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Recombinant Human IGF2

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Product Source Size Cat. No. Price
Recombinant Human IGF2 E. coli10 µg Z102345 $40.00
100 µg Z102347 $140.00
1.0 mg Z102349 $950.00
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Data Sheet
Print Version
Alternative Names Insulin-like growth factor II, multiplication stimulating activity, MSA, Somatomedin-A, T3M-11-derived growth factor, PP1446
Description Insulin-like growth factor 2 (IGF2) is one of the mitogenic, polypeptide growth factors that belongs to the insulin family, due to its structural and functional similarity to insulin. IGF2 is mainly produced in the liver and several other tissues at certain times. IGF2 expression can be found in many tissues usually with autocrine, paracrine and endocrine functions. IGF2 mainly acts as a growth promoting hormone during gestation, and does so by binding to the tyrosine kinase type I receptor (IGF1R) as well as the IGF2 receptor cation-independent mannose 6-phosphate receptor. Mature IGF2 is highly conserved between human, bovine, and porcine species. Recombinant Human IGF2 is a single, non-glycosylated, polypeptide chain of 7.8 kDa.
Gene Symbol IGF2
Gene ID 3481
Accession No. P01344
Source E. coli
Appearance Lyophilized Powder
Molecular Weight 7.8 kDa
Endotoxin Level <1.0 EU/μg of recombinant protein as determined by the LAL method
Purity >95% as determined by SDS-PAGE
Gel Image Click To Enlarge
Bioactivity The ED50 as determined by dose dependent proliferation of MCF7 cells was <1ng/ml
Bioactivity Data Click To Enlarge
Formulation Lyophilized from a 0.2 µm filtered solution in Tris and NaCl
Reconstitution Reconstitute in sterile PBS at 100 µg/mL
Storage The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage For research use only. Not for diagnostic or therapeutic use.
Product Documents
Product References
FAQs
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How are endotoxin levels measured?
1. For estimating the endotoxin levels; we use the LAL (Limulus Amebocyte Lysate) method: The lysate from horseshoe crab amebocytes clots in the presence of very low endotoxin. This reaction is the basis of the Limulus amebocyte lysate (LAL) assay which was approved by the FDA in 1970.

· Endotoxin is generally measured in Endotoxin Units per milliliter (EU/mL).

· For recombinant proteins: EU is reported per microgram of protein.

· One EU = 0.1-0.2 ng endotoxin/µg of protein.

· At abm, we do the LAL chromogenic assays that can detect down to 0.01 EU/ml.
With regard to the BSA levels in some Growth Factors and Cytokines, can you please provide an explanation as to why they are so high?
The amount of BSA, as part of the formulation of a protein, can vary considerably depending on how much BSA was deemed optimum/necessary for protein stability in combination with /in-lieu of - other possible additives. The aforementioned formulations are somewhat analogous to the “carrier” versions of many formulations from “R and D systems” that have as high as 50 µg of BSA per µg of the recombinant protein product. If, needed or desired, abm scientists can substitute BSA for other stabilizing additives for most formulations.
Are your Escherichia coli sourced growth factors: 1) Human derived materials free? 2) Recombinant proteins free?
Yes, all of abm's growth factors made in Escherichia coli using recombinant technology contain no human derived-products or other recombinant proteins. In the rare cases of BSA presence, this will be mentioned in the product's formulation.