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Recombinant Human IGF1

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Product Source Size Cat. No. Price
Recombinant Human IGF1 E. coli100 µg Z100385 $60.00
1.0 mg Z100389 $100.00
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Data Sheet
Print Version
Alternative Names IGF-I, Mechano growth factor, MGF, Somatomedin-C, IBP1
Recombinant Human Insulin Like Growth Factor-I (IGF1)
Description The Insulin Growth Factors belong to the Insulin gene family, the prototypic members of which include insulin and relaxin. Also known as somatomedin C, IGF1 is known to stimulate the proliferation of a wide range of cells including muscle, bone, and those of the cartilage tissue. A dominant effector of the growth hormone, mature IGF1 is generated by proteolytic removal of the N- and C- terminal pro-peptide regions. Mature human IGF1 shares 94% and 96% amino acid sequence identity with mouse and rat IGF1, respectively, while exhibiting cross-species activity. Circulating IGF1 is produced by hepatocytes while local IGF1 is produced by various other tissues in which it exerts paracrine effects. Recombinant Human IGF1 is a globular, 7.7 kDa protein that contains 3 native intra-molecular disulfide bonds.
Gene Symbol IGF1
Gene ID 3479
Accession No. P01343
Source E. coli
Appearance Lyophilized Powder
Molecular Weight 7.7 kDa
Endotoxin Level <1.0 EU/μg of recombinant protein as determined by the LAL method
Purity >95% as determined by SDS-PAGE
Gel Image Click To Enlarge
Bioactivity The ED50 as determined by a cell proliferation assay using MCF-7 cells was found to be ≤20 ng/ml
Formulation Lyophilized from a 0.2 μm filtered solution in sodium phosphate (pH 6.5) and 150mM NaCl
Reconstitution A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Storage The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage For research use only. Not for diagnostic or therapeutic use.
Product Documents
Product References
FAQs
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How are endotoxin levels measured?
1. For estimating the endotoxin levels; we use the LAL (Limulus Amebocyte Lysate) method: The lysate from horseshoe crab amebocytes clots in the presence of very low endotoxin. This reaction is the basis of the Limulus amebocyte lysate (LAL) assay which was approved by the FDA in 1970.

· Endotoxin is generally measured in Endotoxin Units per milliliter (EU/mL).

· For recombinant proteins: EU is reported per microgram of protein.

· One EU = 0.1-0.2 ng endotoxin/µg of protein.

· At abm, we do the LAL chromogenic assays that can detect down to 0.01 EU/ml.
With regard to the BSA levels in some Growth Factors and Cytokines, can you please provide an explanation as to why they are so high?
The amount of BSA, as part of the formulation of a protein, can vary considerably depending on how much BSA was deemed optimum/necessary for protein stability in combination with /in-lieu of - other possible additives. The aforementioned formulations are somewhat analogous to the “carrier” versions of many formulations from “R and D systems” that have as high as 50 µg of BSA per µg of the recombinant protein product. If, needed or desired, abm scientists can substitute BSA for other stabilizing additives for most formulations.
Are your Escherichia coli sourced growth factors: 1) Human derived materials free? 2) Recombinant proteins free?
Yes, all of abm's growth factors made in Escherichia coli using recombinant technology contain no human derived-products or other recombinant proteins. In the rare cases of BSA presence, this will be mentioned in the product's formulation.