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Recombinant Human IL27
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|Alternative Names||Interleukin-27 subunit alpha, IL-27 subunit alpha, IL-27-A, IL27-A, p28
Recombinant Human Interleukin-27 (IL27)
|Description||IL-27 is a cytokine with pro- and anti-inflammatory properties, that can regulate T helper cell development, suppress T cell proliferation, stimulate cytotoxic T cell activity, induce isotype switching in B cells, and that has diverse effects on innate immune cells. Among its target cells are CD4 T helper cells which can differentiate in type 1 effector cells (TH1), type 2 effector cells (TH2) and IL-17 producing helper T cells (TH17). It drives rapid clonal expansion of naive but not memory CD4 T cells. It also strongly synergizes with IL-12 to trigger interferon-gamma/IFN-gamma production of naive CD4 T cells, binds to the cytokine receptor WSX-1/TCCR. Another important role of IL-27 is its antitumor activity as well as its antiangiogenic activity with activation of production of antiangiogenic chemokines.|
|Molecular Weight||50.0 kDa|
|Endotoxin Level||<1.0 EU/μg of recombinant protein as determined by the LAL method.|
|Purity||>95% as determined by SDS-PAGE|
|Bioactivity||The activity was determined by its ability to bind recombinant human IL-27RA with a linear range of 60-1000 ng/mL in functional ELISA.|
|Formulation||Recombinant Human Interleukin-27 was lyophilized from a 0.2 μm filtered PBS solution pH 7.4.|
|Reconstitution||A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.|
|Storage||The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.|
|Usage||For research use only. Not for diagnostic or therapeutic use.|
· Endotoxin is generally measured in Endotoxin Units per milliliter (EU/mL).
· For recombinant proteins: EU is reported per microgram of protein.
· One EU = 0.1-0.2 ng endotoxin/µg of protein.
· At abm, we do the LAL chromogenic assays that can detect down to 0.01 EU/ml.