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Recombinant Human NRG4
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|Alternative Names||Pro-neuregulin-4, membrane-bound isoform, Pro-NRG4, NRG4
Recombinant Human Neuregulin-4 (NRG4)
|Description||Neuregulins (NDF, heregulin, GGF ARIA, or SMDF) are EGF-like growth and differentiation factors that signal through tyrosine kinase receptors of the ErbB family. The ErbB2 and ErbB4 receptors cooperate in transmission of neuregulin-1 signals in the heart, whereas ErbB2 and ErbB3 cooperate in neural crest cells.|
|Molecular Weight||7.0 kDa|
|Endotoxin Level||<1.0 EU/μg of recombinant protein as determined by the LAL method|
|Purity||>95% as determined by SDS-PAGE|
|Bioactivity||The ED(50) was determined by the dose-dependent proliferation of Raji cells and was found to be <0.05ng/mL.|
|Formulation||Lyophilized from a 0.2 μm filtered 20 mM PB, pH 6.0.|
|Reconstitution||A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.|
|Storage||The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.|
|Usage||For research use only. Not for diagnostic or therapeutic use.|
· Endotoxin is generally measured in Endotoxin Units per milliliter (EU/mL).
· For recombinant proteins: EU is reported per microgram of protein.
· One EU = 0.1-0.2 ng endotoxin/µg of protein.
· At abm, we do the LAL chromogenic assays that can detect down to 0.01 EU/ml.
Specific activity (Units/mg) = 10^6/ ED50 (ng/mL)
This formula was used to convert <3.0 ng/mL to 3.0 x 10^6 Units/mg.