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Recombinant Human MCP-2 (CCL8)
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|Alternative Names||Monocyte Chemotactic Protein-2, MCP2, HC14, C-C motif chemokine 8
Recombinant Human Monocyte Chemoattractant Protein-2 (CCL8)
|Description||Monocyte chemotactic protein MCP-2 is a C-C chemokine. It shares over 60% amino acid identity with MCP-1 and MCP- 3 and has about 30% identity with other C-C chemokines MIP-1 alpha, RANTES, and MIP-1 beta. MCP-2 is chemotactic for and activates a wide variety of inflammatory cells, and its spectrum of action on leukocytes is similar to that of MCP-3, including monocytes, T lymphocytes, natural killer cells, basophils, mast cells, and eosinophils, but differs from MCP-1, which is not active on eosinophils. MCP-2 has been proposed to interact with multiple C-C chemokine receptors, including those used by MCP- 1 and MCP-3. However, since leukocytes express a multiplicity of chemokine receptors with promiscuous binding and functional properties, it is difficult to identify the receptors used by a given ligand on these cells.|
|Molecular Weight||9.0 kDa|
|Endotoxin Level||<1.0 EU/μg of recombinant protein as determined by the LAL method|
|Purity||>95% as determined by SDS-PAGE|
|Bioactivity||The ED(50) was determined by the dose-dependent proliferation of Jurkat cells and was found to be <0.05ng/mL.|
|Formulation||Lyophilized from a 0.2 μm filtered 20 mM solution in PBS|
|Reconstitution||A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.|
|Storage||The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.|
|Usage||For research use only. Not for diagnostic or therapeutic use.|
· Endotoxin is generally measured in Endotoxin Units per milliliter (EU/mL).
· For recombinant proteins: EU is reported per microgram of protein.
· One EU = 0.1-0.2 ng endotoxin/µg of protein.
· At abm, we do the LAL chromogenic assays that can detect down to 0.01 EU/ml.