expression of HLA class 1 antigens and blocks the expression of HLA class 2 antigens stimulated by IFN-gamma. IFN-beta stimulates the activity of natural killer cells and hence also antibody-dependent cytotoxicity. IFN-beta shows antiproliferative activity against a number of cell lines established from solid tumors.|
|Molecular Weight||19.0 kDa|
|Endotoxin Level||<1.0 EU/μg of recombinant protein as determined by the LAL method.|
|Purity||>95% as determined by SDS-PAGE|
|Bioactivity||The activity was determined by the viral resistance assay of Human WISH cells was found to be in the range of 3.0 x 10^8 IU/mg.|
|Formulation||Recombinant IFN-b 1a was lyophilized from a 0.2 μm filtered sodium acetate solution pH 4.8.|
|Reconstitution||A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.|
|Storage||The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.|
|Usage||For research use only. Not for diagnostic or therapeutic use.|
· Endotoxin is generally measured in Endotoxin Units per milliliter (EU/mL).
· For recombinant proteins: EU is reported per microgram of protein.
· One EU = 0.1-0.2 ng endotoxin/µg of protein.
· At abm, we do the LAL chromogenic assays that can detect down to 0.01 EU/ml.