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Recombinant Human IL36G

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Product Source Size Cat. No. Price
Recombinant Human IL36G E. coli10 µg Z102215 $70.00
100 µg Z102217 $300.00
1.0 mg Z102219 $1,800.00
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Data Sheet
Print Version
Alternative Names IL1RP2, IL-1 epsilon, IL1F9, IL1H1
Description Human IL36G is a secreted member of the IL1 family, one of three IL36 genes in humans. The IL1 family members display a conserved 12 b-strand and a b-trefoil configuration, likely due to ascendance from a mutual ancenstral gene. IL36G is expressed primarily in tissues containing epithelial cells, and is both intracellular and secreted. Its primary receptor appears to be a combination of IL-1rp2 and IL-1Racp, the first being primarily expressed on dendritic cells and the second being more widely expressed. Binding of IL36G ligands to this receptor is one of the factors which induces dendritic cell maturation. Its expression is also an agonist for pro-inflammatory cytokines through the NF-kB and Map kinase pathways. Recombinant human IL36G migrates as an 18.9 kDa protein on SDS-PAGE and shares approximately 52% sequence identity with mouse IL36G.
Gene Symbol IL36G/IL1F9
Gene ID 56300
Accession No. Q9NZH8
Source E. coli
Appearance Lyophilized Powder
Molecular Weight 18.9 kDa
Endotoxin Level <1.0 EU/μg of recombinant protein as determined by the LAL method
Purity >95% as determined by SDS-PAGE
Gel Image Click To Enlarge
Bioactivity The ED(50) was determined by the dose-dependent proliferation of GieNo3B11 cells and was found to be <0.5ng/mL.
Bioactivity Data Click To Enlarge
Formulation Lyophilized from a 0.2 μm filtered solution in Tris and NaCl
Reconstitution A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Storage The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage For research use only. Not for diagnostic or therapeutic use.
Product Documents
Product References
FAQs
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How are endotoxin levels measured?
1. For estimating the endotoxin levels; we use the LAL (Limulus Amebocyte Lysate) method: The lysate from horseshoe crab amebocytes clots in the presence of very low endotoxin. This reaction is the basis of the Limulus amebocyte lysate (LAL) assay which was approved by the FDA in 1970.

· Endotoxin is generally measured in Endotoxin Units per milliliter (EU/mL).

· For recombinant proteins: EU is reported per microgram of protein.

· One EU = 0.1-0.2 ng endotoxin/µg of protein.

· At abm, we do the LAL chromogenic assays that can detect down to 0.01 EU/ml.
With regard to the BSA levels in some Growth Factors and Cytokines, can you please provide an explanation as to why they are so high?
The amount of BSA, as part of the formulation of a protein, can vary considerably depending on how much BSA was deemed optimum/necessary for protein stability in combination with /in-lieu of - other possible additives. The aforementioned formulations are somewhat analogous to the “carrier” versions of many formulations from “R and D systems” that have as high as 50 µg of BSA per µg of the recombinant protein product. If, needed or desired, abm scientists can substitute BSA for other stabilizing additives for most formulations.
Are your Escherichia coli sourced growth factors: 1) Human derived materials free? 2) Recombinant proteins free?
Yes, all of abm's growth factors made in Escherichia coli using recombinant technology contain no human derived-products or other recombinant proteins. In the rare cases of BSA presence, this will be mentioned in the product's formulation.