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Recombinant Human IL7
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Recombinant Human Interleukin-7 (IL7)
|Description||IL-7 is secreted constitutively into the conditioned medium of adherent bone marrow stromal cells and thymic cells. Mouse and human keratinocytes have been shown also to express and secrete IL7. Human IL-7 (152 amino acids; 17.4 kDa) and murine IL-7 (129 amino acids) show 60% sequence homology at the protein level. The human IL-7 receptor is an integral strongly glycosylated membrane proteins of 76 kDa expressed on activated T cells. This receptor has been designated as CD127. IL-7 receptors are expressed on pre-B cells and their progenitors. They are not expressed on mature B cells. IL-7 receptors are expressed also on bone marrow macrophages. Functional IL-7 receptors are found on the cell surface of multiphenotypic, biphenotypic, and immature lymphoid progenitors of B cells with the gene arrangement of the heavy immunoglobulin chain such as those observed in the germ line.|
|Molecular Weight||17.0 kDa|
|Endotoxin Level||<1.0 EU/μg of recombinant protein as determined by the LAL method.|
|Purity||>95% as determined by SDS-PAGE|
|Bioactivity||The ED(50) determined by dose-dependent stimulation of the proliferation of murine 2E8 cells is ≤ 0.5 ng/mL, corresponding to a specific activity of ≥ 2.0 x 10^6 units/mg.|
|Formulation||Recombinant Interleukin-7 was lyophilized from a 0.2 μm filtered PBS solution pH 4.5.|
|Reconstitution||A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.|
|Storage||The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.|
|Usage||For research use only. Not for diagnostic or therapeutic use.|
· Endotoxin is generally measured in Endotoxin Units per milliliter (EU/mL).
· For recombinant proteins: EU is reported per microgram of protein.
· One EU = 0.1-0.2 ng endotoxin/µg of protein.
· At abm, we do the LAL chromogenic assays that can detect down to 0.01 EU/ml.