Search Growth Factors & Cytokines By Gene Symbol
Recombinant Human Leptin (LEP)
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|Alternative Names||OB, Obese protein, Obesity factor|
|Description||Leptin is a cytokine found in the adipose tissue of animals (Leptin mRNA is restricted to the adipose tissue). Leptin has been shown to suppress appetite, increase thermogenesis and reduce body weight, food consumption and plasma glucose levels.|
|Molecular Weight||16.4 kDa|
|Endotoxin Level||<1.0 EU/μg of recombinant protein as determined by the LAL method|
|Purity||>95% as determined by SDS-PAGE|
|Bioactivity||The ED(50) was determined by the dose-dependent proliferation of human umbilical vein endothelial cells and was found to be <0.5ng/mL.|
|Formulation||Lyophilized from a 0.2 μm filtered solution in Tris-HCl and NaCl.|
|Reconstitution||Reconstitute at 1 mg/mL in sterile 20 mM Tris-HCI (pH 8.0).|
|Storage||The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.|
|Usage||For research use only. Not for diagnostic or therapeutic use.|
· Endotoxin is generally measured in Endotoxin Units per milliliter (EU/mL).
· For recombinant proteins: EU is reported per microgram of protein.
· One EU = 0.1-0.2 ng endotoxin/µg of protein.
· At abm, we do the LAL chromogenic assays that can detect down to 0.01 EU/ml.