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Recombinant Human IL8 (77aa)
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|Alternative Names||IL-8, C-X-C motif chemokine 8, Emoctakin, Granulocyte chemotactic protein 1, GCP-1, Monocyte-derived neutrophil chemotactic factor, MDNCF, Monocyte-derived neutrophil-activating peptide, MONAP, Neutrophil-activating protein 1, NAP-1, Protein 3-10C, T cell chemotactic factor
Recombinant Human Interleukin-8 (77AA) (IL8)
|Description||IL8 is produced by stimulated monocytes but not by tissue macrophages and T-lymphocytes. In many cell types the synthesis of IL8 is strongly stimulated by IL1, TNF-alpha and bacterial lipopolysaccharides. The expression of IL8 from resting and stimulated human blood monocytes is up-regulated by IL7. The IL8 receptor (CD128) is a dimeric glycoprotein consisting of a 59 kDa and a 67 kDa subunit. It is expressed in many different cell types including those not responding to IL8. IL8 differs from all other cytokines in its ability to specifically activate neutrophil granulocytes. IL8 also enhances the metabolism of reactive oxygen species and increases chemotaxis and the enhanced expression of adhesion molecules. Recombinant Human IL8 (77aa) represents the construct spanning residues 23 to 99, inclusive.|
|Molecular Weight||9.0 kDa|
|Endotoxin Level||<1.0 EU/μg of recombinant protein as determined by the LAL method|
|Purity||>95% as determined by SDS-PAGE|
|Bioactivity||The ED50 as determined by dose dependent proliferation of endothelial cells was found to be <0.5ng/ml|
|Formulation||Lyophilized from a 0.2 μm filtered solution in Phosphate buffer and NaCl (pH 7.5)|
|Reconstitution||A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers|
|Storage||The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.|
|Usage||For research use only. Not for diagnostic or therapeutic use.|
· Endotoxin is generally measured in Endotoxin Units per milliliter (EU/mL).
· For recombinant proteins: EU is reported per microgram of protein.
· One EU = 0.1-0.2 ng endotoxin/µg of protein.
· At abm, we do the LAL chromogenic assays that can detect down to 0.01 EU/ml.