Recombinant Human SUMO Protease 1C

Recombinant Human SUMO Protease 1C is purified from E. coli by affinity chromatography using polyhistidine tag. The protease cleaves SUMO from recombinant fusion proteins in a highly specific manner. Following digestion, the SUMO protease is easily removed from the cleavage reaction by affinity chromatography using its polyhistidine tag fused at the N-terminus of the protease.

Components:

• SUMO protease (1 U/μl): One unit of SUMO protease cleaves ≥85% of 2ug of substrate protein at 30°C in 1hour.
• 10X SUMO protease buffer: 500μl

Special Features:

• Recombinant human SUMO protease 1C in buffered aqueous solution
• Highly active cleavage (ranges from 4°C to 37°C)
• Highly specific cleavage, no non-specific proteolysis

Example Reaction to Start:

 Removal of SUMO fusion tag from recombinant proteins.

We recommend that the vial be briefly centrifuged prior to opening to bring the contents to the bottom. The stock solutions should be distributed into working aliquots and store at -20°C, or at -80°C for long-term storage. Avoid multiple freeze/thaw cycles at -80°C. Expires one year from date of receipt when stored as instructed.