Recombinant Human SUMO Protease 1C
Recombinant HumanSUMO Protease 1C is purified from E.coli by affinity chromatography using polyhistidine tag. The protease cleaves SUMO from recombinant fusion proteins in a highly specific manner. Following digestion, SUMO protease is easily removed from the cleavage reaction by affinity chromatography using its polyhistidine tag fused at the N-terminus of the protease.
Removal of SUMO fusion tag from recombinant proteins.
We recommend that the vial be briefly centrifuged prior to opening to bring the contents to the bottom. The stock solutions should be distributed into working aliquots and store at -20°C, or at -80°C for long-term storage. Avoid multiple freeze/thaw cycles at -80°C. Expires one year from date of receipt when stored as instructed.
|Unit quantity||100 Units|
|What is an Enzyme Unit defined as?|
One unit is defined as the amount of TEV Protease that is required to cleave >90% of 3 µg of control substrate in a 30 µl reaction for 1 hour at 30°C in 1X TEV Protease Reaction Buffer supplemented with 1 mM DTT.
|What buffer is the enzyme supplied with?|
Enzyme supplied with 20X Reaction Buffer.
There are no references for this product yet!