Recombinant Human SUMO Protease 1C
| CAT.NO | UNIT | PRICE |
|---|---|---|
| G696 | 100 Units | $99.00 |
| Description | Recombinant HumanSUMO Protease 1C is purified from E.coli by affinity chromatography using polyhistidine tag. The protease cleaves SUMO from recombinant fusion proteins in a highly specific manner. Following digestion, SUMO protease is easily removed from the cleavage reaction by affinity chromatography using its polyhistidine tag fused at the N-terminus of the protease. Components
Special Features
|
|---|---|
| SKU | G696 |
| Applications | Removal of SUMO fusion tag from recombinant proteins. |
| Storage Condition | We recommend that the vial be briefly centrifuged prior to opening to bring the contents to the bottom. The stock solutions should be distributed into working aliquots and store at -20°C, or at -80°C for long-term storage. Avoid multiple freeze/thaw cycles at -80°C. Expires one year from date of receipt when stored as instructed. |
| Unit quantity | 100 Units |
Supporting Protocol
MSDS
QC
Other
| What is an Enzyme Unit defined as? | |
One unit is defined as the amount of TEV Protease that is required to cleave >90% of 3 µg of control substrate in a 30 µl reaction for 1 hour at 30°C in 1X TEV Protease Reaction Buffer supplemented with 1 mM DTT. | |
| What buffer is the enzyme supplied with? | |
Enzyme supplied with 20X Reaction Buffer. | |
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