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Recombinant Human IL1B

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Product Source Size Cat. No. Price
Recombinant Human IL1B E. coli10 µg Z100395 $70.00
100 µg Z100397 $390.00
1.0 mg Z100399 $2,450.00
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Data Sheet
Print Version
Alternative Names Catabolin, Lymphocyte-activating factor, LAF, Endogenous Pyrogen, EP, IL1F2
Recombinant Human Interleukin-1 Beta (IL1B)
Description Monocytes are the main source of secreted IL1; expressing mostly IL1B, while human keratinocytes express large amounts of IL1A. Overall, IL1B is the predominant form in humans while, IL1A is the one in mice. Both isoforms of IL1 bind to the same receptor and therefore show similar, if not identical, biological activities. The IL1B precursor though must be processed before it can bind to the receptor. IL1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells. A monomer, IL1B stimulates thymocyte proliferation, B-cell maturation and proliferation and fibroblast growth factor activity.
Gene Symbol IL1B
Gene ID 3553
Accession No. P01584
Source E. coli
Appearance Lyophilized Powder
Molecular Weight 17.5 kDa
Endotoxin Level <1.0 EU/μg of recombinant protein as determined by the LAL method
Purity >95% as determined by SDS-PAGE
Gel Image Click To Enlarge
Bioactivity The ED50 as determined by the dose-dependent inhibition of prostate carcinoma cell line is less than 1ng/ml
Formulation Lyophilized from a 0.2 μm filtered solution in Tris, NaCl, EDTA and DTT (pH 8.0)
Reconstitution A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Storage The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage For research use only. Not for diagnostic or therapeutic use.
Product Documents
Product References
FAQs
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How are endotoxin levels measured?
1. For estimating the endotoxin levels; we use the LAL (Limulus Amebocyte Lysate) method: The lysate from horseshoe crab amebocytes clots in the presence of very low endotoxin. This reaction is the basis of the Limulus amebocyte lysate (LAL) assay which was approved by the FDA in 1970.

· Endotoxin is generally measured in Endotoxin Units per milliliter (EU/mL).

· For recombinant proteins: EU is reported per microgram of protein.

· One EU = 0.1-0.2 ng endotoxin/µg of protein.

· At abm, we do the LAL chromogenic assays that can detect down to 0.01 EU/ml.
With regard to the BSA levels in some Growth Factors and Cytokines, can you please provide an explanation as to why they are so high?
The amount of BSA, as part of the formulation of a protein, can vary considerably depending on how much BSA was deemed optimum/necessary for protein stability in combination with /in-lieu of - other possible additives. The aforementioned formulations are somewhat analogous to the “carrier” versions of many formulations from “R and D systems” that have as high as 50 µg of BSA per µg of the recombinant protein product. If, needed or desired, abm scientists can substitute BSA for other stabilizing additives for most formulations.
Are your Escherichia coli sourced growth factors: 1) Human derived materials free? 2) Recombinant proteins free?
Yes, all of abm's growth factors made in Escherichia coli using recombinant technology contain no human derived-products or other recombinant proteins. In the rare cases of BSA presence, this will be mentioned in the product's formulation.