Recombinant Human SUMO Protease 1C

CAT.NOUNITPRICE
G696100 Units
$99.00

Specifications


Description

Recombinant Human SUMO Protease 1C is purified from E. coli by affinity chromatography using polyhistidine tag. The protease cleaves SUMO from recombinant fusion proteins in a highly specific manner. Following digestion, the SUMO protease is easily removed from the cleavage reaction by affinity chromatography using its polyhistidine tag fused at the N-terminus of the protease.

Components:

  • SUMO protease (1 U/μl): One unit of SUMO protease cleaves ≥85% of 2ug of substrate protein at 30°C in 1hour.
  • 10X SUMO protease buffer: 500μl

Special Features:

  • Recombinant human SUMO protease 1C in buffered aqueous solution
  • Highly active cleavage (ranges from 4°C to 37°C)
  • Highly specific cleavage, no non-specific proteolysis

Example Reaction to Start:

VolumeProduct Component
10 μl 10X SUMO protease buffer
20 μl Substrate SUMO-protein fusion
20 μl SUMO protease (1 unit/μl)
50 μl Water
100 μl Total Volume
SKUG696
Applications

 Removal of SUMO fusion tag from recombinant proteins.

Storage Condition

We recommend that the vial be briefly centrifuged prior to opening to bring the contents to the bottom. The stock solutions should be distributed into working aliquots and store at -20°C, or at -80°C for long-term storage. Avoid multiple freeze/thaw cycles at -80°C. Expires one year from date of receipt when stored as instructed.

Unit quantity100 Units
Documents


Supporting Protocol

    MSDS

      QC

        Other

          FAQs


          What is an Enzyme Unit defined as?
          One unit is defined as the amount of TEV Protease that is required to cleave >90% of 3 µg of control substrate in a 30 µl reaction for 1 hour at 30°C in 1X TEV Protease Reaction Buffer supplemented with 1 mM DTT.
          What buffer is the enzyme supplied with?
          Enzyme supplied with 20X Reaction Buffer.
          References


          There are no references for this product yet!