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Recombinant Human Resistin (RETN)

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Product Source Size Cat. No. Price
Recombinant Human Resistin (RETN) E. coli25 µg Z100805 $70.00
1.0 mg Z100809 $1,250.00
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Data Sheet
Print Version
Alternative Names FIZZ3, Adipose tissue-specific secretory factor, ADSF, HXCP1, RSTIN, C/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein, Cysteine-rich secreted protein A12-alpha-like 2, Cysteine-rich secreted protein FIZZ3
Description Resistin was identified by screening for genes that are induced during the differentiation of adipocytes but down-regulated in mature adipocytes. Resistin gene expression is induced during the differentiation of adipocytes. Resistin circulates in mouse serum, and its level is increased markedly in obesity. Adipocytes secrete resistin as a unique signaling molecule that may be the hormone potentially linking obesity to type II diabetes, which is characterized by target-tissue resistance to insulin. TNF-alpha is a negative regulator of Resistin gene expression and inhibits Resistin mRNA expression and protein secretion by 70-90% in 3T3-L1 adipocytes.
Gene Symbol RETN
Gene ID 56729
Accession No. Q9HD89
Source E. coli
Appearance Lyophilized Powder
Molecular Weight 10.0 kDa
Endotoxin Level <1.0 EU/μg of recombinant protein as determined by the LAL method.
Purity >95% as determined by SDS-PAGE
Gel Image Click To Enlarge
Bioactivity Determined by its ability to stimulate lipolysis in cultured human adipocytes.
Bioactivity Data Click To Enlarge
Formulation Recombinant human Resistin was lyophilized from a 0.2 μm filtered PBS solution.
Reconstitution A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Storage The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage For research use only. Not for diagnostic or therapeutic use.
Product Documents
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FAQs
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How are endotoxin levels measured?
1. For estimating the endotoxin levels; we use the LAL (Limulus Amebocyte Lysate) method: The lysate from horseshoe crab amebocytes clots in the presence of very low endotoxin. This reaction is the basis of the Limulus amebocyte lysate (LAL) assay which was approved by the FDA in 1970.

· Endotoxin is generally measured in Endotoxin Units per milliliter (EU/mL).

· For recombinant proteins: EU is reported per microgram of protein.

· One EU = 0.1-0.2 ng endotoxin/µg of protein.

· At abm, we do the LAL chromogenic assays that can detect down to 0.01 EU/ml.
With regard to the BSA levels in some Growth Factors and Cytokines, can you please provide an explanation as to why they are so high?
The amount of BSA, as part of the formulation of a protein, can vary considerably depending on how much BSA was deemed optimum/necessary for protein stability in combination with /in-lieu of - other possible additives. The aforementioned formulations are somewhat analogous to the “carrier” versions of many formulations from “R and D systems” that have as high as 50 µg of BSA per µg of the recombinant protein product. If, needed or desired, abm scientists can substitute BSA for other stabilizing additives for most formulations.
Are your Escherichia coli sourced growth factors: 1) Human derived materials free? 2) Recombinant proteins free?
Yes, all of abm's growth factors made in Escherichia coli using recombinant technology contain no human derived-products or other recombinant proteins. In the rare cases of BSA presence, this will be mentioned in the product's formulation.